scholarly journals Environmental Nitrate Stimulates Abscisic Acid Accumulation in Arabidopsis Root Tips by Releasing It from Inactive Stores

2016 ◽  
Vol 28 (3) ◽  
pp. 729-745 ◽  
Author(s):  
Christine A. Ondzighi-Assoume ◽  
Sanhita Chakraborty ◽  
Jeanne M. Harris
Keyword(s):  
Abscisic Acid ◽  
Root Tips ◽  
Arabidopsis Root ◽  
Acid Accumulation

Dissecting the mechanism of abscisic acid-induced dynamic microtubule reorientation using live cell imaging

2013 ◽  
Vol 40 (3) ◽  
pp. 224 ◽  
Author(s):  
David Seung ◽  
Michael W. Webster ◽  
Richard Wang ◽  
Zornitza Andreeva ◽  
Jan Marc
Keyword(s):  
Abscisic Acid ◽  
Time Course ◽  
Underlying Mechanism ◽  
Allium Porrum ◽  
Root Tips ◽  
Arabidopsis Root ◽  
Microtubule Array ◽  
Microtubule Stability ◽  
Gfp Reporter ◽  
Microtubule Reorientation

Abscisic acid (ABA) is involved in plant development and responses to environmental stress including the formation of longitudinal microtubule arrays in elongating cells, although the underlying mechanism for this is unknown. We explored ABA-induced microtubule reorientation in leek (Allium porrum L.) leaf epidermal cells transiently expressing a GFP–MBD microtubule reporter. After 14–18 h incubation with ABA, the frequency of cells with longitudinal arrays of cortical microtubules along the outer epidermal wall increased with dose-dependency until saturation at 20 μM. Time-course imaging of individual cells revealed a gradual increase in the occurrence of discordant, dynamic microtubules deviating from the normal transverse microtubule array within 2–4 h of exposure to ABA, followed by reorientation into a completely longitudinal array within 5–8 h. Approximately one-half of the ABA-induced reorientation occurred independently of cytoplasmic streaming following the application of cytochalasin D. Reorientation occurred also in the elongation zone of Arabidopsis root tips. Transient expression of AtEB1b–GFP reporter and analysis of ‘comet’ velocities in Allium revealed that the microtubule growth rate increased by 55% within 3 h of exposure to ABA. ABA also increased the sensitivity of microtubules to depolymerisation by oryzalin and exacerbated oryzalin-induced radial swelling of Arabidopsis root tips. The swelling was further aggravated in AtPLDδ-null mutant, suggesting PLDδ plays a role in microtubule stability. We propose that ABA-induced reorientation of transverse microtubule array initially involves destabilisation of the array combined with the formation of dynamic, discordant microtubules.

Interaction between reactive oxygen species and nitric oxide in drought-induced abscisic acid synthesis in root tips of wheat seedlings

2001 ◽  
Vol 28 (10) ◽  
pp. 1055 ◽  
Author(s):  
Zhiguang Zhao ◽  
Guocang Chen ◽  
Chenglie Zhang
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Abscisic Acid ◽  
Reactive Oxygen ◽  
Acid Synthesis ◽  
Oxygen Species ◽  
Root Tips ◽  
Wheat Seedlings ◽  
Acid Accumulation ◽  
Oxide Synthase

Abscisic acid accumulation and oxidative stress are two common responses of plants to environmental stresses. However, little is known about their relationships. The purpose of this article is to investigate the effects of reactive oxygen species and nitric oxide on the plant hormone abscisic acid synthesis in root tips of wheat (Triticum aestivum L.) seedlings under drought stress. Detached root tips were subjected to drought stress by naturally evaporating until 20% of their fresh weights were lost. The activities of superoxide synthases and nitric oxide synthase (EC 1.14.13.39) increased after 20 min of treatment and abscisic acid began to accumulate 60 min later. The induction of abscisic acid by drought was strongly blocked by pretreating the root tips with reactive oxygen species eliminators tiron or ascorbate acid, and with nitric oxide synthase inhibitor Nω-nitro-L-arginine or nitric oxide eliminator 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl 3-oxide. Consistent with these results, reactive oxygen species generators diethyldithiocarbamic acid, xanthine–xanthine oxidase and triazole or nitric oxide donor sodium nitroprusside can also induce abscisic acid accumulation in root tips of wheat seedlings. While potentiated by reactive oxygen species, the effect of sodium nitroprusside on abscisic acid accumulation was blocked by 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl 3-oxide. Based on these results, we suggest that reactive oxygen species and nitric oxide play important roles in drought-induced abscisic acid synthesis in plant, they may be the signals through which the plant can ‘sense’ the drought condition.

Effects of abscisic acid and temperature on erucic acid accumulation in oilseed rape (Brassica napus L.)

1997 ◽  
Vol 150 (4) ◽  
pp. 414-419 ◽  
Author(s):  
Jeroen A. Wilmer ◽  
Johannes P.F.G. Helsper ◽  
Linus H.W. van der Plas
Keyword(s):  
Abscisic Acid ◽  
Brassica Napus ◽  
Erucic Acid ◽  
Oilseed Rape ◽  
Brassica Napus L ◽  
Acid Accumulation

QTL analysis to study the association between leaf size and abscisic acid accumulation in droughted rice leaves and comparisons across cereals

1997 ◽  
pp. 155-165 ◽  
Author(s):  
Stephen A. Quarrie ◽  
David A. Laurie ◽  
Jiahui Zhu ◽  
Claude Lebreton ◽  
Andrei Semikhodskii ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Qtl Analysis ◽  
Leaf Size ◽  
Acid Accumulation ◽  
Rice Leaves

Genetic and environmental effects on abscisic acid accumulation in leaves of field-grown maize

Euphytica ◽  
1994 ◽  
Vol 78 (1-2) ◽  
pp. 81-89
Author(s):  
S. Conti ◽  
P. Landi ◽  
M. C. Sanguineti ◽  
S. Stefanelli ◽  
R. Tuberosa
Keyword(s):  
Abscisic Acid ◽  
Environmental Effects ◽  
Acid Accumulation

scholarly journals Concentration-dependent effects of narciclasine on cell cycle progression in Arabidopsis root tips

2011 ◽  
Vol 11 (1) ◽  
pp. 184 ◽  
Author(s):  
Xiaofan Na ◽  
Yanfeng Hu ◽  
Kun Yue ◽  
Hongxia Lu ◽  
Pengfei Jia ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Root Tips ◽  
Cycle Progression ◽  
Arabidopsis Root
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