scholarly journals Cryptochrome Nucleocytoplasmic Distribution and Gene Expression Are Regulated by Light Quality in the Fern Adiantum capillus-veneris

2000 ◽  
Vol 12 (1) ◽  
pp. 81-95 ◽  
Author(s):  
Takato Imaizumi ◽  
Takeshi Kanegae ◽  
Masamitsu Wada
Keyword(s):  
Gene Expression ◽  
Light Quality ◽  
Adiantum Capillus Veneris ◽  
Nucleocytoplasmic Distribution

Effect of light quality on total gypenosides accumulation and related key enzyme gene expression in Gynostemma pentaphyllum

2018 ◽  
Vol 10 (1) ◽  
pp. 34-39
Author(s):  
Ting Wang ◽  
Xiang-rong Tian ◽  
Xiao-yu Wu ◽  
Zhun Luo ◽  
Gui Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Light Quality ◽  
Gynostemma Pentaphyllum ◽  
Enzyme Gene ◽  
Key Enzyme ◽  
Effect Of Light

Light quality modifies camptothecin production and gene expression of biosynthesis in Camptotheca acuminata Decne seedlings

2015 ◽  
Vol 66 ◽  
pp. 137-143 ◽  
Author(s):  
Yang Liu ◽  
Lili Song ◽  
Weiwu Yu ◽  
Yuanyuan Hu ◽  
Xiaohua Ma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Light Quality ◽  
Camptotheca Acuminata

Light quality affects flavonoid production and related gene expression in Cyclocarya paliurus

2018 ◽  
Vol 179 ◽  
pp. 66-73 ◽  
Author(s):  
Yang Liu ◽  
Shengzuo Fang ◽  
Wanxia Yang ◽  
Xulan Shang ◽  
Xiangxiang Fu
Keyword(s):  
Gene Expression ◽  
Light Quality ◽  
Related Gene ◽  
Cyclocarya Paliurus

scholarly journals Growth and Physiological Responses of Norway Spruce (Picea abies (L.) H. Karst) Supplemented with Monochromatic Red, Blue and Far-Red Light

Forests ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 164
Author(s):  
Fangqun OuYang ◽  
Yang Ou ◽  
Tianqin Zhu ◽  
Jianwei Ma ◽  
Sanping An ◽  
...  
Keyword(s):  
Gene Expression ◽  
Electron Transport ◽  
Picea Abies ◽  
Norway Spruce ◽  
Light Quality ◽  
Physiological Responses ◽  
Red Light ◽  
Variable Fluorescence ◽  
Related Gene ◽  
Bisphosphate Carboxylase

Monochromatic red light (R) supplementation is more efficient than blue light (B) in promoting Norway spruce (Picea abies (L.) H. Karst) growth. Transcriptome analysis has revealed that R and B may regulate stem growth by regulating phytohormones and secondary metabolites; however, the effects of light qualities on physiological responses and related gene expression in Norway spruce require further study. In the present study, three-year-old Norway spruce seedlings received sunlight during the daytime were exposed to monochromatic B (460 mm), monochromatic R (660 nm), monochromatic far-red light (FR, 730 nm), and a combination of three monochromatic lights (control, R:FR:B = 7:1:1) using light-emitting diode (LED) lamps for 12 h after sunset for 90 day. Growth traits, physiological responses, and related gene expression were determined. The results showed that light quality significantly affected Norway spruce growth. The stem height, root collar diameter, and current-year shoot length of seedlings treated with R were 2%, 10% and 12% higher, respectively, than those of the control, whereas seedlings treated with B and FR showed significantly lower values of these parameters compared with that of the control. The net photosynthetic rate (Pn) of seedlings under R treatment was 10% higher than that of the control, whereas the Pn values of seedlings treated with FR and B were 22% and 33%, respectively, lower than that of the control. The ratio of phosphoenolpyruvate carboxylase to ribulose-1,5-bisphosphate carboxylase/oxygenase (PEPC/Rubisco) of seedlings after the R treatment (0.581) was the highest and 3.98 times higher than that of the seedlings treated with B. Light quality significantly affected the gibberellic acid (GAs) levels, which was 13% higher in seedlings treated with R (6.4 g/100 ng) than that of the control, whereas, the GAs level of seedlings treated with B and FR was 17% and 19% lower, respectively, than that of the control. In addition, seedlings treated with R achieved the lowest ratio of leaf chlorophyll content to fresh weight (8.7). Compared to the R and control treatments, seedlings received FR treatment had consistently lower values of the quantum yield of electron transport beyond QA− (primary quinone, ϕEo) and efficiency, with which a trapped exciton moves an electron into the electron transport chain beyond QA− (ψo), while higher values of the relatively variable fluorescence at the J step and normalized relatively variable fluorescence at the K step (Wk). The values of ϕEo, ψO, VJ and Wk in seedlings treated with B were similar to those in the control group. The expression of genes associated with light signal transduction, such as PHYTOCHROME C (PHYC), ELONGATED HYPOCOTYL5 (HY5), CONSTITUTIVE PHOTOMORPHOGENIC 1-2 (COP1-2), and PHYTOCHROMEINTERACTING FACTOR 3 (PIF3), was significantly higher in seedlings under B treatment than those under other light treatments. Nevertheless, significant differences were not observed in the expression of COP1-2, HY5, and PIF3 between the R treatment and the control. The expression value of COP1-2 was significantly lower in R than FR light treatments. In conclusion, compared with the control, R promotes, whereas B and FR inhibit Norway spruce growth, which was accompanied by physiological changes and genes expression regulation that may be relate to a changing phytochrome photostationary state (PSS) with the supplemental R in seedlings.

scholarly journals Evaluation and Selection of Suitable qRT-PCR Reference Genes for Light Responses in Tea Plant (Camellia Sinensis)

2020 ◽  
Author(s):  
Jiaxin Wang ◽  
Qianhui Tang ◽  
Kang Sun ◽  
Liang Zeng ◽  
Zhijun Wu
Keyword(s):  
Gene Expression ◽  
Light Intensity ◽  
Camellia Sinensis ◽  
Reference Genes ◽  
Light Quality ◽  
Family Members ◽  
Tea Plant ◽  
Regulation Mechanism ◽  
Qrt Pcr ◽  
Selection Of

Abstract Background: Tea plant (Camellia sinensis) is an important woody economic crop used for processing leaf-type beverages. Tea has been proved to be beneficial to human health because it is rich in tea polyphenols and other active ingredients. Numerous studies have shown that light is a necessary environmental condition to control the growth and metabolism of C. sinensis. Gene expression experiments are always performed to explore the transcriptional regulation mechanism of plants widely based on the technique of quantitative real time polymerase chain reaction (qRT-PCR). The screening and application of reference genes are necessary for the normalization of gene expression under specific conditions. However, the reference genes for systematic analysis of light-induced transcription mechanisms are still not available in C. sinensis.Results: In this research, we identified actin family genes that are always used as reference genes with high frequency and without distinction for various expression experiments in C. sinensis. Six pairs of distinctive primers (corresponding to CsACT1, CsACT2, CsACT(3-4), CsACT(5-6), CsACT(7-8), and CsACT(9-10) genes) were designed to evaluate their expression stability in response to light quality (LQ), light intensity (LI), and photoperiod (PD). Simultaneously, six other family members (CsUBC1, CsCLATHRIN1, CsGAPDH, CsTBP, CsTIP41, and CseIF-4α) of C. sinensis commonly used as reference genes were also investigated. The stability rankings of gene expression were calculated by the statistical algorithms of geNorm, BestKeeper, NormFinder, and RefFinder softwares. Conclusions: CsACT(5-6), CsTIP41, and CsACT(3-4) were the most stable genes for light quality (LQ), light intensity (LI), and photoperiod (PD) treatments, respectively. This study provides a basis for the selection of reference genes for future research on the transcription mechanism of light response in C. sinensis. Moreover, the analysis of actin family members of C. sinensis will help to understand the individual transcription mechanism of housekeeping family.

scholarly journals Genetic and gene expression analysis of flowering time regulation by light quality in lentil

2021 ◽  
Author(s):  
Hai Ying Yuan ◽  
Carolyn T. Caron ◽  
Larissa Ramsay ◽  
Richard Fratini ◽  
Marcelino Pérez de la Vega ◽  
...  
Keyword(s):  
Gene Expression ◽  
Flowering Time ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Light Quality ◽  
Crop Improvement ◽  
Bhlh Transcription Factor ◽  
Phenotypic Variance ◽  
Potential Candidate ◽  
Subsequent Formation

AbstractFlowering time is important due to its roles in adaptation to different environments and subsequent formation of crop yield. Changes in light quality affect a range of developmental processes including flowering time, however little is known about light quality induced flowering time control in lentil. This study aims to investigate the genetic basis for differences in flowering response to light quality in lentil.We explored variation in flowering time caused by changes in red/far-red related light quality environments of a lentil interspecific recombinant inbred line population developed from a cross between Lens culinaris cv. Lupa and L. orientalis accession BGE 016880. A genetic linkage map was constructed and then used for identifying QTL associated with flowering time regulation under different light quality environments. Differential gene expression analysis through transcriptomic study and RT-qPCR were used to identify potential candidate genes.QTL mapping located 13 QTLs controlling flower time under different light quality environments, with phenotypic variance explained ranging from 1.7 to 62.9%. Transcriptomic profiling and gene expression analysis for both parents of this interspecific RIL population identified flowering-related genes showing environment-specific differential expression (flowering DEGs). One of these, a member of the florigen gene family FTa1 (LcFTa1) was located close to 3 major QTLs. Furthermore, gene expression results suggests two other florigen genes (LcFTb1 and LcFTb2), MADS-box transcription factors like LcAGL6/13d, LcSVPb, LcSOC1b and LcFULb, as well as bHLH transcription factor LcPIF6 and Gibberellin 20 oxidase LcGA20oxC,G, may be involved in the light quality response as well.Our results show that a major component of flowering time sensitivity to light quality is tightly linked to LcFTa1 and associated with changes in its expression. This work provides a foundation for crop improvement of lentil with better adaptation to variable light environments.

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