scholarly journals Internal Architecture of Mitochondrial Complex I from Arabidopsis thaliana

2010 ◽  
Vol 22 (3) ◽  
pp. 797-810 ◽  
Author(s):  
Jennifer Klodmann ◽  
Stephanie Sunderhaus ◽  
Manfred Nimtz ◽  
Lothar Jänsch ◽  
Hans-Peter Braun
Keyword(s):  
Arabidopsis Thaliana ◽  
Complex I ◽  
Mitochondrial Complex I ◽  
Mitochondrial Complex ◽  
Internal Architecture

scholarly journals A ferredoxin bridge connects the two arms of plant mitochondrial complex I

2020 ◽  
Author(s):  
Niklas Klusch ◽  
Jennifer Senkler ◽  
Özkan Yildiz ◽  
Werner Kühlbrandt ◽  
Hans-Peter Braun
Keyword(s):  
Arabidopsis Thaliana ◽  
Complex I ◽  
Mitochondrial Membrane ◽  
Mitochondrial Complex I ◽  
Mitochondrial Complex ◽  
Inner Mitochondrial Membrane ◽  
Main Site ◽  
The Matrix ◽  
Quinol Binding ◽  
Complex I Activity

SUMMARYMitochondrial complex I is the main site for electron transfer to the respiratory chain and generates much of the proton gradient across the inner mitochondrial membrane. It is composed of two arms, which form a conserved L-shape. We report the structures of the intact, 47-subunit mitochondrial complex I from Arabidopsis thaliana and from the green alga Polytomella sp. at 3.2 and 3.3 Å resolution. In both, a heterotrimeric γ-carbonic anhydrase domain is attached to the membrane arm on the matrix side. Two states are resolved in A. thaliana complex I, with different angles between the two arms and different conformations of the ND1 loop near the quinol binding site. The angle appears to depend on a bridge domain, which links the peripheral arm to the membrane arm and includes an unusual ferredoxin. We suggest that the bridge domain regulates complex I activity.One sentence summaryThe activity of complex I depends on the angel between its two arms, which, in plants, is adjusted by a protein bridge that includes an unusual ferredoxin.The authors responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) are: Hans-Peter Braun ([email protected]) and Werner Kühlbrandt ([email protected]).

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