scholarly journals Characterization of Fatty Acid Biosynthesis in Isolated Pea Root Plastids

1991 ◽  
Vol 96 (2) ◽  
pp. 602-608 ◽  
Author(s):  
Richard J. Stahl ◽  
Salvatore A. Sparace
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Acid Biosynthesis ◽  
Pea Root

scholarly journals Structural and dynamical rationale for fatty acid unsaturation inEscherichia coli

2019 ◽  
Vol 116 (14) ◽  
pp. 6775-6783 ◽  
Author(s):  
Greg J. Dodge ◽  
Ashay Patel ◽  
Kara L. Jaremko ◽  
J. Andrew McCammon ◽  
Janet L. Smith ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Active Sites ◽  
Fatty Acid Biosynthesis ◽  
Binding Pocket ◽  
Cross Linking ◽  
Substrate Selectivity ◽  
Acid Biosynthesis ◽  
Acyl Carrier Proteins ◽  
Dynamics Simulations

Fatty acid biosynthesis in α- and γ-proteobacteria requires two functionally distinct dehydratases, FabA and FabZ. Here, mechanistic cross-linking facilitates the structural characterization of a stable hexameric complex of sixEscherichia coliFabZ dehydratase subunits with six AcpP acyl carrier proteins. The crystal structure sheds light on the divergent substrate selectivity of FabA and FabZ by revealing distinct architectures of the binding pocket. Molecular dynamics simulations demonstrate differential biasing of substrate orientations and conformations within the active sites of FabA and FabZ such that FabZ is preorganized to catalyze only dehydration, while FabA is primed for both dehydration and isomerization.

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