scholarly journals Arabidopsis Defense against Botrytis cinerea: Chronology and Regulation Deciphered by High-Resolution Temporal Transcriptomic Analysis

2012 ◽  
Vol 24 (9) ◽  
pp. 3530-3557 ◽  
Author(s):  
Oliver Windram ◽  
Priyadharshini Madhou ◽  
Stuart McHattie ◽  
Claire Hill ◽  
Richard Hickman ◽  
...  
Keyword(s):  
High Resolution ◽  
Botrytis Cinerea ◽  
Transcriptomic Analysis ◽  
Arabidopsis Defense

scholarly journals Integrating High-Resolution and Solid-State Magic Angle Spinning NMR Spectroscopy and a Transcriptomic Analysis of Soybean Tissues in Response to Water Deficiency

2017 ◽  
Vol 28 (6) ◽  
pp. 529-540
Author(s):  
Isabel D. Coutinho ◽  
Tiago Bueno Moraes ◽  
Liliane Marcia Mertz-Henning ◽  
Alexandre Lima Nepomuceno ◽  
Willian Giordani ◽  
...  
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
High Resolution ◽  
Magic Angle Spinning ◽  
Transcriptomic Analysis ◽  
Magic Angle ◽  
Water Deficiency ◽  
Angle Spinning ◽  
Response To Water

scholarly journals Genome-wide Transcriptomic Analysis of the Response of Botrytis cinerea to Wuyiencin

2019 ◽  
Author(s):  
Liming Shi ◽  
Binghua Liu ◽  
Qiuhe Wei ◽  
Beibei Ge ◽  
Kecheng Zhang
Keyword(s):  
Biological Control ◽  
Cell Growth ◽  
Botrytis Cinerea ◽  
Target Genes ◽  
Biological Control Agent ◽  
Grey Mould ◽  
Control Agent ◽  
Transcriptomic Analysis ◽  
Genome Wide ◽  
A Genome

AbstractGrey mould is caused by the ascomycetes Botrytis cinerea in a range of crop hosts. As a biological control agent, the nucleoside antibiotic wuyiencin has been industrially produced and widely used as an effective fungicide. To elucidate the effects of wuyiencin on the transcriptional regulation in B. cinerea, we, for the first time, report a genome-wide transcriptomic analysis of B. cinerea treated with wuyiencin. We could identify 2067 differentially expressed genes (DEGs); of them, 886 and 1181 genes were significantly upregulated and downregulated, respectively. Functional categorization indicated that genes involved in amino acid metabolism and those encoding putative secreted proteins were remarkably influenced in response to wuyiencin treatment. Moreover, the expression of genes involved in protein synthesis and energy metabolism (oxidative phosphorylation) and of those encoding ATP-binding cassette transporters was markedly upregulated, whereas that of genes participating in DNA replication, cell cycle, and stress response was downregulated. Furthermore, wuyiencin resulted in mycelial malformation and negatively influenced cell growth rate and conidial yield in B. cinerea. Our results suggest that this nucleoside antibiotic regulates all aspects of cell growth and differentiation in B. cinerea. To summarize, we identified candidate pathways and target genes that may offer insights into the protective and antagonistic mechanisms underlying the action of biological control agents.

scholarly journals Genome-wide transcriptomic analysis of the response of Botrytis cinerea to wuyiencin

PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0224643
Author(s):  
Liming Shi ◽  
Binghua Liu ◽  
Qiuhe Wei ◽  
Beibei Ge ◽  
Kecheng Zhang
Keyword(s):  
Botrytis Cinerea ◽  
Transcriptomic Analysis ◽  
Genome Wide

Observations of the spatial structure of interstellar hydrogen

1967 ◽  
Vol 31 ◽  
pp. 45-46
Author(s):  
Carl Heiles
Keyword(s):  
High Resolution ◽  
Spatial Structure ◽  
Velocity Dispersion ◽  
Temperature Variations

High-resolution 21-cm line observations in a region aroundlII= 120°,b11= +15°, have revealed four types of structure in the interstellar hydrogen: a smooth background, large sheets of density 2 atoms cm-3, clouds occurring mostly in groups, and ‘Cloudlets’ of a few solar masses and a few parsecs in size; the velocity dispersion in the Cloudlets is only 1 km/sec. Strong temperature variations in the gas are in evidence.

Combining integrated systems-biology approaches with intervention-based experimental design provides a higher-resolution path forward for microbiome research

2019 ◽  
Vol 42 ◽  
Author(s):  
J. Alfredo Blakeley-Ruiz ◽  
Carlee S. McClintock ◽  
Ralph Lydic ◽  
Helen A. Baghdoyan ◽  
James J. Choo ◽  
...  
Keyword(s):  
Systems Biology ◽  
High Resolution ◽  
Experimental Design ◽  
Integrated Systems ◽  
Microbiome Research ◽  
Constructive Criticism

Abstract The Hooks et al. review of microbiota-gut-brain (MGB) literature provides a constructive criticism of the general approaches encompassing MGB research. This commentary extends their review by: (a) highlighting capabilities of advanced systems-biology “-omics” techniques for microbiome research and (b) recommending that combining these high-resolution techniques with intervention-based experimental design may be the path forward for future MGB research.

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