scholarly journals Cytoplasmic Acidification Induced by Inorganic Phosphate Uptake in Suspension Cultured Catharanthus roseus Cells

1992 ◽  
Vol 99 (2) ◽  
pp. 672-680 ◽  
Author(s):  
Katsuhiro Sakano ◽  
Yoshiaki Yazaki ◽  
Tetsuro Mimura
Keyword(s):  
Catharanthus Roseus ◽  
Inorganic Phosphate ◽  
Phosphate Uptake ◽  
Cytoplasmic Acidification

Physiological control of phosphate uptake and phosphate homeostasis in plant cells

2001 ◽  
Vol 28 (7) ◽  
pp. 655 ◽  
Author(s):  
Tetsuro Mimura
Keyword(s):  
Plasma Membrane ◽  
Driving Force ◽  
Inorganic Phosphate ◽  
Phosphate Uptake ◽  
Plant Cells ◽  
Phosphate Homeostasis ◽  
Physiological Control ◽  
Physiological Level ◽  
Pi Transport ◽  
Cytoplasmic Acidification

Inorganic phosphate (Pi) uptake systems across the plasma membrane of plant cells have been extensively investigated. Physiological studies have established that Pi is transported into plant cells via co-transport with H+ , and in some plants with Na + , using the driving force provided by the electrogenic H + pump in the plasma membrane. Molecular studies have identified many genes for Pi transporters and are providing insights into the mechanisms of genetic control of Pi transport. There still remain, however, questions as to how Pi uptake systems are regulated at the physiological level. We have found that Pi uptake induces cytoplasmic acidification, and, conversely, that inducing cytoplasmic acidification causes the cytoplasmic Pi concentration to decrease. Both of these responses affect the operation of the H + -pump. These phenomena are discussed in relation to a possible mechanism for the physiological control of Pi uptake by plant cells.

Inorganic phosphate uptake in intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don under varying Pi status

Planta ◽  
2006 ◽  
Vol 225 (3) ◽  
pp. 711-718 ◽  
Author(s):  
Miwa Ohnishi ◽  
Tetsuro Mimura ◽  
Tomoko Tsujimura ◽  
Naoto Mitsuhashi ◽  
Setsuko Washitani-Nemoto ◽  
...  
Keyword(s):  
Catharanthus Roseus ◽  
Inorganic Phosphate ◽  
Cultured Cells ◽  
Phosphate Uptake ◽  
Suspension Cultured Cells

Inorganic phosphate uptake in Trypanosoma cruzi is coupled to K+ cycling and to active Na+ extrusion

2013 ◽  
Vol 1830 (8) ◽  
pp. 4265-4273 ◽  
Author(s):  
C.F. Dick ◽  
A.L.A. Dos-Santos ◽  
D. Majerowicz ◽  
L.S. Paes ◽  
N.L. Giarola ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Inorganic Phosphate ◽  
Phosphate Uptake

The Kinetics of Inorganic Phosphate Uptake and Utilization by Chick Heart Mitochondria

1988 ◽  
pp. 313-323
Author(s):  
Peter P. Toth ◽  
Britton Chance ◽  
John E. Sell ◽  
John F. Holland ◽  
Shelagh Ferguson-Miller ◽  
...  
Keyword(s):  
Inorganic Phosphate ◽  
Phosphate Uptake ◽  
Heart Mitochondria ◽  
Chick Heart ◽  
Kinetics Of

scholarly journals Maize defective kernel5 is a bacterial TamB homologue required for chloroplast envelope biogenesis

2019 ◽  
Vol 218 (8) ◽  
pp. 2638-2658 ◽  
Author(s):  
Junya Zhang ◽  
Shan Wu ◽  
Susan K. Boehlein ◽  
Donald R. McCarty ◽  
Gaoyuan Song ◽  
...  
Keyword(s):  
Inorganic Phosphate ◽  
Phosphate Uptake ◽  
Soluble Sugars ◽  
Chloroplast Envelope ◽  
Envelope Membrane ◽  
Double Membrane ◽  
Plastid Envelope ◽  
Membrane Envelope ◽  
Bacterial Outer Membrane ◽  
Outer Membrane Biogenesis

Chloroplasts are of prokaryotic origin with a double-membrane envelope separating plastid metabolism from the cytosol. Envelope membrane proteins integrate chloroplasts with the cell, but envelope biogenesis mechanisms remain elusive. We show that maize defective kernel5 (dek5) is critical for envelope biogenesis. Amyloplasts and chloroplasts are larger and reduced in number in dek5 with multiple ultrastructural defects. The DEK5 protein is homologous to rice SSG4, Arabidopsis thaliana EMB2410/TIC236, and Escherichia coli tamB. TamB functions in bacterial outer membrane biogenesis. DEK5 is localized to the envelope with a topology analogous to TamB. Increased levels of soluble sugars in dek5 developing endosperm and elevated osmotic pressure in mutant leaf cells suggest defective intracellular solute transport. Proteomics and antibody-based analyses show dek5 reduces levels of Toc75 and chloroplast envelope transporters. Moreover, dek5 chloroplasts reduce inorganic phosphate uptake with at least an 80% reduction relative to normal chloroplasts. These data suggest that DEK5 functions in plastid envelope biogenesis to enable transport of metabolites and proteins.

Phosphate uptake across the tonoplast of intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don

Planta ◽  
2000 ◽  
Vol 211 (3) ◽  
pp. 390-395 ◽  
Author(s):  
Agnès Massonneau ◽  
Enrico Martinoia ◽  
Karl-Josef Dietz ◽  
Tetsuro Mimura
Keyword(s):  
Catharanthus Roseus ◽  
Cultured Cells ◽  
Phosphate Uptake ◽  
Suspension Cultured Cells

Characterization of Phosphate Uptake by Suspension Cultured Catharanthus roseus Cells

1992 ◽  
Vol 140 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Maria-Elisabeth Schmidt ◽  
Sabina Heim ◽  
Claudia Wylegalla ◽  
Claudia Helmbrecht ◽  
Karl G. Wagner
Keyword(s):  
Catharanthus Roseus ◽  
Phosphate Uptake

Phosphate Uptake into Organic Compounds in Skeletal Muscle

1975 ◽  
Vol 53 (2) ◽  
pp. 317-321
Author(s):  
C. R. Dunkley ◽  
J. F. Manery
Keyword(s):  
Skeletal Muscle ◽  
Organic Compounds ◽  
Adenosine Triphosphate ◽  
Inorganic Phosphate ◽  
Phosphate Uptake ◽  
Organic Phosphate ◽  
Glycerol Phosphate ◽  
Water Extracts ◽  
Ringer’S Solution ◽  
Phosphate Compounds

Isolated intact frog muscles were incubated in 32P-labelled Ringer's solution for various periods of time (30 s – 20 h). Labelled compounds were isolated from TCA, methanol–chloroform–water, and water extracts of muscle. Hexosephosphates, phosphocreatine, phosphoenolphyruvate, α-glycerol phosphate, adenosine triphosphate, and inorganic phosphate were identified after 30 s, and 4 h incubation. Much more labelling was found after 20 h. The incorporation of 32P in 30 s into organic phosphate compounds, such as α-glycerol phosphate and ATP, showed that immediate esterification of Pi occurred on, or just inside, the sarcolemma.

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