scholarly journals The IMMUTANS Variegation Locus of Arabidopsis Defines a Mitochondrial Alternative Oxidase Homolog That Functions during Early Chloroplast Biogenesis

1999 ◽  
Vol 11 (1) ◽  
pp. 43-55 ◽  
Author(s):  
Dongying Wu ◽  
David A. Wright ◽  
Carolyn Wetzel ◽  
Daniel F. Voytas ◽  
Steven Rodermel
Keyword(s):  
Alternative Oxidase ◽  
Chloroplast Biogenesis

scholarly journals The IMMUTANS Variegation Locus of Arabidopsis Defines a Mitochondrial Alternative Oxidase Homolog That Functions during Early Chloroplast Biogenesis

1999 ◽  
Vol 11 (1) ◽  
pp. 43 ◽  
Author(s):  
Dongying Wu ◽  
David A. Wright ◽  
Carolyn Wetzel ◽  
Daniel F. Voytas ◽  
Steven Rodermel
Keyword(s):  
Alternative Oxidase ◽  
Chloroplast Biogenesis

Alternative oxidase from Arum and soybean: Its stabilization during purification

1996 ◽  
Vol 96 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Qisen Zhang ◽  
Marcel H. N. Hoefnagel ◽  
Joseph T. Wiskich
Keyword(s):  
Alternative Oxidase

GATL12 is Essential for Chloroplast Biogenesis in Arabidopsis

2011 ◽  
Vol 46 (4) ◽  
pp. 379-385 ◽  
Author(s):  
Su Yanping ◽  
Lu Xiaoduo ◽  
Shen Songdong ◽  
Zhang Chunyi
Keyword(s):  
Chloroplast Biogenesis

Multiple effects of inhibition of mitochondrial alternative oxidase pathway on photosynthetic apparatus in Rumex K-1 leaves

2012 ◽  
Vol 56 (2) ◽  
pp. 365-368 ◽  
Author(s):  
L.T. Zhang ◽  
H.Y. Gao ◽  
Z.S. Zhang ◽  
Z.C. Xue ◽  
Q.W. Meng
Keyword(s):  
Alternative Oxidase ◽  
Photosynthetic Apparatus

scholarly journals Alternative oxidase encoded by sequence-optimized and chemically-modified RNA transfected into mammalian cells is catalytically active

Gene Therapy ◽  
2021 ◽  
Author(s):  
Luca Giordano ◽  
Manish K. Aneja ◽  
Natascha Sommer ◽  
Nasim Alebrahimdehkordi ◽  
Alireza Seraji ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Alternative Oxidase ◽  
Chemically Modified ◽  
Lung Carcinoma Cells ◽  
Primary Mouse ◽  
Catalytically Active ◽  
Human Lung Carcinoma ◽  
Pulmonary Arterial ◽  
Metabolic Stresses ◽  
Mitochondrial Respiratory

AbstractPlants and other organisms, but not insects or vertebrates, express the auxiliary respiratory enzyme alternative oxidase (AOX) that bypasses mitochondrial respiratory complexes III and/or IV when impaired. Persistent expression of AOX from Ciona intestinalis in mammalian models has previously been shown to be effective in alleviating some metabolic stresses produced by respiratory chain inhibition while exacerbating others. This implies that chronic AOX expression may modify or disrupt metabolic signaling processes necessary to orchestrate adaptive remodeling, suggesting that its potential therapeutic use may be confined to acute pathologies, where a single course of treatment would suffice. One possible route for administering AOX transiently is AOX-encoding nucleic acid constructs. Here we demonstrate that AOX-encoding chemically-modified RNA (cmRNA), sequence-optimized for expression in mammalian cells, was able to support AOX expression in immortalized mouse embryonic fibroblasts (iMEFs), human lung carcinoma cells (A549) and primary mouse pulmonary arterial smooth muscle cells (PASMCs). AOX protein was detectable as early as 3 h after transfection, had a half-life of ~4 days and was catalytically active, thus supporting respiration and protecting against respiratory inhibition. Our data demonstrate that AOX-encoding cmRNA optimized for use in mammalian cells represents a viable route to investigate and possibly treat mitochondrial respiratory disorders.

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