scholarly journals AtTRAPPC11/ROG2: A Role for TRAPPs in Maintenance of the Plant Trans-Golgi Network/Early Endosome Organization and Function

2019 ◽  
Vol 31 (8) ◽  
pp. 1879-1898 ◽  
Author(s):  
Michel Ruiz Rosquete ◽  
Natasha Worden ◽  
Guangxi Ren ◽  
Rosalie M. Sinclair ◽  
Sina Pfleger ◽  
...  
Keyword(s):  
Early Endosome ◽  
Trans Golgi Network ◽  
And Function ◽  
Golgi Network

scholarly journals Plant trans-golgi network/early endosome pH regulation requires cation chloride cotransporter (CCC1)

eLife ◽  
2022 ◽  
Vol 11 ◽  
Author(s):  
Daniel Walter McKay ◽  
Heather E McFarlane ◽  
Yue Qu ◽  
Apriadi Situmorang ◽  
Matthew Gilliham ◽  
...  
Keyword(s):  
Ph Regulation ◽  
Early Endosome ◽  
Central Component ◽  
Pharmacological Treatments ◽  
Trans Golgi Network ◽  
Growth Defects ◽  
Function Loss ◽  
And Function ◽  
Golgi Network ◽  
Endocytic Pathways

Plant cells maintain a low luminal pH in the Trans-Golgi-Network/Early Endosome (TGN/EE), the organelle in which the secretory and endocytic pathways intersect. Impaired TGN/EE pH regulation translates into severe plant growth defects. The identity of the proton pump and proton/ion antiporters that regulate TGN/EE pH have been determined, but an essential component required to complete the TGN/EE membrane transport circuit remains unidentified - a pathway for cation and anion efflux. Here, we have used complementation, genetically encoded fluorescent sensors, and pharmacological treatments to demonstrate that Arabidopsis Cation Chloride Cotransporter (CCC1) is this missing component necessary for regulating TGN/EE pH and function. Loss of CCC1 function leads to alterations in TGN/EE-mediated processes including endocytic trafficking, exocytosis and response to abiotic stress, consistent with the multitude of phenotypic defects observed in ccc1 knockout plants. This discovery places CCC1 as a central component of plant cellular function.

scholarly journals Sphingomyelin homeostasis is required to form functional enzymatic domains at the trans-Golgi network

2014 ◽  
Vol 206 (5) ◽  
pp. 609-618 ◽  
Author(s):  
Josse van Galen ◽  
Felix Campelo ◽  
Emma Martínez-Alonso ◽  
Margherita Scarpa ◽  
José Ángel Martínez-Menárguez ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Hela Cells ◽  
Transmembrane Proteins ◽  
Short Chain ◽  
Membrane Domains ◽  
Trans Golgi Network ◽  
And Function ◽  
Golgi Network ◽  
Specific Membrane

Do lipids such as sphingomyelin (SM) that are known to assemble into specific membrane domains play a role in the organization and function of transmembrane proteins? In this paper, we show that disruption of SM homeostasis at the trans-Golgi network (TGN) by treatment of HeLa cells with d-ceramide-C6, which was converted together with phosphatidylcholine to short-chain SM and diacylglycerol by SM synthase, led to the segregation of Golgi-resident proteins from each other. We found that TGN46, which cycles between the TGN and the plasma membrane, was not sialylated by a sialyltransferase at the TGN and that this enzyme and its substrate TGN46 could not physically interact with each other. Our results suggest that SM organizes transmembrane proteins into functional enzymatic domains at the TGN.

BFA-induced compartments from the Golgi apparatus and trans-Golgi network/early endosome are distinct in plant cells

2009 ◽  
Vol 60 (5) ◽  
pp. 865-881 ◽  
Author(s):  
Sheung Kwan Lam ◽  
Yi Cai ◽  
Yu Chung Tse ◽  
Juan Wang ◽  
Angus Ho Yin Law ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Plant Cells ◽  
Early Endosome ◽  
Trans Golgi Network ◽  
Golgi Network

scholarly journals Interaction of the phospholipid flippase Drs2p with the F-box protein Rcy1p plays an important role in early endosome to trans-Golgi network vesicle transport in yeast

2013 ◽  
Vol 155 (1) ◽  
pp. 51-62 ◽  
Author(s):  
Hisatoshi Hanamatsu ◽  
Konomi Fujimura-Kamada ◽  
Takaharu Yamamoto ◽  
Nobumichi Furuta ◽  
Kazuma Tanaka
Keyword(s):  
Early Endosome ◽  
Vesicle Transport ◽  
Trans Golgi Network ◽  
F Box Protein ◽  
Golgi Network ◽  
Phospholipid Flippase
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