scholarly journals Transformation of Brassica napus and Brassica oleracea Using Agrobacterium tumefaciens and the Expression of the bar and neo Genes in the Transgenic Plants

1989 ◽  
Vol 91 (2) ◽  
pp. 694-701 ◽  
Author(s):  
Marc De Block ◽  
Dirk De Brouwer ◽  
Paul Tenning
Keyword(s):  
Brassica Napus ◽  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Brassica Oleracea

Agrobacterium tumefaciens-mediated Transformation of Double Haploid Canola (Brassica napus) Lines

1997 ◽  
Vol 24 (1) ◽  
pp. 97 ◽  
Author(s):  
K. Kazan ◽  
M. D. Curtis ◽  
K. C. Goulter ◽  
J. M. Manners
Keyword(s):  
Brassica Napus ◽  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Genetic Transformation ◽  
Double Haploid ◽  
Gene Promoter ◽  
Root Explants ◽  
Hypocotyl Explants ◽  
Peroxidase Gene ◽  
Hypocotyl Segments

Double haploid (DH) genotypes of canola (Brassica napus L.) have a high level of genetic uniformity but have not been previously tested for genetic transformation. Transgenic plants from three of four DH genotypes derived from cv. Westar were obtained by inoculation of either hypocotyl segments or root explants with Agrobacterium tumefaciens. For hypocotyl transformation, A. tumefaciens strain LBA4404 containing a binary plasmid with the neomycin phosphotransferase gene (nptII) and a CaMV 35S-peroxidase gene cassette was co-cultivated with hypocotyl segments taken from the 5–6-day-old seedlings. Transformation frequencies for hypocotyl explants of two DH genotypes were 0.3–3%. Direct evidence for genetic transformation of hypocotyl explants was obtained through molecular hybridisation analysis. Using this protocol, mature transformed plants were obtained within 4–6 months of co-cultivation. A method of root transformation was successfully modified for one DH genotype of canola and transgenic plants were obtained at a frequency of 2%. Using this protocol, a peroxidase gene promoter–GUS fusion construct was introduced into a DH genotype. Tissue specific GUS expression driven by the peroxidase gene promoter in transgenic plants was analysed by GUS staining. Transformation systems for double haploid canola lines will permit the assessment of introduced genes for their effect on agronomic and physiological traits.

T-DNA integration patterns in transgenic plants mediated by Agrobacterium tumefaciens

2011 ◽  
Vol 33 (12) ◽  
pp. 1327-1334 ◽  
Author(s):  
Lin YANG ◽  
Feng-Ling FU ◽  
Wan-Chen LI
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Dna Integration

Resynthesis of Brassica napus with Brassica oleracea or Brassica rapa Cytoplasm

2010 ◽  
Vol 36 (8) ◽  
pp. 1280-1285 ◽  
Author(s):  
Jun LI ◽  
Li-Xia LUO ◽  
Zhuan WANG ◽  
Jun LI ◽  
Kun-Rong CHEN ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Brassica Oleracea ◽  
Brassica Rapa

scholarly journals Dehiscence-related expression of an Arabidopsis thaliana gene encoding a polygalacturonase in transgenic plants of Brassica napus

1999 ◽  
Vol 22 (2) ◽  
pp. 159-167 ◽  
Author(s):  
E. S. JENKINS ◽  
W. PAUL ◽  
M. CRAZE ◽  
C. A. WHITELAW ◽  
A. WEIGAND ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Brassica Napus ◽  
Transgenic Plants ◽  
Gene Encoding

scholarly journals A Simple Procedure for Mesophyll Protoplast Culture and Plant Regeneration in Brassica oleracea L. and Brassica napus L.

2011 ◽  
Vol 42 (No. 3) ◽  
pp. 103-110 ◽  
Author(s):  
N.D. Kaur ◽  
M. Vyvadilová ◽  
M. Klíma ◽  
M. Bechyně
Keyword(s):  
Brassica Napus ◽  
Plant Regeneration ◽  
Brassica Oleracea ◽  
Growth Regulators ◽  
Protoplast Culture ◽  
Treatment Time ◽  
Simple Procedure ◽  
Enzyme Treatment ◽  
Induction Medium ◽  
Brassica Napus L

An improved protocol for Brassica protoplast culture and plant regeneration was developed. Isolated protoplasts from four-weeks-old in vitro shoot tip culture of Brassica oleracea var. botrytis cv. Siria F1 and Brassica napus doubled haploid of breeding line OP-1 were cultured at a density of 9.8&ndash;11.2 &times; 10<sup>4 </sup>protoplasts/ml in darkness at 25&deg;C in a modified medium containing 2% glucose, 0.25 mg/l 2,4-D, 1 mg/l BAP and 1 mg/l NAA. The first divisions of protoplasts were observed on the third day of culture in B. oleracea and on the fourth day in B. napus. The protoplast cultures were diluted with low osmotic medium on 7<sup>th</sup> and 11<sup>th</sup> day. The frequency of dividing cells was about 80% in B. oleracea and 50% in B. napus. After one month, the microcalli of approximately 0.5&ndash;1 mm in size were transferred into an induction medium with various combinations of growth regulators. Minimum duration of enzyme treatment time and extended dark period in the initial phase of culture increased the survival rate of protoplasts. Organogenesis started when the calli enlarged in size on an induction medium (1 mg/l NAA, 0.02 mg/l GA<sub>3</sub>, 1 mg/l 2iP) with 2% sucrose and 0.8% agar. Regeneration frequency of calli was found to be 69&ndash;75% in B. oleracea and 2&ndash;3% in B. napus. Well-developed shoots were transferred for rooting to a half-strength MS medium without growth regulators. More than 100 B. oleracea regenerants were transferred into soil, and they produced normal heads and set seeds. This very simple procedure is efficient and suitable mainly for B. oleracea var. botrytis and represents a background for fusion experiments. &nbsp;

Do low-ethylene-producing transgenic canola (Brassica napus) plants expressing the ACC deaminase gene differ from wild-type plants in response to UVB radiation?

2007 ◽  
Vol 85 (2) ◽  
pp. 148-159 ◽  
Author(s):  
Mirwais M. Qaderi ◽  
M. Anisul Islam ◽  
David M. Reid ◽  
Saleh Shah
Keyword(s):  
Brassica Napus ◽  
Transgenic Plants ◽  
Photosynthetic Pigment ◽  
Physiological Parameters ◽  
Ultraviolet B ◽  
Differential Sensitivity ◽  
Plant Responses ◽  
Uvb Radiation ◽  
Wild Type ◽  
Ethylene Evolution

Few studies have considered ethylene involvement in plant responses to ultraviolet-B (UVB) radiation. We studied the responses to UVB radiation of one wild-type (WT, ‘Westar’) canola (Brassica napus L.) with normal ethylene evolution and two transgenic (C1, C2) lines with lower ethylene evolution. Canola plants were grown under biologically effective levels of UVB (UVBBE) radiation: 0.03 (low), 4.88 (medium), and 9.78 (high) kJ·m–2·d–1 in controlled-environment growth chambers. The growth and physiological parameters of the plants were measured. Of the two transgenic lines, C1 demonstrated higher ethylene evolution than C2 but lower than WT. The lowest aboveground and belowground biomass was found with exposure to high UVB radiation. WT produced more biomass than C2. Net CO2 assimilation and transpiration did not vary among plant lines or UVB treatments. Water-use efficiency was lower under high UVB radiation than under low UVB. The quantum yield of photosystem II was higher for C2 than for either WT or C1. WT did not differ from transgenic plants in respect to photosynthetic pigments and UV-screening compounds. Photosynthetic pigment concentration decreased, but concentration of UV-screening compounds, thickness of epicuticular wax, and the rate of root hydraulic conductance were increased by exposure to UVB radiation. While there appears to be a lack of ethylene involvement in some of the measured physiological parameters, the transgenic plants exhibited differential sensitivity to UVB in a few key measured parameters.

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