scholarly journals Constitutive Expression Exposes Functional Redundancy between the Arabidopsis Histone H2A Gene HTA1 and Other H2A Gene Family Members

2006 ◽  
Vol 18 (7) ◽  
pp. 1575-1589 ◽  
Author(s):  
HoChul Yi ◽  
Nagesh Sardesai ◽  
Toshinori Fujinuma ◽  
Chien-Wei Chan ◽  
Veena ◽  
...  
Keyword(s):  
Gene Family ◽  
Family Members ◽  
Constitutive Expression ◽  
Functional Redundancy ◽  
Histone H2a

scholarly journals Redundancy, feedback, and robustness in the Arabidopsis thaliana BZR/BEH gene family

2016 ◽  
Author(s):  
Jennifer Lachowiec ◽  
G. Alex Mason ◽  
Karla Schultz ◽  
Christine Queitsch
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Gene Family ◽  
Family Member ◽  
Single Gene ◽  
Family Members ◽  
Embryonic Stem ◽  
Functional Redundancy ◽  
Stem Length ◽  
Organismal Development

ABSTRACTOrganismal development is remarkably robust, tolerating stochastic errors to produce consistent, so-called canalized adult phenotypes. The mechanistic underpinnings of developmental robustness are poorly understood, but recent studies implicate certain features of genetic networks such as functional redundancy, connectivity, and feedback. Here, we examine the BRZ/BEH gene family, whose function is crucial for embryonic stem development in the plant Arabidopsis thaliana, to test current assumptions on functional redundancy and trait robustness. Our analyses of BRZ/BEH gene mutants and mutant combinations revealed that functional redundancy among gene family members does not contribute to trait robustness. Connectivity is another commonly cited determinant of robustness; however, we found no correlation between connectivity among gene family members or their connectivity with other transcription factors and effects on robustness. Instead, we found that only BEH4, the most ancient family member, modulated developmental robustness. We present evidence that regulatory cross-talk among gene family members is integrated by BEH4 and promotes wild-type levels of developmental robustness. Further, the chaperone HSP90, a known determinant of developmental robustness, appears to act via BEH4 in maintaining robustness of embryonic stem length. In summary, we demonstrate that even among closely related transcription factors, trait robustness can arise through the activity of a single gene family member, challenging common assumptions about the molecular underpinnings of robustness.

scholarly journals Isolation and Characterization of Rat Cytochrome P-450IIB Gene Family Members

1989 ◽  
Vol 264 (12) ◽  
pp. 7046-7053 ◽  
Author(s):  
C M Giachelli ◽  
J Lin-Jones ◽  
C J Omiecinski
Keyword(s):  
Gene Family ◽  
Family Members ◽  
Isolation And Characterization

scholarly journals Cloning and functional analysis of the FAD2 gene family from desert shrub Artemisia sphaerocephala

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Xiumei Miao ◽  
Lijing Zhang ◽  
Xiaowei Hu ◽  
Shuzhen Nan ◽  
Xiaolong Chen ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Gene Family ◽  
Fatty Acid Biosynthesis ◽  
Family Members ◽  
Pcr Analysis ◽  
Mrna Levels ◽  
Acid Biosynthesis ◽  
Desert Shrub ◽  
Artemisia Sphaerocephala

Abstract Background Linoleic acid is an important polyunsaturated fatty acid, required for all eukaryotes. Microsomal delta-12 (Δ12) oleate desaturase (FAD2) is a key enzyme for linoleic acid biosynthesis. Desert shrub Artemisia sphaerocephala is rich in linoleic acid, it has a large FAD2 gene family with twenty-six members. The aim of this work is to unveil the difference and potentially functionality of AsFAD2 family members. Results Full-length cDNAs of twenty-one AsFAD2 genes were obtained from A. sphaerocephala. The putative polypeptides encoded by AsFAD2 family genes showed a high level of sequence similarity and were relatively conserved during evolution. The motif composition was also relatively conservative. Quantitative real-time PCR analysis revealed that the AsFAD2–1 gene was strongly expressed in developing seeds, which may be closely associated with the high accumulating ability of linoleic acid in A. sphaerocephala seeds. Although different AsFAD2 family members showed diverse response to salt stress, the overall mRNA levels of the AsFAD2 family genes was stable. Transient expression of AsFAD2 genes in the Nicotiana benthamiana leaves revealed that the encoded proteins were all located in the endoplasmic reticulum. Heterologous expression in Saccharomyces cerevisiae suggested that only three AsFAD2 enzymes, AsFAD2–1, − 10, and − 23, were Δ12 oleate desaturases, which could convert oleic acid to linoleic acid, whereas AsFAD2–1 and AsFAD2–10 could also produce palmitolinoleic acid. Conclusions This research reported the cloning, expression studies, subcellular localization and functional identification of the large AsFAD2 gene family. These results should be helpful in understanding fatty acid biosynthesis in A. sphaerocephala, and has the potential to be applied in the study of plant fatty acids traits.

Distinguishing CPT gene family members and vetting the sequence structure of a putative rubber synthesizing variant in Hevea brasiliensis

Gene ◽  
2019 ◽  
Vol 689 ◽  
pp. 183-193
Author(s):  
Thomas Kadampanattu Uthup ◽  
Anantharamanan Rajamani ◽  
Minimol Ravindran ◽  
Thakurdas Saha
Keyword(s):  
Gene Family ◽  
Hevea Brasiliensis ◽  
Family Members ◽  
Sequence Structure

scholarly journals Functional study of CHS gene family members in citrus revealed a novel CHS gene affecting the production of flavonoids

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Zhibin Wang ◽  
Qibin Yu ◽  
Wanxia Shen ◽  
Choaa A. El Mohtar ◽  
Xiaochun Zhao ◽  
...  
Keyword(s):  
Gene Family ◽  
Family Members ◽  
Functional Study

scholarly journals Avian apolipoprotein A-V binds to LDL receptor gene family members

2007 ◽  
Vol 48 (7) ◽  
pp. 1451-1456 ◽  
Author(s):  
Andrea Dichlberger ◽  
Larry A. Cogburn ◽  
Johannes Nimpf ◽  
Wolfgang J. Schneider
Keyword(s):  
Gene Family ◽  
Receptor Gene ◽  
Family Members ◽  
Ldl Receptor ◽  
Apolipoprotein A ◽  
Receptor Gene Family

Mapping of tyrosine kinase gene family members in aXiphophorus melanoma model

1998 ◽  
Vol 22 (3) ◽  
pp. 150-157 ◽  
Author(s):  
Donald C. Morizot ◽  
Brenda B. McEntire ◽  
Luis Della Coletta ◽  
Steven Kazianis ◽  
Manfred Schartl ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
Gene Family ◽  
Family Members ◽  
Kinase Gene ◽  
Melanoma Model ◽  
Tyrosine Kinase Gene
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