scholarly journals Unexpected Structure of Plant Promoters

2014 ◽  
Vol 26 (7) ◽  
pp. 2726-2726 ◽  
Author(s):  
Nancy A. Eckardt
Keyword(s):  
2010 ◽  
Vol 150 ◽  
pp. 531-531
Author(s):  
M.M. Rosa ◽  
S.M. Tauk-Tornisielo ◽  
M.L.R. Lopes-Assad ◽  
S.R. Ceccato-Antonini
Keyword(s):  

BMC Genomics ◽  
2018 ◽  
Vol 19 (S2) ◽  
Author(s):  
Chi-Nga Chow ◽  
Yi-Fan Chiang-Hsieh ◽  
Chia-Hung Chien ◽  
Han-Qin Zheng ◽  
Tzong-Yi Lee ◽  
...  

2013 ◽  
Vol 11 (01) ◽  
pp. 1340011 ◽  
Author(s):  
VICTORIA V. MIRONOVA ◽  
NADYA A. OMELYANCHUK ◽  
MARIA S. SAVINA ◽  
PETR M. PONOMARENKO ◽  
MIKHAIL P. PONOMARENKO ◽  
...  

Plant hormone auxin is a key regulator of growth and development. Auxin affects gene expression through ARF transcription factors, which bind specifically auxin responsive elements (AuxREs). Auxin responsive genes usually have more than one AuxRE, for example, a widely used auxin sensor DR5 contains seven AuxREs. Auxin responsive regions of several plant genes have been studied using sets of transgenic constructions in which the activity of one or several AuxREs were abolished. Here we present the method for analysis of the datasets on promoter activity assays having promoter sequences, namely, number and sequences of AuxREs, altogether with their measured auxin induction level. The method for a reverse problem solution considers two extreme models of AuxRE cooperation. Additive model describes auxin induction level of a gene as a sum of the individual AuxREs impacts. Multiplicative model considers pure cooperation between the AuxREs, where the combined effect is the multiplication of the individual AuxRE impacts. The reverse problem solution allows estimating the impact of an individual AuxRE into the induction level and the model for their cooperation. For promoters of three genes belonging to different plant species we showed that the multiplicative model fits better than additive. The reverse problem solution also suggests repressive state of auxin responsive promoters before auxin induction. The developed method provides possibility to investigate AuxRE structure-activity relationship and may be used as the basis for a novel approach for AuxRE recognition.


2020 ◽  
Vol 48 (21) ◽  
pp. 11845-11856 ◽  
Author(s):  
Yao-Min Cai ◽  
Kalyani Kallam ◽  
Henry Tidd ◽  
Giovanni Gendarini ◽  
Amanda Salzman ◽  
...  

Abstract Promoters serve a critical role in establishing baseline transcriptional capacity through the recruitment of proteins, including transcription factors. Previously, a paucity of data for cis-regulatory elements in plants meant that it was challenging to determine which sequence elements in plant promoter sequences contributed to transcriptional function. In this study, we have identified functional elements in the promoters of plant genes and plant pathogens that utilize plant transcriptional machinery for gene expression. We have established a quantitative experimental system to investigate transcriptional function, investigating how identity, density and position contribute to regulatory function. We then identified permissive architectures for minimal synthetic plant promoters enabling the computational design of a suite of synthetic promoters of different strengths. These have been used to regulate the relative expression of output genes in simple genetic devices.


2013 ◽  
Vol 3 (1) ◽  
Author(s):  
Yingchun Liu ◽  
Jiaming Yin ◽  
Meili Xiao ◽  
Annaliese S. Mason ◽  
Caihua Gao ◽  
...  
Keyword(s):  

2013 ◽  
Vol 8 (9) ◽  
pp. e25389 ◽  
Author(s):  
Ina Horst ◽  
Louisa Heimann ◽  
Christoph Peterhansel

2013 ◽  
Vol 56 (1) ◽  
pp. 38-49 ◽  
Author(s):  
Milena Silva Porto ◽  
Morganna Pollynne Nóbrega Pinheiro ◽  
Vandré Guevara Lyra Batista ◽  
Roseane Cavalcanti dos Santos ◽  
Péricles de Albuquerque Melo Filho ◽  
...  

2020 ◽  
Author(s):  
Yaomin Cai ◽  
Kalyani Kallam ◽  
Henry Tidd ◽  
Giovanni Gendarini ◽  
Amanda Salzman ◽  
...  

ABSTRACTPromoters serve a critical role in establishing baseline transcriptional capacity through the recruitment of proteins, including transcription factors (TFs). Previously, a paucity of data for cis-regulatory elements in plants meant that it was challenging to determine which sequence elements in plant promoter sequences contributed to transcriptional function. In this study, we have identified functional elements in the promoters of plant genes and plant pathogens that utilise plant transcriptional machinery for gene expression. We have established a quantitative experimental system to investigate transcriptional function, investigating how identity, density and position contribute to regulatory function. We then identified permissive architectures for minimal synthetic plant promoters enabling computational design of a suite of synthetic promoters of different strengths. These have been used to regulate the relative expression of output genes in simple genetic devices.


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