scholarly journals Stress-Induced Accumulation of Wheat Germ Agglutinin and Abscisic Acid in Roots of Wheat Seedlings

1989 ◽  
Vol 91 (4) ◽  
pp. 1432-1435 ◽  
Author(s):  
Bruno P. A. Cammue ◽  
Willem F. Broekaert ◽  
Jan T. C. Kellens ◽  
Natasha V. Raikhel ◽  
Willy J. Peumans
Keyword(s):  
Abscisic Acid ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Wheat Seedlings

Drought-induced increase in wheat germ agglutinin (WGA) accumulation in developing wheat embryos appears to be independent of ABA

1999 ◽  
Vol 26 (8) ◽  
pp. 787 ◽  
Author(s):  
Priya Bhaglal ◽  
Prabhjeet Singh ◽  
S. S. Bhullar
Keyword(s):  
Triticum Aestivum ◽  
Abscisic Acid ◽  
Water Stress ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Triticum Aestivum L ◽  
Stress Conditions ◽  
Grain Development ◽  
Natural Conditions ◽  
Wheat Embryos

Accumulation of wheat germ agglutinin (WGA) in the developing embryos of three different wheat (Triticum aestivum L.) cultivars, PBW-138, PBW-299 and C-306, was studied in relation to abscisic acid (ABA) accumulation under water stress conditions at 18, 24 and 30 days post anthesis (DPA) under natural conditions. Imposition of water stress in all three cultivars resulted in enhanced ABA levels in the embryos at all stages of grain development. On the contrary, the increase in WGA accumulation in the embryos in response to drought was stage- and cultivar-dependent. Our results suggest that apart from ABA, other factors that are temporally expressed may be involved in drought-induced regulation of the WGA gene.

Wheat germ agglutinin is involved in the protective action of 24-epibrassinolide on the roots of wheat seedlings under drought conditions

2020 ◽  
Vol 146 ◽  
pp. 420-427 ◽  
Author(s):  
Azamat Avalbaev ◽  
Marina Bezrukova ◽  
Chulpan Allagulova ◽  
Alsu Lubyanova ◽  
Guzel Kudoyarova ◽  
...  
Keyword(s):  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Protective Action ◽  
Wheat Seedlings ◽  
Drought Conditions

Failure of 6D1 or Exposure of Platelets to ADP to Affect Agglutination Induced by Wheat Germ Agglutinin

1989 ◽  
Vol 62 (02) ◽  
pp. 815 ◽  
Author(s):  
Marjorie B Zucker ◽  
Robert A Grant ◽  
Evelyn A Mauss
Keyword(s):  
Wheat Germ Agglutinin ◽  
Wheat Germ

Effect of 24-epibrassinolide on localization of ABA, AZP, and dehydrins in wheat plant roots during dehydration

Biomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 329-336
Author(s):  
A.R. Lubyanova ◽  
F.M. Shakirova ◽  
M.V. Bezrukova
Keyword(s):  
Triticum Aestivum ◽  
Wheat Germ ◽  
Wheat Plant ◽  
Triticum Aestivum L ◽  
Immunohistochemical Localization ◽  
Plant Roots ◽  
Wheat Roots ◽  
Wheat Seedlings ◽  
Apoplastic Barrier ◽  
Protective Proteins

We studied the immunohistochemical localization of abscisic acid (ABA), wheat germ agglutinin (WGA) and dehydrins in the roots of wheat seedlings (Triticum aestivum L.) during 24-epibrassinolide-pretreatment (EB-pretreatment) and PEG-induced dehydration. It was found coimmunolocalization of ABA, WGA and dehydrins in the cells of central cylinder of basal part untreated and EB-pretreated roots of wheat seedlings under normal conditions and under osmotic stress. Such mutual localization ABA and protective proteins, WGA and dehydrins, indicates the possible effect of their distribution in the tissues of EB-pretreated wheat roots during dehydration on the apoplastic barrier functioning, which apparently contributes to decrease the water loss under dehydration. Perhaps, the significant localization of ABA and wheat lectin in the metaxylem region enhances EB-induced transport of ABA and WGA from roots to shoots under stress. It can be assumed that brassinosteroids can serve as intermediates in the realization of the protective effect of WGA and wheat dehydrins during water deficit.

Transport Characteristics of Wheat Germ Agglutinin-Modified Insulin-Liposomes and Solid Lipid Nanoparticles in a Perfused Rat Intestinal Model

2006 ◽  
Vol 6 (9) ◽  
pp. 2959-2966 ◽  
Author(s):  
Na Zhang ◽  
Qineng Ping ◽  
Guihua Huang ◽  
Xiuzhen Han ◽  
Yanna Cheng ◽  
...  
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Serum Insulin ◽  
Lipid Nanoparticles ◽  
Solid Lipid ◽  
Mucosal Absorption ◽  
Perfusion Experiment ◽  
Absorption Sites

Wheat germ agglutinin (WGA) modified liposomes and solid lipid nanoparticles (SLNs) were evaluated for improving intestinal absorption of insulin. In an in situ local intestinal perfusion experiment, formulations containing 100 IU/kg insulin were administered to the duodenum, jejunum, and ileum of fasted rats. As hypothesized, ileum was the best intestinal location for the absorption of insulin-containing liposomes. Serum insulin concentrations decreased for the various formulations in different absorption sites according to the following trends: Duodenum > ileum > jejunum for WGA-modified insulin-containing liposomes; duodenum > jejunum > ileum for WGA-modified insulin-containing SLNs; ileum > jejunum > duodenum for insulin-containing liposomes; ileum > duodenum > jejunum for insulin-containing SLNs; and duodenum ≥ ileum > jejunum for aqueous solution of insulin. These results imply that the nanoparticle type and delivery site were important factors with respect to increasing the bioavailability of insulin following oral administration. The proteolytic degradation as well as the epithelial permeability were primary determinants influcing insulin mucosal absorption.

Identification of distinct haemocyte populations from the freshwater bivalves swan mussel (Anodonta cygnea) and duck mussel (Anodonta anatina) using wheat-germ agglutinin

2017 ◽  
Vol 95 (12) ◽  
pp. 937-947 ◽  
Author(s):  
M. Hinzmann ◽  
M. Lopes-Lima ◽  
F. Cerca ◽  
A. Correia ◽  
J. Machado ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Transmission Microscopy ◽  
Anodonta Cygnea ◽  
Functional Studies ◽  
Lectin Staining ◽  
Surface Staining ◽  
Cytological Features ◽  
Freshwater Bivalves

Haemocytes play a major role in molluscs immunity. Functional studies are, however, impaired by limited available experimental tools to identify and sort distinct haemocyte populations. Therefore, using nonlethal methods, we aimed at evaluating whether lectin staining combined with flow cytometry could be used to distinguish circulating haemocyte populations from two freshwater bivalves of the family Unionidae, the duck mussel (Anodonta anatina (L., 1758)) and the swan mussel (Anodonta cygnea (L., 1758)). Based on classical classification, haemocytes were distinguished as granulocytes and hyalinocytes and cytological features were visualized using transmission microscopy and staining techniques. Size, granularity, viability, and surface staining using lectins as specific probes were analysed by flow cytometry and fluorescence microscopy. The microscopic proportions of granulocytes and hyalinocytes significantly differed, being of 70% and 30% for A. cygnea and of 85% and 15% for A. anatina, respectively. Two haemocyte populations were sorted by flow cytometry based on size and granularity and confirmed as granulocytes and hyalinocytes. Interestingly, two different granulocyte populations could be further discriminated in A. cygnea according to their binding affinity to wheat-germ agglutinin (WGA), whereas granulocytes of A. anatina all stained similarly. Our results show that WGA labelling combined with flow cytometry can be used to better discriminate Anodonta haemocyte populations and obtain purified populations for functional studies.

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