scholarly journals Arabidopsis Chloroplast RNA Binding Proteins CP31A and CP29A Associate with Large Transcript Pools and Confer Cold Stress Tolerance by Influencing Multiple Chloroplast RNA Processing Steps

2012 ◽  
Vol 24 (10) ◽  
pp. 4266-4280 ◽  
Author(s):  
Christiane Kupsch ◽  
Hannes Ruwe ◽  
Sandra Gusewski ◽  
Michael Tillich ◽  
Ian Small ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Tolerance ◽  
Rna Processing ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Chloroplast Rna ◽  
Processing Steps

RNA-Binding Proteins Required for Chloroplast RNA Processing

2010 ◽  
pp. 177-203 ◽  
Author(s):  
Reimo Zoschke ◽  
Christiane Kupsch ◽  
Christian Schmitz-Linneweber
Keyword(s):  
Rna Processing ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Chloroplast Rna

scholarly journals Chloroplast RNA-binding proteins: Repair and regulation of chloroplast transcripts

RNA Biology ◽  
2010 ◽  
Vol 7 (2) ◽  
pp. 172-178 ◽  
Author(s):  
Michael Tillich ◽  
Susanne Beick ◽  
Christian Schmitz-Linneweber
Keyword(s):  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Chloroplast Rna ◽  
Chloroplast Transcripts

Use of plant chloroplast RNA-binding proteins as orthogonal activators of chloroplast transgenes in the green alga Chlamydomonas reinhardtii

2021 ◽  
Vol 60 ◽  
pp. 102535
Author(s):  
Noam Shahar ◽  
Tamar Elman ◽  
Rosalind Williams-Carrier ◽  
Oren Ben-Zvi ◽  
Iftach Yacoby ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Green Alga ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Plant Chloroplast ◽  
Chloroplast Rna

scholarly journals RNA-Binding Proteins in Amyotrophic Lateral Sclerosis and Neurodegeneration

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Scott E. Ugras ◽  
James Shorter
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Neurodegenerative Disease ◽  
Rna Processing ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Future Studies ◽  
Inclusion Formation ◽  
Als Patients ◽  
Lateral Sclerosis

Amyotrophic Lateral Sclerosis (ALS) is an adult onset neurodegenerative disease, which is universally fatal. While the causes of this devastating disease are poorly understood, recent advances have implicated RNA-binding proteins (RBPs) that contain predicted prion domains as a major culprit. Specifically, mutations in the RBPs TDP-43 and FUS can cause ALS. Cytoplasmic mislocalization and inclusion formation are common pathological features of TDP-43 and FUS proteinopathies. Though these RBPs share striking pathological and structural similarities, considerable evidence suggests that the ALS-linked mutations in TDP-43 and FUS can cause disease by disparate mechanisms. In a recent study, Couthouis et al. screened for protein candidates that were also involved in RNA processing, contained a predicted prion domain, shared other phenotypic similarities with TDP-43 and FUS, and identified TAF15 as a putative ALS gene. Subsequent sequencing of ALS patients successfully identified ALS-linked mutations in TAF15 that were largely absent in control populations. This study underscores the important role that perturbations in RNA metabolism might play in neurodegeneration, and it raises the possibility that future studies will identify other RBPs with critical roles in neurodegenerative disease.

Function of chloroplast RNA-binding proteins

2010 ◽  
Vol 68 (5) ◽  
pp. 735-748 ◽  
Author(s):  
Jessica Jacobs ◽  
Ulrich Kück
Keyword(s):  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Chloroplast Rna

scholarly journals Principles of RNA processing from analysis of enhanced CLIP maps for 150 RNA binding proteins

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Eric L. Van Nostrand ◽  
Gabriel A. Pratt ◽  
Brian A. Yee ◽  
Emily C. Wheeler ◽  
Steven M. Blue ◽  
...  
Keyword(s):  
Rna Processing ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins

scholarly journals The Chloroplast RNA Binding Protein CP31A Has a Preference for mRNAs Encoding the Subunits of the Chloroplast NAD(P)H Dehydrogenase Complex and Is Required for Their Accumulation

2020 ◽  
Vol 21 (16) ◽  
pp. 5633
Author(s):  
Benjamin Lenzen ◽  
Thilo Rühle ◽  
Marie-Kristin Lehniger ◽  
Ayako Okuzaki ◽  
Mathias Labs ◽  
...  
Keyword(s):  
Rna Processing ◽  
Gene Expression Analysis ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Binding Protein ◽  
Blot Hybridization ◽  
Rna Binding Protein ◽  
Target Range ◽  
Rna Targets ◽  
Chloroplast Rna

Chloroplast RNA processing requires a large number of nuclear-encoded RNA binding proteins (RBPs) that are imported post-translationally into the organelle. Most of these RBPs are highly specific for one or few target RNAs. By contrast, members of the chloroplast ribonucleoprotein family (cpRNPs) have a wider RNA target range. We here present a quantitative analysis of RNA targets of the cpRNP CP31A using digestion-optimized RNA co-immunoprecipitation with deep sequencing (DO-RIP-seq). This identifies the mRNAs coding for subunits of the chloroplast NAD(P)H dehydrogenase (NDH) complex as main targets for CP31A. We demonstrate using whole-genome gene expression analysis and targeted RNA gel blot hybridization that the ndh mRNAs are all down-regulated in cp31a mutants. This diminishes the activity of the NDH complex. Our findings demonstrate how a chloroplast RNA binding protein can combine functionally related RNAs into one post-transcriptional operon.

scholarly journals RNA-stabilization factors in chloroplasts of vascular plants

2018 ◽  
Vol 62 (1) ◽  
pp. 51-64 ◽  
Author(s):  
Nikolay Manavski ◽  
Lisa-Marie Schmid ◽  
Jörg Meurer
Keyword(s):  
Stress Responses ◽  
Vascular Plants ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Rna Degradation ◽  
Decay Rates ◽  
Transcriptional Level ◽  
Chloroplast Gene Expression ◽  
Chloroplast Rna

In contrast to the cyanobacterial ancestor, chloroplast gene expression is predominantly governed on the post-transcriptional level such as modifications of the RNA sequence, decay rates, exo- and endonucleolytic processing as well as translational events. The concerted function of numerous chloroplast RNA-binding proteins plays a fundamental and often essential role in all these processes but our understanding of their impact in regulation of RNA degradation is only at the beginning. Moreover, metabolic processes and post-translational modifications are thought to affect the function of RNA protectors. These protectors contain a variety of different RNA-recognition motifs, which often appear as multiple repeats. They are required for normal plant growth and development as well as diverse stress responses and acclimation processes. Interestingly, most of the protectors are plant specific which reflects a fast-evolving RNA metabolism in chloroplasts congruent with the diverging RNA targets. Here, we mainly focused on the characteristics of known chloroplast RNA-binding proteins that protect exonuclease-sensitive sites in chloroplasts of vascular plants.

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