scholarly journals Targeting of the Plant Vacuolar Sorting Receptor BP80 Is Dependent on Multiple Sorting Signals in the Cytosolic Tail

2006 ◽  
Vol 18 (6) ◽  
pp. 1477-1497 ◽  
Author(s):  
Luis L.P. daSilva ◽  
Ombretta Foresti ◽  
Jurgen Denecke
Keyword(s):  
Sorting Signals ◽  
Vacuolar Sorting Receptor ◽  
Vacuolar Sorting ◽  
Sorting Receptor

scholarly journals The Plant Vacuolar Sorting Receptor Atelp Is Involved in Transport of Nh2-Terminal Propeptide-Containing Vacuolar Proteins in Arabidopsis thaliana

2000 ◽  
Vol 149 (7) ◽  
pp. 1335-1344 ◽  
Author(s):  
Sharif U. Ahmed ◽  
Enrique Rojo ◽  
Valentina Kovaleva ◽  
Sridhar Venkataraman ◽  
James E. Dombrowski ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Arabidopsis ◽  
Sorting Signals ◽  
Specific Manner ◽  
Vacuolar Sorting Receptor ◽  
Vacuolar Sorting ◽  
Sorting Receptor ◽  
Vacuolar Protein ◽  
Golgi Network ◽  
Endogenous Plant

Many soluble plant vacuolar proteins are sorted away from secreted proteins into small vesicles at the trans-Golgi network by transmembrane cargo receptors. Cleavable vacuolar sorting signals include the NH2-terminal propeptide (NTPP) present in sweet potato sporamin (Spo) and the COOH-terminal propeptide (CTPP) present in barley lectin (BL). These two proteins have been found to be transported by different mechanisms to the vacuole. We examined the ability of the vacuolar cargo receptor AtELP to interact with the sorting signals of heterologous and endogenous plant vacuolar proteins in mediating vacuolar transport in Arabidopsis thaliana. AtELP extracted from microsomes was found to interact with the NTPPs of barley aleurain and Spo, but not with the CTPPs of BL or tobacco chitinase, in a pH-dependent and sequence-specific manner. In addition, EM studies revealed the colocalization of AtELP with NTPP-Spo at the Golgi apparatus, but not with BL-CTPP in roots of transgenic Arabidopsis plants. Further, we found that AtELP interacts in a similar manner with the NTPP of the endogenous vacuolar protein AtALEU (Arabidopsis thaliana Aleu), a protein highly homologous to barley aleurain. We hypothesize that AtELP functions as a vacuolar sorting receptor involved in the targeting of NTPP-, but not CTPP-containing proteins in Arabidopsis.

scholarly journals Demonstration in Yeast of the Function of BP-80, a Putative Plant Vacuolar Sorting Receptor

2001 ◽  
Vol 13 (4) ◽  
pp. 781-792 ◽  
Author(s):  
David Humair ◽  
Doramys Hernández Felipe ◽  
Jean-Marc Neuhaus ◽  
Nadine Paris
Keyword(s):  
Vacuolar Sorting Receptor ◽  
Vacuolar Sorting ◽  
Sorting Receptor

scholarly journals Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana

2003 ◽  
Vol 100 (26) ◽  
pp. 16095-16100 ◽  
Author(s):  
T. Shimada ◽  
K. Fuji ◽  
K. Tamura ◽  
M. Kondo ◽  
M. Nishimura ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Vacuolar Sorting Receptor ◽  
Vacuolar Sorting ◽  
Sorting Receptor

scholarly journals Lack of a Vacuolar Sorting Receptor Leads to Non-Specific Missorting of Soluble Vacuolar Proteins in Arabidopsis Seeds

Traffic ◽  
2008 ◽  
Vol 9 (3) ◽  
pp. 408-416 ◽  
Author(s):  
Christian P. Craddock ◽  
Paul R. Hunter ◽  
Erika Szakacs ◽  
Giselbert Hinz ◽  
David G. Robinson ◽  
...  
Keyword(s):  
Vacuolar Sorting Receptor ◽  
Vacuolar Sorting ◽  
Sorting Receptor
Sign in / Sign up

Export Citation Format

Share Document