scholarly journals PIL5, a Phytochrome-Interacting Basic Helix-Loop-Helix Protein, Is a Key Negative Regulator of Seed Germination in Arabidopsis thaliana

2004 ◽  
Vol 16 (11) ◽  
pp. 3045-3058 ◽  
Author(s):  
Eunkyoo Oh ◽  
Jonghyun Kim ◽  
Eunae Park ◽  
Jeong-Il Kim ◽  
Changwon Kang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Seed Germination ◽  
Negative Regulator ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix

scholarly journals A Dual Mechanism Controls Nuclear Localization in the Atypical Basic-Helix-Loop-Helix Protein PAR1 of Arabidopsis thaliana

2012 ◽  
Vol 5 (3) ◽  
pp. 669-677 ◽  
Author(s):  
Anahit Galstyan ◽  
Jordi Bou-Torrent ◽  
Irma Roig-Villanova ◽  
Jaime F. Martínez-García
Keyword(s):  
Arabidopsis Thaliana ◽  
Nuclear Localization ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix ◽  
Dual Mechanism

scholarly journals FoSTUA, Encoding a Basic Helix-Loop-Helix Protein, Differentially Regulates Development of Three Kinds of Asexual Spores, Macroconidia, Microconidia, and Chlamydospores, in the Fungal Plant Pathogen Fusarium oxysporum

2004 ◽  
Vol 3 (6) ◽  
pp. 1412-1422 ◽  
Author(s):  
Toshiaki Ohara ◽  
Takashi Tsuge
Keyword(s):  
Fusarium Oxysporum ◽  
Fluorescent Protein ◽  
Negative Regulator ◽  
Vascular Wilt ◽  
Wild Type ◽  
Low Frequencies ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix ◽  
Chlamydospore Formation ◽  
Green Fluorescent

ABSTRACT The soil-borne fungus Fusarium oxysporum causes vascular wilt of a wide variety of plant species. F. oxysporum produces three kinds of asexual spores, macroconidia, microconidia, and chlamydospores. Falcate macroconidia are formed generally from terminal phialides on conidiophores and rarely from intercalary phialides on hyphae. Ellipsoidal microconidia are formed from intercalary phialides on hyphae. Globose chlamydospores with thick walls are developed by the modification of hyphal and conidial cells. Here we describe FoSTUA of F. oxysporum, which differentially regulates the development of macroconidia, microconidia, and chlamydospores. FoSTUA encodes a basic helix-loop-helix protein with similarity to Aspergillus nidulans StuA, which has been identified as a transcriptional regulator controlling conidiation. Nuclear localization of FoStuA was verified by using strains expressing FoStuA-green fluorescent protein fusions. The FoSTUA-targeted mutants exhibited normal microconidium formation in cultures. However, the mutants lacked conidiophores and produced macroconidia at low frequencies only from intercalary phialides. Thus, FoSTUA appears to be necessary to induce conidiophore differentiation. In contrast, chlamydospore formation was dramatically promoted in the mutants. These data demonstrate that FoStuA is a positive regulator and a negative regulator for the development of macroconidia and chlamydospores, respectively, and is dispensable for microconidium formation in cultures. The disease-causing ability of F. oxysporum was not affected by mutations in FoSTUA. However, the mutants produced markedly fewer macroconidia and microconidia in infected plants than the wild type. These results suggest that FoSTUA also has an important role for microconidium formation specifically in infected plants.

scholarly journals The Basic Helix-Loop-Helix Transcription Factor SmbHLH1 Represses Anthocyanin Biosynthesis in Eggplant

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhaofei Duan ◽  
Shiyu Tian ◽  
Guobin Yang ◽  
Min Wei ◽  
Jing Li ◽  
...  
Keyword(s):  
Plant Species ◽  
Anthocyanin Biosynthesis ◽  
Solanum Melongena ◽  
Amino Acid Sequences ◽  
Negative Regulator ◽  
Bimolecular Fluorescence Complementation ◽  
Luciferase Assay ◽  
Anthocyanin Accumulation ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix

Many basic helix-loop-helix transcription factors (TFs) have been reported to promote anthocyanin biosynthesis in numerous plant species, but little is known about bHLH TFs that inhibit anthocyanin accumulation. In this study, SmbHLH1 from Solanum melongena was identified as a negative regulator of anthocyanin biosynthesis. However, SmbHLH1 showed high identity with SmTT8, which acts as a SmMYB113-dependent positive regulator of anthocyanin-biosynthesis in plants. Overexpression of SmbHLH1 in eggplant caused a dramatic decrease in anthocyanin accumulation. Only the amino acid sequences at the N and C termini of SmbHLH1 differed from the SmTT8 sequence. Expression analysis revealed that the expression pattern of SmbHLH1 was opposite to that of anthocyanin accumulation. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that SmbHLH1 could not interact with SmMYB113. Dual-luciferase assay demonstrated that SmbHLH1 directly repressed the expression of SmDFR and SmANS. Our results demonstrate that the biological function of bHLHs in anthocyanin biosynthesis may have evolved and provide new insight into the molecular functions of orthologous genes from different plant species.

Myostatin silencing effect on basic helix–loop–helix transcription factors in caprine foetal fibroblast cells

2017 ◽  
Author(s):  
Biswajyoti Borah ◽  
Ajit Pratap Singh ◽  
Hamen Gogoi ◽  
Amlan Jyoti Phukan ◽  
Bikash Chandra Sarkhel
Keyword(s):  
Transcription Factors ◽  
Muscle Development ◽  
Pearson Correlation ◽  
Negative Regulator ◽  
Fibroblast Cells ◽  
Animal Cells ◽  
Myostatin Gene ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix

Transgenic food animal production is one of the potential and need oriented research to mitigate the food crises of the world. In vitro gene silenced animal cells and making use of these cells for transgenesis one of the suitable way to produce productive animals. Myostatin is a negative regulator of muscle growth, has the potential to increase the muscle mass upon its silencing. Four Hush 29-mer anti- myostatin (MSTN) shRNA constructs were checked for myostatin gene silencing in caprine foetal fibroblast cells and its subsequent effect on basic helix– loop–helix (bHLH) transcription factors. These factors are necessary for the terminal differentiation, proliferation, and homeostasis of muscle development. Different shRNA constructs displayed 55.1 to 91.5% (p less than 0.01) of myostatin silencing in caprine foetal fibroblast cells and upregulation of myogenic gene. Upregulation of 7.97 to 111.67 % for MyoD, 77.0 % to 319.47 % for myogenin, 16.67 % to 138.0 % for Myf5 were observed . The Pearson correlation established a negative correlation between myostatin and genes under study. Result suggests that knockdown of MSTN a potential approach to improve caprine musculatures.

scholarly journals Overexpression of myogenin in muscles of transgenic mice: interaction with Id-1, negative crossregulation of myogenic factors, and induction of extrasynaptic acetylcholine receptor expression.

1995 ◽  
Vol 15 (12) ◽  
pp. 7127-7134 ◽  
Author(s):  
K Gundersen ◽  
I Rabben ◽  
B J Klocke ◽  
J P Merlie
Keyword(s):  
Transgenic Mice ◽  
Neonatal Mortality ◽  
Acetylcholine Receptor ◽  
Muscle Fibers ◽  
Receptor Expression ◽  
Negative Regulator ◽  
Mrna Levels ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix ◽  
Myogenic Factors

To investigate the role of myogenin in regulating acetylcholine receptor expression in adult muscle, this muscle-specific basic helix-loop-helix transcription factor was overexpressed in transgenic mice by using regulatory elements conferring strong expression confined to differentiated postmitotic muscle fibers. Many of the transgenic mice died during the first postnatal week, but those that survived into adulthood displayed normal muscle histology, gross morphology, and motor behavior. The mRNA levels of all five acetylcholine receptor subunits (alpha, beta, gamma, delta, and epsilon) were, however, elevated. Also, the level of receptor protein was increased and high levels of receptors were present throughout the extrasynaptic surface membrane of the muscle fibers. Thus, elevated levels of myogenin are apparently sufficient to induce acetylcholine supersensitivity in normally innervated muscle of adult mice. The high neonatal mortality rate of the mice overexpressing myogenin hindered the propagation of a stable line. In an attempt to increase survival, myogenin overexpressers were mated with a line of transgenic mice overexpressing Id-1, a negative regulator that interacts with the basic helix-loop-helix family of transcription factors. The Id-1 transgene apparently worked as a second site suppressor and abolished the high rate of neonatal mortality. This effect indicates that Id-1 and myogenin interact directly or indirectly in these animals. Further study indicated that myogenin overexpression had no effect on the level of endogenous myogenin mRNA, while the levels of myoD and MRF4 mRNAs were reduced. Overexpression of the negative regulator Id-1 increased the mRNA levels of all the myogenic factors. These findings are consistent with a hypothesis suggesting that myogenic factors are influenced by mechanisms that maintain cellular homeostasis.

The basic helix-loop-helix transcription factor, bHLH11 functions in the iron-uptake system in Arabidopsis thaliana

2018 ◽  
Vol 132 (1) ◽  
pp. 93-105 ◽  
Author(s):  
Noriaki Tanabe ◽  
Masahiro Noshi ◽  
Daisuke Mori ◽  
Kotaro Nozawa ◽  
Masahiro Tamoi ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Iron Uptake ◽  
Uptake System ◽  
Basic Helix Loop Helix ◽  
Iron Uptake System ◽  
Helix Loop Helix

scholarly journals Update on the Basic Helix-Loop-Helix Transcription Factor Gene Family in Arabidopsis thaliana

2003 ◽  
Vol 15 (11) ◽  
pp. 2497-2502 ◽  
Author(s):  
Paul C. Bailey ◽  
Cathie Martin ◽  
Gabriela Toledo-Ortiz ◽  
Peter H. Quail ◽  
Enamul Huq ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Gene Family ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix ◽  
Transcription Factor Gene Family
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