scholarly journals Genetic Interaction of an Origin Recognition Complex Subunit and the Polycomb Group Gene MEDEA during Seed Development

2004 ◽  
Vol 16 (4) ◽  
pp. 1035-1046 ◽  
Author(s):  
Margaret A. Collinge ◽  
Charles Spillane ◽  
Claudia Köhler ◽  
Jacqueline Gheyselinck ◽  
Ueli Grossniklaus
Keyword(s):  
Seed Development ◽  
Origin Recognition Complex ◽  
Genetic Interaction ◽  
Polycomb Group ◽  
Polycomb Group Gene ◽  
Origin Recognition

scholarly journals Homeotic transformations of the axial skeleton of YY1 mutant mice and genetic interaction with the Polycomb group gene Ring1/Ring1A

2006 ◽  
Vol 123 (4) ◽  
pp. 312-320 ◽  
Author(s):  
Mar Lorente ◽  
Claudia Pérez ◽  
Carmen Sánchez ◽  
Mary Donohoe ◽  
Yang Shi ◽  
...  
Keyword(s):  
Genetic Interaction ◽  
Axial Skeleton ◽  
Polycomb Group ◽  
Mutant Mice ◽  
Polycomb Group Gene

scholarly journals CDC45, a novel yeast gene that functions with the origin recognition complex and Mcm proteins in initiation of DNA replication.

1997 ◽  
Vol 17 (2) ◽  
pp. 553-563 ◽  
Author(s):  
L Zou ◽  
J Mitchell ◽  
B Stillman
Keyword(s):  
Dna Replication ◽  
Origin Recognition Complex ◽  
Genetic Interaction ◽  
Sequence Similarity ◽  
Replication Initiation ◽  
Nonpermissive Temperature ◽  
Functional Connection ◽  
Initiation Of Dna Replication ◽  
Mcm Proteins ◽  
Origin Recognition

The CDC45 gene of Saccharomyces cerevisiae was isolated by complementation of the cold-sensitive cdc45-1 mutant and shown to be essential for cell viability. Although CDC45 genetically interacts with a group of MCM genes (CDC46, CDC47, and CDC54), the predicted sequence of its protein product reveals no significant sequence similarity to any known Mcm family member. Further genetic characterization of the cdc45-1 mutant demonstrated that it is synthetically lethal with orc2-1, mcm2-1, and mcm3-1. These results not only reveal a functional connection between the origin recognition complex (ORC) and Cdc45p but also extend the CDC45-MCM genetic interaction to all known MCM family members that were shown to be involved in replication initiation. Initiation of DNA replication in cdc45-1 cells was defective, causing a delayed entry into S phase at the nonpermissive temperature, as well as a high plasmid loss rate which could be suppressed by tandem copies of replication origins. Furthermore, two-dimensional gels directly showed that chromosomal origins fired less frequently in cdc45-1 cells at the nonpermissive temperature. These findings suggest that Cdc45p, ORC, and Mcm proteins act in concert for replication initiation throughout the genome.

Polycomb group gene function in sexual and asexual seed development in angiosperms

2009 ◽  
Vol 23 (2) ◽  
pp. 123-133 ◽  
Author(s):  
Julio C. M. Rodrigues ◽  
Ming Luo ◽  
Frédéric Berger ◽  
Anna M. G. Koltunow
Keyword(s):  
Seed Development ◽  
Gene Function ◽  
Polycomb Group ◽  
Polycomb Group Gene

scholarly journals Polycomb Group Gene OsFIE2 Regulates Rice (Oryza sativa) Seed Development and Grain Filling via a Mechanism Distinct from Arabidopsis

PLoS Genetics ◽  
2013 ◽  
Vol 9 (3) ◽  
pp. e1003322 ◽  
Author(s):  
Babi Ramesh Reddy Nallamilli ◽  
Jian Zhang ◽  
Hana Mujahid ◽  
Brandon M. Malone ◽  
Susan M. Bridges ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Seed Development ◽  
Grain Filling ◽  
Polycomb Group ◽  
Polycomb Group Gene

The metazoan origin recognition complex

1999 ◽  
Vol 4 (1-3) ◽  
pp. d805 ◽  
Author(s):  
David, G. Quintana
Keyword(s):  
Origin Recognition Complex ◽  
Origin Recognition

scholarly journals Humanizing the yeast origin recognition complex

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Clare S. K. Lee ◽  
Ming Fung Cheung ◽  
Jinsen Li ◽  
Yongqian Zhao ◽  
Wai Hei Lam ◽  
...  
Keyword(s):  
Amino Acid ◽  
Protein Complex ◽  
Origin Recognition Complex ◽  
Specific Binding ◽  
Life Cycles ◽  
Amino Acid Insertion ◽  
Evolutionarily Conserved ◽  
Transcriptional Start Sites ◽  
Eukaryote Genome ◽  
Origin Recognition

AbstractThe Origin Recognition Complex (ORC) is an evolutionarily conserved six-subunit protein complex that binds specific sites at many locations to coordinately replicate the entire eukaryote genome. Though highly conserved in structure, ORC’s selectivity for replication origins has diverged tremendously between yeasts and humans to adapt to vastly different life cycles. In this work, we demonstrate that the selectivity determinant of ORC for DNA binding lies in a 19-amino acid insertion helix in the Orc4 subunit, which is present in yeast but absent in human. Removal of this motif from Orc4 transforms the yeast ORC, which selects origins based on base-specific binding at defined locations, into one whose selectivity is dictated by chromatin landscape and afforded with plasticity, as reported for human. Notably, the altered yeast ORC has acquired an affinity for regions near transcriptional start sites (TSSs), which the human ORC also favors.

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