scholarly journals Functional Characterization of a Glycosyltransferase from the Moss Physcomitrella patens Involved in the Biosynthesis of a Novel Cell Wall Arabinoglucan

2018 ◽  
Vol 30 (6) ◽  
pp. 1293-1308 ◽  
Author(s):  
Alison W. Roberts ◽  
Jelle Lahnstein ◽  
Yves S.Y. Hsieh ◽  
Xiaohui Xing ◽  
Kuok Yap ◽  
...  
Keyword(s):  
Cell Wall ◽  
Physcomitrella Patens ◽  
Functional Characterization

scholarly journals In silico and functional characterization of the promoter of a Eucalyptussecondary cell wall associated cellulose synthase gene (EgCesA1)

2011 ◽  
Vol 5 (S7) ◽  
Author(s):  
Nicky Creux ◽  
Minique De Castro ◽  
Martin Ranik ◽  
Antanas Spokevicius ◽  
Gerd Bossinger ◽  
...  
Keyword(s):  
Cell Wall ◽  
In Silico ◽  
Functional Characterization ◽  
Cellulose Synthase ◽  
Cellulose Synthase Gene

scholarly journals Functional characterization of genes mediating cell wall metabolism and responses to plant cell wall integrity impairment

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Timo Engelsdorf ◽  
Lars Kjaer ◽  
Nora Gigli-Bisceglia ◽  
Lauri Vaahtera ◽  
Stefan Bauer ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Functional Characterization ◽  
Cell Wall Integrity ◽  
Cell Wall Metabolism

scholarly journals Functional Characterization of a Drought-Responsive Invertase Inhibitor from Maize (Zea mays L.)

2019 ◽  
Vol 20 (17) ◽  
pp. 4081 ◽  
Author(s):  
Lin Chen ◽  
Xiaohong Liu ◽  
Xiaojia Huang ◽  
Wei Luo ◽  
Yuming Long ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fluorescent Protein ◽  
Functional Characterization ◽  
Protein Fusion ◽  
Drought Treatment ◽  
Vacuolar Invertase ◽  
Maize Leaves ◽  
Green Fluorescent ◽  
Proteinaceous Inhibitor

Invertases (INVs) play essential roles in plant growth in response to environmental cues. Previous work showed that plant invertases can be post-translationally regulated by small protein inhibitors (INVINHs). Here, this study characterizes a proteinaceous inhibitor of INVs in maize (Zm-INVINH4). A functional analysis of the recombinant Zm-INVINH4 protein revealed that it inhibited both cell wall and vacuolar invertase activities from maize leaves. A Zm-INVINH4::green fluorescent protein fusion experiment indicated that this protein localized in the apoplast. Transcript analysis showed that Zm-INVINH4 is specifically expressed in maize sink tissues, such as the base part of the leaves and young kernels. Moreover, drought stress perturbation significantly induced Zm-INVINH4 expression, which was accompanied with a decrease of cell wall invertase (CWI) activities and an increase of sucrose accumulation in both base parts of the leaves 2 to 7 days after pollinated kernels. In summary, the results support the hypothesis that INV-related sink growth in response to drought treatment is (partially) caused by a silencing of INV activity via drought-induced induction of Zm-INVINH4 protein.

scholarly journals Functional Characterization of Class I Trehalose Biosynthesis Genes in Physcomitrella patens

2020 ◽  
Vol 10 ◽  
Author(s):  
Tran Le Cong Huyen Bao Phan ◽  
Ines Delorge ◽  
Nelson Avonce ◽  
Patrick Van Dijck
Keyword(s):  
Physcomitrella Patens ◽  
Functional Characterization ◽  
Class I ◽  
Trehalose Biosynthesis

scholarly journals Correction to: Functional characterization of genes mediating cell wall metabolism and responses to plant cell wall integrity impairment

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Timo Engelsdorf ◽  
Lars Kjaer ◽  
Nora Gigli-Bisceglia ◽  
Lauri Vaahtera ◽  
Stefan Bauer ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Functional Characterization ◽  
Cell Wall Integrity ◽  
Cell Wall Metabolism

Functional characterization of a cellulose synthase, CtCESA1, from the marine red alga Calliarthron tuberculosum (Corallinales)

2021 ◽  
Author(s):  
Jan Xue ◽  
Pallinti Purushotham ◽  
Justin F Acheson ◽  
Ruoya Ho ◽  
Jochen Zimmer ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacterial Species ◽  
Functional Characterization ◽  
Cellulose Synthase ◽  
Red Alga ◽  
Land Plant ◽  
Primary Cell Wall ◽  
Vitro Assay ◽  
Red Algal

Abstract In land plants and algae, cellulose is important for strengthening cell walls and preventing breakage due to physical forces. Though our understanding of cellulose production by cellulose synthase enzymes (CESAs) has seen significant advances for several land plant and bacterial species, functional characterization of this fundamental protein is absent in red algae. Here we identify CESA gene candidates in the calcifying red alga Calliarthron tuberculosum (Ct) using sequence similarity-based approaches and elucidate their phylogenetic relationship with other CESAs from diverse taxa. One gene candidate, CtCESA1, was closely related to other putative red algal CESAs. To test if CtCESA1 encoded a true cellulose synthase, CtCESA1 protein was expressed and purified from insect and yeast expression systems. CtCESA1 showed glucan synthase activity in glucose tracer assays. CtCESA1 activity was relatively low when compared to plant and bacterial CESA activity. In an in vitro assay, a predicted N-terminal starch-binding domain from CtCESA1 bound red algal floridean starch extracts, representing a unique domain in red algal CESAs not present in CESAs from other lineages. When the CtCESA1 gene was introduced into Arabidopsis thaliana cesa mutants, the red algal CtCESA1 partially rescued the growth defects of the primary cell wall cesa6 mutant, but not cesa3 or secondary cell wall cesa7 mutants. A fluorescently tagged CtCESA1 localized to the plasma membrane in the Arabidopsis cesa6 mutant background. This study presents functional evidence validating the sequence annotation of red algal cellulose synthases. The relatively low activity of CtCESA1, partial complementation in Arabidopsis, and presence of unique protein domains suggest that there are likely functional differences between the algal and land plant CESAs.

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