scholarly journals Purification and Characterization of Ornithine Transcarbamylase from Pea (Pisum sativum L.)

1991 ◽  
Vol 96 (1) ◽  
pp. 262-268 ◽  
Author(s):  
Robert D. Slocum ◽  
David P. Richardson
Keyword(s):  
Pisum Sativum ◽  
Ornithine Transcarbamylase ◽  
Purification And Characterization ◽  
Pisum Sativum L

scholarly journals The purification and characterization of a third storage protein (convicilin) from the seeds of pea (Pisum sativum L.)

1980 ◽  
Vol 191 (2) ◽  
pp. 509-516 ◽  
Author(s):  
R R Croy ◽  
J A Gatehouse ◽  
M Tyler ◽  
D Boulter
Keyword(s):  
Genetic Variation ◽  
Pisum Sativum ◽  
Storage Protein ◽  
Purification And Characterization ◽  
Native Form ◽  
Pisum Sativum L ◽  
A Subunit ◽  
Cnbr Cleavage ◽  
Pea Seeds

A third storage protein, distinct from legumin and vicilin, has been purified from the seeds of pea (Pisum sativum L.). This protein has been named ‘convicilin’ and is present in protein bodies isolated from pea seeds. Convicilin has a subunit mol.wt. of 71 000 and a mol.wt. in its native form of 290 000. Convicilin is antigenically dissimilar to legumin, but gives a reaction of identity with vicilin when tested against antibodies raised against both proteins. However, convicilin contains no vicilin subunits and may be clearly separated from vicilin by non-dissociating techniques. Unlike vicilin, convicilin does not interact with concanavalin A, and contains insignificant amounts of carbohydrates. Limited heterogeneity, as shown by isoelectric focusing, N-terminal analysis, and CNBr cleavage, is present in convicilin isolated from a single pea variety; genetic variation of the protein between pea lines has also been observed.

scholarly journals Cloning and characterization of 66 kDa streptavidin-binding peptides (SBP) of Pisum sativum L. embryo specific to var. Alaska

BIOCELL ◽  
2019 ◽  
Vol 43 (3) ◽  
pp. 155-166
Author(s):  
Mahmoud MOUSTAFA ◽  
Saad ALAMRI ◽  
Tarek TAHA ◽  
Ali SHATI ◽  
Sulaiman ALRUMMAN ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Pisum Sativum L ◽  
Binding Peptides ◽  
Streptavidin Binding

Purification and Characterization of a Lectin from Green Split Peas (Pisum sativum)

2015 ◽  
Vol 177 (6) ◽  
pp. 1374-1385 ◽  
Author(s):  
Tzi Bun Ng ◽  
Yau Sang Chan ◽  
Charlene Cheuk Wing Ng ◽  
Jack Ho Wong
Keyword(s):  
Pisum Sativum ◽  
Purification And Characterization

scholarly journals Physiological and Biochemical Characterization of the GABA Shunt Pathway in Pea (Pisum sativum L.) Seedlings Under Drought Stress

Horticulturae ◽  
2021 ◽  
Vol 7 (6) ◽  
pp. 125
Author(s):  
Nisreen A. AL-Quraan ◽  
Zakaria I. Al-Ajlouni ◽  
Nima F. Qawasma
Keyword(s):  
Drought Stress ◽  
Pisum Sativum ◽  
Water Availability ◽  
Dry Weight ◽  
Gaba Shunt ◽  
Pisum Sativum L ◽  
Pea Seedlings ◽  
Mda Content ◽  
Carbohydrate Levels

The physiological and biochemical role of the γ-aminobutyric acid (GABA) shunt pathway in green pea seedlings (Pisum sativum L.) was studied in response to soil water holding capacity levels: 80%, 60%, 40%, 20%, and 10% grown under continuous light at 25 °C for 7 days and 14 days, separately. Characterization of seeds germination pattern, seedlings growth (plant height, fresh and dry weight, and chlorophyll contents), GABA shunt metabolite (GABA, glutamate, and alanine) levels, total protein and carbohydrate levels, and oxidative damage (MDA level) were examined. Data showed a significant effect of drought stress on seed germination, plant growth, GABA shunt metabolites level, total protein and carbohydrate contents, and MDA level. A significant decline in seed germination percentage was recorded at a 20% drought level, which indicated that 20% of soil water holding capacity is the threshold value of water availability for normal germination after 14 days. Seedling fresh weight, dry weight, and plant height were significantly reduced with a positive correlation as water availability was decreased. There was a significant decrease with a positive correlation in Chl a and Chl b contents in response to 7 days and 14 days of drought. GABA shunt metabolites were significantly increased with a negative correlation as water availability decreased. Pea seedlings showed a significant increase in protein content as drought stress was increased. Total carbohydrate levels increased significantly when the amount of water availability decreased. MDA content increased slightly but significantly after 7 days and sharply after 14 days under all water stress levels. The maximum increase in MDA content was observed at 20% and 10% water levels. Overall, the significant increases in GABA, protein and carbohydrate contents were to cope with the physiological impact of drought stress on Pisum sativum L. seedlings by maintaining cellular osmotic adjustment, protecting plants from oxidative stress, balancing carbon and nitrogen (C:N) metabolism, and maintaining cell metabolic homeostasis and cell turgor. The results presented in this study indicated that severe (less than 40% water content of the holding capacity) and long-term drought stress should be avoided during the germination stage to ensure proper seedling growth and metabolism in Pisum sativum L.

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