scholarly journals Regulation of Root Hair Initiation and Expansin Gene Expression in Arabidopsis

2002 ◽  
Vol 14 (12) ◽  
pp. 3237-3253 ◽  
Author(s):  
Hyung-Taeg Cho ◽  
Daniel J. Cosgrove
Keyword(s):  
Gene Expression ◽  
Root Hair ◽  
Expansin Gene ◽  
Root Hair Initiation

Ultrastructure of Medicago sativa rhizodermis cells over their early development

Biologia ◽  
2008 ◽  
Vol 63 (2) ◽  
Author(s):  
Lucia Mikolajová ◽  
Halina Vargová ◽  
Zora Hanáčková ◽  
Milada Čiamporová
Keyword(s):  
Cell Wall ◽  
Medicago Sativa ◽  
Root Hair ◽  
Phosphotungstic Acid ◽  
Root Tip ◽  
Cell Wall Polysaccharides ◽  
Golgi Bodies ◽  
Internalization Process ◽  
Subcellular Level ◽  
Root Hair Initiation

AbstractUltrastructure was investigated along the files of developing epidermal cells in the root tip of a model plant Medicago sativa, in which all rhizodermal cells are potential hair-forming trichoblasts. Differentiation at subcellular level was observed up to the stage of bulge initiation in the trichoblasts. Root hair initiation indicated by the emergence of bulges from trichoblasts was detected at various distances from the root tip and, it was independent of the trichoblast size.During rhizodermal cell differentiation, starch grains accumulated in the plastids. Nuclei located in the central part of the young, meristematic cells moved towards the inner periclinal wall as the central vacuole enlarged. The bulging region of the trichoblasts located opposite the nucleus and was rich in mitochondria, ER, ribosomes, and Golgi bodies, and contained also vesicles enclosing fibrillar material. This material responded positively to phosphotungstic acid, which was used for detection of cell wall polysaccharides. The cell wall thickness within the bulging domain was significantly lower than in other parts of trichoblasts. We suggest that internalization of cell wall polysaccharides occurs within the bulging area, contributing to local thinning of the cell wall and providing a source of osmotically active compounds for maintaining turgor in the trichoblast. Thus, the internalization process might be necessary for root hair outgrowth.

scholarly journals Alternative Oxidase Inhibition Impairs Tobacco Root Development and Root Hair Formation

2021 ◽  
Vol 12 ◽  
Author(s):  
Yang Liu ◽  
Lu-Lu Yu ◽  
Ye Peng ◽  
Xin-Xin Geng ◽  
Fei Xu
Keyword(s):  
Gene Expression ◽  
Root Development ◽  
Transcriptome Analysis ◽  
Root Hair ◽  
Alternative Oxidase ◽  
Regulation Of Gene Expression ◽  
Main Root ◽  
Respiratory Pathway ◽  
Root Hair Formation ◽  
Hair Formation

Alternative oxidase (AOX) is the terminal oxidase of the mitochondrial respiratory electron transport chain in plant cells and is critical for the balance of mitochondrial hemostasis. In this study, the effect of inhibition of AOX with different concentrations of salicylhydroxamic acid (SHAM) on the tobacco root development was investigated. We show here that AOX inhibition significantly impaired the development of the main root and root hair formation of tobacco. The length of the main root of SHAM-treated tobacco was significantly shorter than that of the control, and no root hairs were formed after treatment with a concentration of 1 mM SHAM or more. The transcriptome analysis showed that AOX inhibition by 1 mM SHAM involved in the regulation of gene expression related to root architecture. A total of 5,855 differentially expressed genes (DEGs) were obtained by comparing SHAM-treated roots with control. Of these, the gene expression related to auxin biosynthesis and perception were significantly downregulated by 1 mM SHAM. Similarly, genes related to cell wall loosening, cell cycle, and root meristem growth factor 1 (RGF1) also showed downregulation on SHAM treatment. Moreover, combined with the results of physiological measurements, the transcriptome analysis demonstrated that AOX inhibition resulted in excessive accumulation of reactive oxygen species in roots, which further induced oxidative damage and cell apoptosis. It is worth noting that when indoleacetic acid (20 nM) and dimethylthiourea (10 mM) were added to the medium containing SHAM, the defects of tobacco root development were alleviated, but to a limited extent. Together, these findings indicated that AOX-mediated respiratory pathway plays a crucial role in the tobacco root development, including root hair formation.

scholarly journals Expansin gene expression and anoxic coleoptile elongation in rice cultivars

2009 ◽  
Vol 166 (14) ◽  
pp. 1576-1580 ◽  
Author(s):  
Leonardo Magneschi ◽  
Rasika Lasanthi Kudahettige ◽  
Amedeo Alpi ◽  
Pierdomenico Perata
Keyword(s):  
Gene Expression ◽  
Rice Cultivars ◽  
Expansin Gene ◽  
Coleoptile Elongation

scholarly journals Spot Dynamics in a Reaction-Diffusion Model of Plant Root Hair Initiation

2018 ◽  
Vol 78 (1) ◽  
pp. 291-319 ◽  
Author(s):  
Daniele Avitabile ◽  
Victor F. Bren͂a ◽  
Michael J. Ward
Keyword(s):  
Diffusion Model ◽  
Root Hair ◽  
Plant Root ◽  
Reaction Diffusion ◽  
Reaction Diffusion Model ◽  
Root Hair Initiation

scholarly journals Nod Factor-Induced Expression of Leghemoglobin to Study the Mechanism of NH4NO3 Inhibition on Root Hair Deformation

1997 ◽  
Vol 10 (2) ◽  
pp. 215-220 ◽  
Author(s):  
Renze Heidstra ◽  
Gerd Nilsen ◽  
Francisco Martinez-Abarca ◽  
Ab van Kammen ◽  
Ton Bisseling
Keyword(s):  
Gene Expression ◽  
Root Hair ◽  
Rhizobium Leguminosarum ◽  
Cortical Cell ◽  
Brefeldin A ◽  
Marker Genes ◽  
Nodule Formation ◽  
Cdna Clones ◽  
Nod Factor ◽  
Root Hair Deformation

Nod factors secreted by Rhizobium leguminosarum bv. viciae induce root hair deformation, the formation of nodule primordia, and the expression of early nodulin genes in Vicia sativa (vetch). Root hair deformation is induced within 3 h in a small, susceptible zone (±2 mm) of the root. NH4NO3, known to be a potent blocker of nodule formation, inhibits root hair deformation, initial cortical cell divisions, and infection thread formation. To test whether NH4NO3 affects the formation of a component of the Nod factor perception-transduction system, we studied Nod factor-induced gene expression. The differential display technique was used to search for marker genes, which are induced within 1 to 3 h after Nod factor application. Surprisingly, one of the isolated cDNA clones was identified as a leghemoglobin gene (VsLb1), which is induced in vetch roots within 1 h after Nod factor application. By using the drug brefeldin A, it was then shown that VsLb1 activation does not require root hair deformation. The pVsLb1 clone was used as a marker to show that in vetch plants grown in the presence of NH4NO3 Nod factor perception and transduction leading to gene expression are unaffected.

Mathematical Modeling of Plant Root Hair Initiation: Dynamics of Localized Patches

2014 ◽  
Vol 13 (1) ◽  
pp. 210-248 ◽  
Author(s):  
V. Bren͂a--Medina ◽  
A. R. Champneys ◽  
C. Grierson ◽  
M. J. Ward
Keyword(s):  
Mathematical Modeling ◽  
Root Hair ◽  
Plant Root ◽  
Root Hair Initiation
Sign in / Sign up

Export Citation Format

Share Document