scholarly journals A Gain-of-Function Mutation in an Arabidopsis Toll Interleukin1 Receptor–Nucleotide Binding Site–Leucine-Rich Repeat Type R Gene Triggers Defense Responses and Results in Enhanced Disease Resistance

2002 ◽  
Vol 14 (12) ◽  
pp. 3149-3162 ◽  
Author(s):  
Yumiko Shirano ◽  
Pradeep Kachroo ◽  
Jyoti Shah ◽  
Daniel F. Klessig
Keyword(s):  
Disease Resistance ◽  
Binding Site ◽  
Nucleotide Binding ◽  
Defense Responses ◽  
Nucleotide Binding Site ◽  
R Gene ◽  
Leucine Rich Repeat ◽  
Repeat Type ◽  
Gain Of Function

Genome-wide identification and characterization of nucleotide binding site leucine-rich repeat genes in linseed reveal distinct patterns of gene structure

Genome ◽  
2013 ◽  
Vol 56 (2) ◽  
pp. 91-99 ◽  
Author(s):  
Sandip M. Kale ◽  
Varsha C. Pardeshi ◽  
Vitthal T. Barvkar ◽  
Vidya S. Gupta ◽  
Narendra Y. Kadoo
Keyword(s):  
Disease Resistance ◽  
Binding Site ◽  
Regulatory Element ◽  
Nucleotide Binding ◽  
Defense Responses ◽  
Nucleotide Binding Site ◽  
Leucine Rich Repeat ◽  
Quantitative Expression ◽  
Domain Arrangement ◽  
Gene Structures

Plants employ different disease-resistance genes to detect pathogens and to induce defense responses. The largest class of these genes encodes proteins with nucleotide binding site (NBS) and leucine-rich repeat (LRR) domains. To identify the putative NBS–LRR encoding genes from linseed, we analyzed the recently published linseed genome sequence and identified 147 NBS–LRR genes. The NBS domain was used for phylogeny construction and these genes were classified into two well-known families, non-TIR (CNL) and TIR related (TNL), and formed eight clades in the neighbor-joining bootstrap tree. Eight different gene structures were observed among these genes. An unusual domain arrangement was observed in the TNL family members, predominantly in the TNL-5 clade members belonging to class D. About 12% of the genes observed were linseed specific. The study indicated that the linseed genes probably have an ancient origin with few progenitor genes. Quantitative expression analysis of five genes showed inducible expression. The in silico expression evidence was obtained for a few of these genes, and the expression was not correlated with the presence of any particular regulatory element or with unusual domain arrangement in those genes. This study will help in understanding the evolution of these genes, the development of disease resistant varieties, and the mechanism of disease resistance in linseed.

A superfamily of disease resistance gene analogs is located on all homoeologous chromosome groups of wheat (Triticum aestivum)

Genome ◽  
1998 ◽  
Vol 41 (6) ◽  
pp. 782-788 ◽  
Author(s):  
W Spielmeyer ◽  
M Robertson ◽  
N Collins ◽  
D Leister ◽  
P Schulze-Lefert ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Binding Site ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Resistance Gene Analogs ◽  
Leucine Rich Repeat ◽  
Homoeologous Chromosome ◽  
Entry Points

In this study, resistance gene analogs (RGAs) which were isolated from monocot crop species (wheat, barley, maize and rice) and contained conserved sequence motifs found within the nucleotide binding site - leucine rich repeat (NBS-LRR) class of resistance genes, were used to assess their distribution in the wheat genome. The RGAs showed 30-70% amino acid identity to a previously isolated monocot NBS-LRR sequence from the Cre3 locus for cereal cyst nematode (CCN) resistance in wheat. We used the RGAs as probes to identify and map loci in wheat using recombinant inbred lines of an international Triticeae mapping family. RGA loci mapped across all seven homoeologous chromosome groups of wheat. This study demonstrated that the RGA mapping approach provides potential entry points toward identifying resistance gene candidates in wheat.Key words: wheat, disease resistance genes, nucleotide binding site, leucine rich repeat, resistance gene analogs.

scholarly journals Comparative Genetics of Nucleotide Binding Site-Leucine Rich Repeat Resistance Gene Homologues in the Genomes of Two Dicotyledons: Tomato and Arabidopsis

Genetics ◽  
2000 ◽  
Vol 155 (1) ◽  
pp. 309-322 ◽  
Author(s):  
Qilin Pan ◽  
Yong-Sheng Liu ◽  
Ofra Budai-Hadrian ◽  
Marianne Sela ◽  
Lea Carmel-Goren ◽  
...  
Keyword(s):  
Binding Site ◽  
Plant Disease Resistance ◽  
Rapid Evolution ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Genetic Maps ◽  
R Gene ◽  
Leucine Rich Repeat ◽  
Plant Families ◽  
Plant Pathogen Interactions

Abstract The presence of a single resistance (R) gene allele can determine plant disease resistance. The protein products of such genes may act as receptors that specifically interact with pathogen-derived factors. Most functionally defined R-genes are of the nucleotide binding site-leucine rich repeat (NBS-LRR) supergene family and are present as large multigene families. The specificity of R-gene interactions together with the robustness of plant-pathogen interactions raises the question of their gene number and diversity in the genome. Genomic sequences from tomato showing significant homology to genes conferring race-specific resistance to pathogens were identified by systematically “scanning” the genome using a variety of primer pairs based on ubiquitous NBS motifs. Over 70 sequences were isolated and 10% are putative pseudogenes. Mapping of the amplified sequences on the tomato genetic map revealed their organization as mixed clusters of R-gene homologues that showed in many cases linkage to genetically characterized tomato resistance loci. Interspecific examination within Lycopersicon showed the existence of a null allele. Consideration of the tomato and potato comparative genetic maps unveiled conserved syntenic positions of R-gene homologues. Phylogenetic clustering of R-gene homologues within tomato and other Solanaceae family members was observed but not with R-gene homologues from Arabidopsis thaliana. Our data indicate remarkably rapid evolution of R-gene homologues during diversification of plant families.

scholarly journals Plant Nucleotide Binding Site–Leucine-Rich Repeat (NBS-LRR) Genes: Active Guardians in Host Defense Responses

2013 ◽  
Vol 14 (4) ◽  
pp. 7302-7326 ◽  
Author(s):  
Daniela Marone ◽  
Maria Russo ◽  
Giovanni Laidò ◽  
Anna De Leonardis ◽  
Anna Mastrangelo
Keyword(s):  
Binding Site ◽  
Host Defense ◽  
Nucleotide Binding ◽  
Defense Responses ◽  
Nucleotide Binding Site ◽  
Leucine Rich Repeat
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