scholarly journals A Distal CCAAT/NUCLEAR FACTOR Y Complex Promotes Chromatin Looping at the FLOWERING LOCUS T Promoter and Regulates the Timing of Flowering in Arabidopsis

2014 ◽  
Vol 26 (3) ◽  
pp. 1009-1017 ◽  
Author(s):  
Shuanghe Cao ◽  
Roderick W. Kumimoto ◽  
Nerina Gnesutta ◽  
Alessandra M. Calogero ◽  
Roberto Mantovani ◽  
...  
Keyword(s):  
Nuclear Factor ◽  
Flowering Locus T ◽  
Nuclear Factor Y ◽  
Chromatin Looping ◽  
Flowering Locus

scholarly journals NUCLEAR FACTOR Y, subunit A (NF-YA) proteins positively regulate flowering and act through FLOWERING LOCUS T

2016 ◽  
Author(s):  
Chamindika L. Siriwardana ◽  
Nerina Gnesutta ◽  
Roderick W. Kumimoto ◽  
Daniel S. Jones ◽  
Zachary A. Myers ◽  
...  
Keyword(s):  
Reproductive Success ◽  
Plant Species ◽  
Strong Support ◽  
Day Length ◽  
Dependent Manner ◽  
Nuclear Factor ◽  
Flowering Locus T ◽  
Nuclear Factor Y ◽  
Timing Mechanisms ◽  
Flowering Locus

AbstractPhotoperiod dependent flowering is one of several mechanisms used by plants to initiate the developmental transition from vegetative growth to reproductive growth. The NUCLEAR FACTOR Y (NF-Y) transcription factors are heterotrimeric complexes composed of NF-YA and histone-fold domain (HFD) containing NF-YB/NF-YC, that initiate photoperiod-dependent flowering by cooperatively interacting with CONSTANS (CO) to drive the expression of FLOWERING LOCUS T (FT). This involves NF-Y and CO binding at distal CCAAT and proximal “CORE” elements, respectively, in the FT promoter. While this is well established for the HFD subunits, there remains some question over the potential role of NF-YA as either positive or negative regulators of this process. Here we provide strong support, in the form of genetic and biochemical analyses, that NF-YA, in complex with NF-YB/NF-YC proteins, can directly bind the distal CCAAT box in the FT promoter and are positive regulators of flowering in an FT-dependent manner.Author SummaryFor plants to have reproductive success, they must time their flowering with the most beneficial biotic and abiotic environmental conditions - after all, reproductive success would likely be low if flowers developed when pollinators were not present or freezing temperatures were on the horizon. Proper timing mechanisms for flowering vary significantly between different species, but can be connected to a variety of environmental cues, including water availability, temperature, and day length. Numerous labs have studied the molecular aspects of these timing mechanisms and discovered that many of these pathways converge on the gene FLOWERING LOCUS T (FT). This means that understanding precisely how this gene is regulated can teach us a lot about many plant species in both natural and agricultural settings. In the current study, we focus on day length as an essential cue for flowering in the plant species Arabidopsis thaliana. We further unravel the complexity of FT regulation by clarifying the roles of NUCLEAR FACTOR Y genes in day length perception.

scholarly journals NUCLEAR FACTOR Y, Subunit A (NF-YA) Proteins Positively Regulate Flowering and Act Through FLOWERING LOCUS T

PLoS Genetics ◽  
2016 ◽  
Vol 12 (12) ◽  
pp. e1006496 ◽  
Author(s):  
Chamindika L. Siriwardana ◽  
Nerina Gnesutta ◽  
Roderick W. Kumimoto ◽  
Daniel S. Jones ◽  
Zachary A. Myers ◽  
...  
Keyword(s):  
Nuclear Factor ◽  
Flowering Locus T ◽  
Subunit A ◽  
Nuclear Factor Y ◽  
Flowering Locus

scholarly journals A novel deletion in FLOWERING LOCUS T modulates flowering time in winter oilseed rape

2021 ◽  
Author(s):  
Paul Vollrath ◽  
Harmeet S. Chawla ◽  
Sarah V. Schiessl ◽  
Iulian Gabur ◽  
HueyTyng Lee ◽  
...  
Keyword(s):  
Association Mapping ◽  
Flowering Time ◽  
Oilseed Rape ◽  
Major Effect ◽  
Structural Variants ◽  
Winter Oilseed Rape ◽  
Flowering Locus T ◽  
Winter Type ◽  
Long Read ◽  
Flowering Locus

Abstract Key message A novel structural variant was discovered in the FLOWERING LOCUS T orthologue BnaFT.A02 by long-read sequencing. Nested association mapping in an elite winter oilseed rape population revealed that this 288 bp deletion associates with early flowering, putatively by modification of binding-sites for important flowering regulation genes. Abstract Perfect timing of flowering is crucial for optimal pollination and high seed yield. Extensive previous studies of flowering behavior in Brassica napus (canola, rapeseed) identified mutations in key flowering regulators which differentiate winter, semi-winter and spring ecotypes. However, because these are generally fixed in locally adapted genotypes, they have only limited relevance for fine adjustment of flowering time in elite cultivar gene pools. In crosses between ecotypes, the ecotype-specific major-effect mutations mask minor-effect loci of interest for breeding. Here, we investigated flowering time in a multiparental mapping population derived from seven elite winter oilseed rape cultivars which are fixed for major-effect mutations separating winter-type rapeseed from other ecotypes. Association mapping revealed eight genomic regions on chromosomes A02, C02 and C03 associating with fine modulation of flowering time. Long-read genomic resequencing of the seven parental lines identified seven structural variants coinciding with candidate genes for flowering time within chromosome regions associated with flowering time. Segregation patterns for these variants in the elite multiparental population and a diversity set of winter types using locus-specific assays revealed significant associations with flowering time for three deletions on chromosome A02. One of these was a previously undescribed 288 bp deletion within the second intron of FLOWERING LOCUS T on chromosome A02, emphasizing the advantage of long-read sequencing for detection of structural variants in this size range. Detailed analysis revealed the impact of this specific deletion on flowering-time modulation under extreme environments and varying day lengths in elite, winter-type oilseed rape.

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