scholarly journals Crosstalk between Cold Response and Flowering in Arabidopsis Is Mediated through the Flowering-Time Gene SOC1 and Its Upstream Negative Regulator FLC

2009 ◽  
Vol 21 (10) ◽  
pp. 3185-3197 ◽  
Author(s):  
Eunjoo Seo ◽  
Horim Lee ◽  
Jin Jeon ◽  
Hanna Park ◽  
Jungmook Kim ◽  
...  
Keyword(s):  
Flowering Time ◽  
Negative Regulator ◽  
Cold Response ◽  
Flowering Time Gene

Functional importance of conserved domains in the flowering-time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants

2002 ◽  
Vol 28 (6) ◽  
pp. 619-631 ◽  
Author(s):  
Frances Robson ◽  
M. Manuela R Costa ◽  
Shelley R Hepworth ◽  
Igor Vizir ◽  
Manuel Pin˜eiro ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Flowering Time ◽  
Functional Importance ◽  
Conserved Domains ◽  
Flowering Time Gene

Cloning and characterization of a flowering time gene fromThellungiella halophila

2008 ◽  
Vol 40 (8) ◽  
pp. 747-753 ◽  
Author(s):  
Qiaoyun Fang ◽  
Jun Liu ◽  
Zhengkai Xu ◽  
Rentao Song
Keyword(s):  
Flowering Time ◽  
Flowering Time Gene

scholarly journals Dehydroabietinal promotes flowering time and plant defense in Arabidopsis via the autonomous pathway genes FLOWERING LOCUS D, FVE, and RELATIVE OF EARLY FLOWERING 6

2020 ◽  
Vol 71 (16) ◽  
pp. 4903-4913 ◽  
Author(s):  
Zulkarnain Chowdhury ◽  
Devasantosh Mohanty ◽  
Mrunmay K Giri ◽  
Barney J Venables ◽  
Ratnesh Chaturvedi ◽  
...  
Keyword(s):  
Flowering Time ◽  
Systemic Acquired Resistance ◽  
Defense Mechanism ◽  
Acquired Resistance ◽  
Early Flowering ◽  
Negative Regulator ◽  
Flowering Locus T ◽  
Autonomous Pathway ◽  
Abietane Diterpenoids ◽  
Flowering Locus

Abstract Abietane diterpenoids are tricyclic diterpenes whose biological functions in angiosperms are largely unknown. Here, we show that dehydroabietinal (DA) fosters transition from the vegetative phase to reproductive development in Arabidopsis thaliana by promoting flowering time. DA’s promotion of flowering time was mediated through up-regulation of the autonomous pathway genes FLOWERING LOCUS D (FLD), RELATIVE OF EARLY FLOWERING 6 (REF6), and FVE, which repress expression of FLOWERING LOCUS C (FLC), a negative regulator of the key floral integrator FLOWERING LOCUS T (FT). Our results further indicate that FLD, REF6, and FVE are also required for systemic acquired resistance (SAR), an inducible defense mechanism that is also activated by DA. However, unlike flowering time, FT was not required for DA-induced SAR. Conversely, salicylic acid, which is essential for the manifestation of SAR, was not required for the DA-promoted flowering time. Thus, although the autonomous pathway genes FLD, REF6, and FVE are involved in SAR and flowering time, these biological processes are not interdependent. We suggest that SAR and flowering time signaling pathways bifurcate at a step downstream of FLD, REF6, and FVE, with an FLC-dependent arm controlling flowering time, and an FLC-independent pathway controlling SAR.

scholarly journals Overexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of FT expression

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Reuben Tayengwa ◽  
Pushpa Sharma Koirala ◽  
Courtney F. Pierce ◽  
Breanna E. Werner ◽  
Michael M. Neff
Keyword(s):  
Transgenic Plants ◽  
Gene Family ◽  
Flowering Time ◽  
Camelina Sativa ◽  
Negative Regulator ◽  
Peptide Sequence ◽  
Type I ◽  
Mrna Levels ◽  
Core Motif ◽  
Late Flowering

Abstract Background The 29-member Arabidopsis AHL gene family is classified into three main classes based on nucleotide and protein sequence evolutionary differences. These differences include the presence or absence of introns, type and/or number of conserved AT-hook and PPC domains. AHL gene family members are divided into two phylogenetic clades, Clade-A and Clade-B. A majority of the 29 members remain functionally uncharacterized. Furthermore, the biological significance of the DNA and peptide sequence diversity, observed in the conserved motifs and domains found in the different AHL types, is a subject area that remains largely unexplored. Results Transgenic plants overexpressing AtAHL20 flowered later than the wild type under both short and long days. Transcript accumulation analyses showed that 35S:AtAHL20 plants contained reduced FT, TSF, AGL8 and SPL3 mRNA levels. Similarly, overexpression of AtAHL20’s orthologue in Camelina sativa, Arabidopsis’ closely related Brassicaceae family member species, conferred a late-flowering phenotype via suppression of CsFT expression. However, overexpression of an aberrant AtAHL20 gene harboring a missense mutation in the AT-hook domain’s highly conserved R-G-R core motif abolished the late-flowering phenotype. Data from targeted yeast-two-hybrid assays showed that AtAHL20 interacted with itself and several other Clade-A Type-I AHLs which have been previously implicated in flowering-time regulation: AtAHL19, AtAHL22 and AtAHL29. Conclusion We showed via gain-of-function analysis that AtAHL20 is a negative regulator of FT expression, as well as other downstream flowering time regulating genes. A similar outcome in Camelina sativa transgenic plants overexpressing CsAHL20 suggest that this is a conserved function. Our results demonstrate that AtAHL20 acts as a photoperiod-independent negative regulator of transition to flowering.

scholarly journals Overexpression of AtAHL20 causes delayed flowering in Arabidopsis via repression of  FT expression

2020 ◽  
Author(s):  
Reuben Tayengwa ◽  
Pushpa Sharma-Koirala ◽  
Courtney F. Pierce ◽  
Breanna E Werner ◽  
Michael M Neff
Keyword(s):  
Drought Stress ◽  
Transgenic Plants ◽  
Gene Family ◽  
Stress Tolerance ◽  
Flowering Time ◽  
Camelina Sativa ◽  
Negative Regulator ◽  
Wild Type ◽  
Late Flowering ◽  
Drought Stress Tolerance

Abstract Background The 29-member Arabidopsis AHL gene family is classified into three main classes based on nucleotide and protein sequence evolutionary differences. These differences include the presence or absence of introns, type and/or number of conserved AT-hook and PPC domains. AHL gene family members are divided into two phylogenetic clades, Clade-A and Clade-B. A majority of the 29 members remain functionally uncharacterized. Furthermore, the biological significance of the DNA and peptide sequence diversity, observed in the conserved motifs and domains found in the different AHL types, is a subject area that remains largely unexplored. Results Transgenic plants overexpressing AtAHL20 flowered later than the wild type. Transcript accumulation analyses showed that 35S:AtAHL20 plants contained reduced FT, TSF, AGL8 and SPL3 mRNA levels. Similarly, overexpression of AtAHL20’s orthologue in Camelina sativa, Arabidopsis’ closely related Brassicaceae family member species, conferred a late-flowering phenotype via suppression of CsFT expression. In addition, 35S:AtAHL20 seedlings exhibited suppressed hypocotyl length and enhanced water stress tolerance. However, overexpression of an aberrant AtAHL20 gene harboring a missense mutation in the AT-hook domain’s highly conserved R-G-R core motif abolished the late-flowering phenotype. Data from targeted yeast-two-hybrid assays showed that AtAHL20 interacted with itself and several other Clade-A Type-I AHLs which have been previously implicated in flowering-time regulation: AtAHL22, AtAHL27 and AtAHL29. Conclusion We showed via gain-function analysis that AtAHL20 is a negative regulator of FT expression, as well as other downstream flowering time regulating genes. A similar outcome in Camelina sativa transgenic plants overexpressing CsAHL20 suggest that this is a conserved function. Additionally, overexpression of AtAHL20 resulted in shorter hypocotyls and enhanced drought stress tolerance compared to wild-type plants. Our results demonstrate that AtAHL20 is a negative regulator of transition to flowering and hypocotyl elongation, but a positive regulator of drought stress tolerance.

Sign in / Sign up

Export Citation Format

Share Document