scholarly journals Modifying Plant Photosynthesis and Growth via Simultaneous Chloroplast Transformation of Rubisco Large and Small Subunits

2020 ◽  
Vol 32 (9) ◽  
pp. 2898-2916 ◽  
Author(s):  
Elena Martin-Avila ◽  
Yi-Leen Lim ◽  
Rosemary Birch ◽  
Lynnette M.A. Dirk ◽  
Sally Buck ◽  
...  
Keyword(s):  
Chloroplast Transformation ◽  
Plant Photosynthesis

scholarly journals Improving recombinant Rubisco biogenesis, plant photosynthesis and growth by coexpressing its ancillary RAF1 chaperone

2015 ◽  
Vol 112 (11) ◽  
pp. 3564-3569 ◽  
Author(s):  
Spencer M. Whitney ◽  
Rosemary Birch ◽  
Celine Kelso ◽  
Jennifer L. Beck ◽  
Maxim V. Kapralov
Keyword(s):  
Steady State ◽  
Plant Growth ◽  
Adaptive Evolution ◽  
Resource Use ◽  
Chloroplast Transformation ◽  
Accumulation Factor ◽  
Mrna Levels ◽  
Assimilation Rate ◽  
Transplastomic Tobacco ◽  
Plant Photosynthesis

Enabling improvements to crop yield and resource use by enhancing the catalysis of the photosynthetic CO2-fixing enzyme Rubisco has been a longstanding challenge. Efforts toward realization of this goal have been greatly assisted by advances in understanding the complexities of Rubisco’s biogenesis in plastids and the development of tailored chloroplast transformation tools. Here we generate transplastomic tobacco genotypes expressing Arabidopsis Rubisco large subunits (AtL), both on their own (producing tobAtL plants) and with a cognate Rubisco accumulation factor 1 (AtRAF1) chaperone (producing tobAtL-R1 plants) that has undergone parallel functional coevolution with AtL. We show AtRAF1 assembles as a dimer and is produced in tobAtL-R1 and Arabidopsis leaves at 10–15 nmol AtRAF1 monomers per square meter. Consistent with a postchaperonin large (L)-subunit assembly role, the AtRAF1 facilitated two to threefold improvements in the amount and biogenesis rate of hybrid L8AS8t Rubisco [comprising AtL and tobacco small (S) subunits] in tobAtL-R1 leaves compared with tobAtL, despite >threefold lower steady-state Rubisco mRNA levels in tobAtL-R1. Accompanying twofold increases in photosynthetic CO2-assimilation rate and plant growth were measured for tobAtL-R1 lines. These findings highlight the importance of ancillary protein complementarity during Rubisco biogenesis in plastids, the possible constraints this has imposed on Rubisco adaptive evolution, and the likely need for such interaction specificity to be considered when optimizing recombinant Rubisco bioengineering in plants.

Heterosis in Upland Cotton. II. Relationship of Leaf Area to Plant Photosynthesis

Crop Science ◽  
1988 ◽  
Vol 28 (3) ◽  
pp. 522-525 ◽  
Author(s):  
Randy Wells ◽  
William R. Meredith ◽  
J. Ray Williford
Keyword(s):  
Leaf Area ◽  
Upland Cotton ◽  
Plant Photosynthesis ◽  
Relationship Of

Recombinant Protein Production in Microalgae: Emerging Trends

2020 ◽  
Vol 27 (2) ◽  
pp. 105-110 ◽  
Author(s):  
Niaz Ahmad ◽  
Muhammad Aamer Mehmood ◽  
Sana Malik
Keyword(s):  
Chloroplast Genome ◽  
Recombinant Proteins ◽  
Large Scale ◽  
Higher Plants ◽  
Scale Up ◽  
Chloroplast Transformation ◽  
Point Of View ◽  
Nuclear Transformation ◽  
Scale Production ◽  
Algal Chloroplast

: In recent years, microalgae have emerged as an alternative platform for large-scale production of recombinant proteins for different commercial applications. As a production platform, it has several advantages, including rapid growth, easily scale up and ability to grow with or without the external carbon source. Genetic transformation of several species has been established. Of these, Chlamydomonas reinhardtii has become significantly attractive for its potential to express foreign proteins inexpensively. All its three genomes – nuclear, mitochondrial and chloroplastic – have been sequenced. As a result, a wealth of information about its genetic machinery, protein expression mechanism (transcription, translation and post-translational modifications) is available. Over the years, various molecular tools have been developed for the manipulation of all these genomes. Various studies show that the transformation of the chloroplast genome has several advantages over nuclear transformation from the biopharming point of view. According to a recent survey, over 100 recombinant proteins have been expressed in algal chloroplasts. However, the expression levels achieved in the algal chloroplast genome are generally lower compared to the chloroplasts of higher plants. Work is therefore needed to make the algal chloroplast transformation commercially competitive. In this review, we discuss some examples from the algal research, which could play their role in making algal chloroplast commercially successful.

Life support system with autonomous control employing plant photosynthesis

1976 ◽  
Vol 3 (9-10) ◽  
pp. 633-650 ◽  
Author(s):  
I.I. Gitelson ◽  
I.A. Terskov ◽  
B.G. Kovrov ◽  
F.Ya. Sidko ◽  
G.M. Lisovsky ◽  
...  
Keyword(s):  
Support System ◽  
Life Support ◽  
Autonomous Control ◽  
Life Support System ◽  
Plant Photosynthesis

scholarly journals Studies on Chlamydomonas chloroplast transformation: foreign DNA can be stably maintained in the chromosome.

1989 ◽  
Vol 1 (1) ◽  
pp. 123-132 ◽  
Author(s):  
A D Blowers ◽  
L Bogorad ◽  
K B Shark ◽  
J C Sanford
Keyword(s):  
Chloroplast Transformation ◽  
Foreign Dna

scholarly journals Plant Photosynthesis-Irradiance Curve Responses to Pollution Show Non-Competitive Inhibited Michaelis Kinetics

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0142712 ◽  
Author(s):  
Maozi Lin ◽  
Zhiwei Wang ◽  
Lingchao He ◽  
Kang Xu ◽  
Dongliang Cheng ◽  
...  
Keyword(s):  
Plant Photosynthesis

Plant photosynthesis and its influence on removal efficiencies in constructed wetlands

2010 ◽  
Vol 36 (8) ◽  
pp. 1037-1043 ◽  
Author(s):  
Juan Huang ◽  
Shi-he Wang ◽  
Lu Yan ◽  
Qiu-shuang Zhong
Keyword(s):  
Constructed Wetlands ◽  
Plant Photosynthesis ◽  
Removal Efficiencies

OPC technique for in vivo studies in plant photosynthesis research

1992 ◽  
Vol 3 (9) ◽  
pp. 931-934 ◽  
Author(s):  
A C Pereira ◽  
M Zerbetto ◽  
G C Silva ◽  
H Vargas ◽  
W J da Silva ◽  
...  
Keyword(s):  
In Vivo Studies ◽  
Photosynthesis Research ◽  
Plant Photosynthesis

scholarly journals Enhancing (crop) plant photosynthesis by introducing novel genetic diversity

2017 ◽  
Vol 372 (1730) ◽  
pp. 20160380 ◽  
Author(s):  
Marcel Dann ◽  
Dario Leister
Keyword(s):  
Genetic Diversity ◽  
Genetic Manipulation ◽  
Plant Evolution ◽  
Crop Plants ◽  
Crop Plant ◽  
Higher Plant ◽  
Novel Approach ◽  
Population Sizes ◽  
Plant Photosynthesis ◽  
Successful Establishment

Although some elements of the photosynthetic light reactions might appear to be ideal, the overall efficiency of light conversion to biomass has not been optimized during evolution. Because crop plants are depleted of genetic diversity for photosynthesis, efforts to enhance its efficiency with respect to light conversion to yield must generate new variation. In principle, three sources of natural variation are available: (i) rare diversity within extant higher plant species, (ii) photosynthetic variants from algae, and (iii) reconstruction of no longer extant types of plant photosynthesis. Here, we argue for a novel approach that outsources crop photosynthesis to a cyanobacterium that is amenable to adaptive evolution. This system offers numerous advantages, including a short generation time, virtually unlimited population sizes and high mutation rates, together with a versatile toolbox for genetic manipulation. On such a synthetic bacterial platform, 10 000 years of (crop) plant evolution can be recapitulated within weeks. Limitations of this system arise from its unicellular nature, which cannot reproduce all aspects of crop photosynthesis. But successful establishment of such a bacterial host for crop photosynthesis promises not only to enhance the performance of eukaryotic photosynthesis but will also reveal novel facets of the molecular basis of photosynthetic flexibility. This article is part of the themed issue ‘Enhancing photosynthesis in crop plants: targets for improvement’.

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