scholarly journals The Rice Receptor-Like Kinases DWARF AND RUNTISH SPIKELET1 and 2 Repress Cell Death and Affect Sugar Utilization during Reproductive Development

2017 ◽  
Vol 29 (1) ◽  
pp. 70-89 ◽  
Author(s):  
Cui-Xia Pu ◽  
Yong-Feng Han ◽  
Shu Zhu ◽  
Feng-Yan Song ◽  
Ying Zhao ◽  
...  
Keyword(s):  
Cell Death ◽  
Reproductive Development ◽  
Sugar Utilization

scholarly journals Transcriptional Regulation and Signaling of Developmental Programmed Cell Death in Plants

2021 ◽  
Vol 12 ◽  
Author(s):  
Cheng Jiang ◽  
Jiawei Wang ◽  
Hua-Ni Leng ◽  
Xiaqin Wang ◽  
Yijing Liu ◽  
...  
Keyword(s):  
Cell Death ◽  
Transcriptional Regulation ◽  
Programmed Cell Death ◽  
Industrial Production ◽  
Recent Progress ◽  
Reproductive Development ◽  
Genetic Networks ◽  
Molecular Regulation ◽  
Molecular Networks ◽  
Plant Growth And Development

Developmental programmed cell death (dPCD) has multiple functions in plant growth and development, and is of great value for industrial production. Among them, wood formed by xylem dPCD is one of the most widely used natural materials. Therefore, it is crucial to explore the molecular mechanism of plant dPCD. The dPCD process is tightly regulated by genetic networks and is involved in the transduction of signaling molecules. Several key regulators have been identified in diverse organisms and individual PCD events. However, complex molecular networks controlling plant dPCD remain highly elusive, and the original triggers of this process are still unknown. This review summarizes the recent progress on the transcriptional regulation and signaling of dPCD during vegetative and reproductive development. It is hoped that this review will provide an overall view of the molecular regulation of dPCD in different developmental processes in plants and identify specific mechanisms for regulating these dPCD events. In addition, the application of plants in industrial production can be improved by manipulating dPCD in specific processes, such as xylogenesis.

scholarly journals OsGPAT3 Plays a Critical Role in Anther Wall Programmed Cell Death and Pollen Development in Rice

2018 ◽  
Vol 19 (12) ◽  
pp. 4017 ◽  
Author(s):  
Lianping Sun ◽  
Xiaojiao Xiang ◽  
Zhengfu Yang ◽  
Ping Yu ◽  
Xiaoxia Wen ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Male Sterility ◽  
Critical Role ◽  
Pollen Grains ◽  
Reproductive Development ◽  
Anther Wall ◽  
Nutrient Metabolism ◽  
Pollen Maturation ◽  
Pollen Formation

In flowering plants, ideal male reproductive development requires the systematic coordination of various processes, in which timely differentiation and degradation of the anther wall, especially the tapetum, is essential for both pollen formation and anther dehiscence. Here, we show that OsGPAT3, a conserved glycerol-3-phosphate acyltransferase gene, plays a critical role in regulating anther wall degradation and pollen exine formation. The gpat3-2 mutant had defective synthesis of Ubisch bodies, delayed programmed cell death (PCD) of the inner three anther layers, and abnormal degradation of micropores/pollen grains, resulting in failure of pollen maturation and complete male sterility. Complementation and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) experiments demonstrated that OsGPAT3 is responsible for the male sterility phenotype. Furthermore, the expression level of tapetal PCD-related and nutrient metabolism-related genes changed significantly in the gpat3-2 anthers. Based on these genetic and cytological analyses, OsGPAT3 is proposed to coordinate the differentiation and degradation of the anther wall and pollen grains in addition to regulating lipid biosynthesis. This study provides insights for understanding the function of GPATs in regulating rice male reproductive development, and also lays a theoretical basis for hybrid rice breeding.

scholarly journals EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Ningning Niu ◽  
Wanqi Liang ◽  
Xijia Yang ◽  
Weilin Jin ◽  
Zoe A. Wilson ◽  
...  
Keyword(s):  
Cell Death ◽  
Tapetal Cell ◽  
Reproductive Development ◽  
Aspartic Proteases

scholarly journals The mitochondrial aldehyde dehydrogenase OsALDH2b negatively regulates tapetum degeneration in rice

2020 ◽  
Vol 71 (9) ◽  
pp. 2551-2560 ◽  
Author(s):  
Xianrong Xie ◽  
Zixu Zhang ◽  
Zhe Zhao ◽  
Yongyao Xie ◽  
Heying Li ◽  
...  
Keyword(s):  
Cell Death ◽  
Oryza Sativa ◽  
Programmed Cell Death ◽  
Aldehyde Dehydrogenase ◽  
Molecular Basis ◽  
Reproductive Development ◽  
Flowering Plants ◽  
Microspore Development ◽  
Tapetal Cells ◽  
Microspore Stage

Abstract Timely degradation of anther tapetal cells is a prerequisite for normal pollen development in flowering plants. Although several genes involved in tapetum development have been identified, the molecular basis of tapetum degeneration regulation remains poorly understood. In this study, we identified and characterized the nucleus-encoded, conserved mitochondrial aldehyde dehydrogenase OsALDH2b as a key regulator of tapetum degeneration in rice (Oryza sativa). OsALDH2b was highly expressed in anthers from meiosis to the early microspore stage. Mutation of OsALDH2b resulted in excess malonaldehyde accumulation and earlier programmed cell death in the tapetum, leading to premature tapetum degeneration and abnormal microspore development. These results demonstrate that OsALDH2b negatively regulates tapetal programmed cell death and is required for male reproductive development, providing insights into the regulation of tapetum development in plants.

DNA nick end labeling: a reliable marker for apoptosis?

1995 ◽  
Vol 53 ◽  
pp. 962-963
Author(s):  
Anne F. Bushnell ◽  
Sarah Webster ◽  
Lynn S. Perlmutter
Keyword(s):  
Cell Death ◽  
Cultured Cells ◽  
Apoptotic Cell ◽  
Morphological Characteristics ◽  
Detection Methods ◽  
Tissue Preparation ◽  
Preparation Methods ◽  
Dna Laddering ◽  
Disease States ◽  
Reliable Marker

Apoptosis, or programmed cell death, is an important mechanism in development and in diverse disease states. The morphological characteristics of apoptosis were first identified using the electron microscope. Since then, DNA laddering on agarose gels was found to correlate well with apoptotic cell death in cultured cells of dissimilar origins. Recently numerous DNA nick end labeling methods have been developed in an attempt to visualize, at the light microscopic level, the apoptotic cells responsible for DNA laddering.The present studies were designed to compare various tissue processing techniques and staining methods to assess the occurrence of apoptosis in post mortem tissue from Alzheimer's diseased (AD) and control human brains by DNA nick end labeling methods. Three tissue preparation methods and two commercial DNA nick end labeling kits were evaluated: the Apoptag kit from Oncor and the Biotin-21 dUTP 3' end labeling kit from Clontech. The detection methods of the two kits differed in that the Oncor kit used digoxigenin dUTP and anti-digoxigenin-peroxidase and the Clontech used biotinylated dUTP and avidinperoxidase. Both used 3-3' diaminobenzidine (DAB) for final color development.

Sign in / Sign up

Export Citation Format

Share Document