scholarly journals Nuclear Activity of ROXY1, a Glutaredoxin Interacting with TGA Factors, Is Required for Petal Development in Arabidopsis thaliana

2009 ◽  
Vol 21 (2) ◽  
pp. 429-441 ◽  
Author(s):  
Shutian Li ◽  
Andrea Lauri ◽  
Mark Ziemann ◽  
Andrea Busch ◽  
Mrinal Bhave ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Nuclear Activity ◽  
Petal Development ◽  
Tga Factors

scholarly journals Control of flower development in Arabidopsis thaliana by APETALA1 and interacting genes

Development ◽  
1993 ◽  
Vol 119 (3) ◽  
pp. 721-743 ◽  
Author(s):  
J. L. Bowman ◽  
J. Alvarez ◽  
D. Weigel ◽  
E. M. Meyerowitz ◽  
D. R. Smyth
Keyword(s):  
Arabidopsis Thaliana ◽  
In Situ Hybridization ◽  
Flower Development ◽  
Floral Organ ◽  
Allelic Series ◽  
Flower Meristem ◽  
Petal Development ◽  
Two Phases ◽  
Floral Organ Specification

Mutations in the APETALA1 gene disturb two phases of flower development, flower meristem specification and floral organ specification. These effects become manifest as a partial conversion of flowers into inflorescence shoots and a disruption of sepal and petal development. We describe the changes in an allelic series of nine apetala1 mutants and show that the two functions of APETALA1 are separable. We have also studied the interaction between APETALA1 and other floral genes by examining the phenotypes of multiply mutant plants and by in situ hybridization using probes for several floral control genes. The results suggest that the products of APETALA1 and another gene, LEAFY, are required to ensure that primordia arising on the flanks of the inflorescence apex adopt a floral fate, as opposed to becoming an inflorescence shoot. APETALA1 and LEAFY have distinct as well as overlapping functions and they appear to reinforce each other's action. CAULIFLOWER is a newly discovered gene which positively regulates both APETALA1 and LEAFY expression. All functions of CAULIFLOWER are redundant with those of APETALA1. APETALA2 also has an early function in reinforcing the action of APETALA1 and LEAFY, especially if the activity of either is compromised by mutation. After the identity of a flower primordium is specified, APETALA1 interacts with APETALA2 in controlling the development of the outer two whorls of floral organs.

scholarly journals COP1 is required for UV-B–induced nuclear accumulation of the UVR8 photoreceptor

2016 ◽  
Vol 113 (30) ◽  
pp. E4415-E4422 ◽  
Author(s):  
Ruohe Yin ◽  
Mariya Y. Skvortsova ◽  
Sylvain Loubéry ◽  
Roman Ulm
Keyword(s):  
Arabidopsis Thaliana ◽  
Glucocorticoid Receptor ◽  
Fusion Protein ◽  
Nuclear Localization ◽  
Dual Role ◽  
Nuclear Accumulation ◽  
Resistance Locus ◽  
Uv Resistance ◽  
Nuclear Activity ◽  
Main Activity

The UV-B photoreceptor UV RESISTANCE LOCUS 8 (UVR8) promotes UV-B acclimation and tolerance in Arabidopsis thaliana. UVR8 localizes to both cytosol and nucleus, but its main activity is assumed to be nuclear. UV-B photoreception stimulates nuclear accumulation of UVR8 in a presently unknown manner. Here, we show that CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) is required for UV-B–induced nuclear accumulation of UVR8, but bypassing the COP1 requirement for UVR8 nuclear accumulation did not rescue the cop1 mutant UV-B phenotype. Using a glucocorticoid receptor (GR)-based fusion protein system to conditionally localize GR-UVR8 to the nucleus, we have demonstrated that both photoactivation and nuclear localization of UVR8 are required for UV-B–induced photomorphogenic responses. In contrast, there was no UV-B response when UV-B–activated UVR8 was artificially retained in the cytosol. In agreement with a predominantly nuclear activity, constitutively active UVR8W285A accumulated in the nucleus also in the absence of UV-B. Furthermore, GR-COP1 expression lines suggested that UV-B–activated UVR8 can be coimported into the nucleus by COP1. Our data strongly support localization of UVR8 signaling in the nucleus and a dual role for COP1 in the regulation of UV-B–induced UVR8 nuclear accumulation and in UVR8-mediated UV-B signaling.

Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta

1994 ◽  
Vol 5 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Seok So Chang ◽  
Soon Ki Park ◽  
Byung Chul Kim ◽  
Bong Joong Kang ◽  
Dal Ung Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Genetic Transformation ◽  
In Planta
Sign in / Sign up

Export Citation Format

Share Document