scholarly journals Characterization of a NADH-Dependent Glutamate Dehydrogenase Mutant of Arabidopsis Demonstrates the Key Role of this Enzyme in Root Carbon and Nitrogen Metabolism

2012 ◽  
Vol 24 (10) ◽  
pp. 4044-4065 ◽  
Author(s):  
Jean-Xavier Fontaine ◽  
Thérèse Tercé-Laforgue ◽  
Patrick Armengaud ◽  
Gilles Clément ◽  
Jean-Pierre Renou ◽  
...  
Keyword(s):  
Glutamate Dehydrogenase ◽  
Nitrogen Metabolism ◽  
Carbon And Nitrogen ◽  
Root Carbon ◽  
Carbon And Nitrogen Metabolism

Cellular and subcellular localisation of glutamine synthetase and glutamate dehydrogenase in grapes gives new insights on the regulation of carbon and nitrogen metabolism

Planta ◽  
2002 ◽  
Vol 216 (2) ◽  
pp. 245-254 ◽  
Author(s):  
Virginie Paczek ◽  
Fréréric Dubois ◽  
Rajbir Sangwan ◽  
Jean-François Morot-Gaudry ◽  
Kalliopi Roubelakis-Angelakis ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Glutamate Dehydrogenase ◽  
Nitrogen Metabolism ◽  
Subcellular Localisation ◽  
Carbon And Nitrogen ◽  
Carbon And Nitrogen Metabolism

scholarly journals Evolution and regulation of nitrogen flux through compartmentalized metabolic networks in a marine diatom

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Sarah R. Smith ◽  
Chris L. Dupont ◽  
James K. McCarthy ◽  
Jared T. Broddrick ◽  
Miroslav Oborník ◽  
...  
Keyword(s):  
Nitrogen Metabolism ◽  
Phaeodactylum Tricornutum ◽  
Metabolic Networks ◽  
Nutrient Utilization ◽  
Marine Diatom ◽  
Carbon And Nitrogen ◽  
Flux Balance ◽  
Balance Analysis ◽  
Carbon And Nitrogen Metabolism

Abstract Diatoms outcompete other phytoplankton for nitrate, yet little is known about the mechanisms underpinning this ability. Genomes and genome-enabled studies have shown that diatoms possess unique features of nitrogen metabolism however, the implications for nutrient utilization and growth are poorly understood. Using a combination of transcriptomics, proteomics, metabolomics, fluxomics, and flux balance analysis to examine short-term shifts in nitrogen utilization in the model pennate diatom in Phaeodactylum tricornutum, we obtained a systems-level understanding of assimilation and intracellular distribution of nitrogen. Chloroplasts and mitochondria are energetically integrated at the critical intersection of carbon and nitrogen metabolism in diatoms. Pathways involved in this integration are organelle-localized GS-GOGAT cycles, aspartate and alanine systems for amino moiety exchange, and a split-organelle arginine biosynthesis pathway that clarifies the role of the diatom urea cycle. This unique configuration allows diatoms to efficiently adjust to changing nitrogen status, conferring an ecological advantage over other phytoplankton taxa.

scholarly journals The Effects of the Carbon Source on Glutamate Dehydrogenase Activities in Aspergillus nidulans

Microbiology ◽  
2000 ◽  
Vol 81 (1) ◽  
pp. 165-170 ◽  
Author(s):  
M. J. Hynes
Keyword(s):  
Protein Synthesis ◽  
Carbon Source ◽  
Glutamate Dehydrogenase ◽  
Aspergillus Nidulans ◽  
Nitrogen Metabolism ◽  
Wild Type Strain ◽  
Protein Synthesis Inhibitors ◽  
Carbon And Nitrogen ◽  
Glutamate Dehydrogenase Activity ◽  
Carbon And Nitrogen Metabolism

The NADP-specific glutamate dehydrogenase (NADP-GDH) activity of Aspergillus nidulans was rapidly lost from cultures starved for a carbon source. This loss of NADP-GDH was blocked by protein synthesis inhibitors. Glutamate repressed NADP-GDH but did not cause rapid loss of activity. Since NADP-GDH is involved in the participation of ammonium in the regulation of nitrogen metabolism, the loss of NADP-GDH activity accompanying carbon starvation may be important in the interaction between carbon and nitrogen metabolism. Increased NAD specific glutamate dehydrogenase activity (NAD-GDH) was observed when mycelium was transferred to medium lacking glucose. The increase in NAD-GDH activity was greatest when glutamate was present. Protein synthesis inhibitors did not prevent this increase in activity. Two mutants, amdT102 and amdT19, which are altered in regulation of nitrogen metabolism, are similar to the wild-type strain with regard to regulation of NADP-GDH and NAD-GDH.

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