scholarly journals Identification and Characterization of the Ca2+-ATPase which Drives Active Transport of Ca2+ at the Plasma Membrane of Radish Seedlings

1989 ◽  
Vol 90 (4) ◽  
pp. 1429-1434 ◽  
Author(s):  
Franca Rasi-Caldogno ◽  
Maria Chiara Pugliarello ◽  
Claudio Olivari ◽  
Maria Ida De Michelis
Keyword(s):  
Plasma Membrane ◽  
Active Transport ◽  
Radish Seedlings ◽  
Identification And Characterization

scholarly journals Identification and Characterization of SNQ2, a New Multidrug ATP Binding Cassette Transporter of the Yeast Plasma Membrane

1995 ◽  
Vol 270 (30) ◽  
pp. 18150-18157 ◽  
Author(s):  
Anabelle Decottignies ◽  
Laurence Lambert ◽  
Patrice Catty ◽  
Herv Degand ◽  
Eric A. Epping ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Atp Binding ◽  
Yeast Plasma Membrane ◽  
Atp Binding Cassette Transporter ◽  
Identification And Characterization ◽  
Atp Binding Cassette

scholarly journals Identification and characterization of Cardiac Glycosides as senolytic compounds

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Francisco Triana-Martínez ◽  
Pilar Picallos-Rabina ◽  
Sabela Da Silva-Álvarez ◽  
Federico Pietrocola ◽  
Susana Llanos ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Lung Fibrosis ◽  
Cardiac Glycosides ◽  
Experimental Approach ◽  
Therapeutic Interventions ◽  
Electrochemical Gradient ◽  
Age Related ◽  
Identification And Characterization

Abstract Compounds with specific cytotoxic activity in senescent cells, or senolytics, support the causal involvement of senescence in aging and offer therapeutic interventions. Here we report the identification of Cardiac Glycosides (CGs) as a family of compounds with senolytic activity. CGs, by targeting the Na+/K+ATPase pump, cause a disbalanced electrochemical gradient within the cell causing depolarization and acidification. Senescent cells present a slightly depolarized plasma membrane and higher concentrations of H+, making them more susceptible to the action of CGs. These vulnerabilities can be exploited for therapeutic purposes as evidenced by the in vivo eradication of tumors xenografted in mice after treatment with the combination of a senogenic and a senolytic drug. The senolytic effect of CGs is also effective in the elimination of senescence-induced lung fibrosis. This experimental approach allows the identification of compounds with senolytic activity that could potentially be used to develop effective treatments against age-related diseases.

scholarly journals Identification and Characterization of Plasma Membrane Proteins that Bind to Microtubules in Pollen Tubes and Generative Cells of Tobacco

2005 ◽  
Vol 46 (4) ◽  
pp. 563-578 ◽  
Author(s):  
Giampiero Cai ◽  
Elisa Ovidi ◽  
Silvia Romagnoli ◽  
Marylin Vantard ◽  
Mauro Cresti ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Pollen Tubes ◽  
Plasma Membrane Proteins ◽  
Identification And Characterization ◽  
Generative Cells

Identification and Characterization of a Family of Genes for the Plasma Membrane H+-ATPase of Oryza sativa L.

1994 ◽  
Vol 35 (8) ◽  
pp. 1251-1256 ◽  
Author(s):  
Tetsuya Ookura ◽  
Masato Wada ◽  
Yoshikiyo Sakakibara ◽  
Kim Hyun Jeong ◽  
Issei Maruta ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Plasma Membrane ◽  
Oryza Sativa L ◽  
Identification And Characterization

scholarly journals Identification and characterization of the 2D6 and Mr 23000 antigens on the plasma membrane of rat spermatozoa

1987 ◽  
Vol 241 (2) ◽  
pp. 353-360 ◽  
Author(s):  
R Jones ◽  
C R Brown
Keyword(s):  
Plasma Membrane ◽  
Antigenic Determinant ◽  
Egg Recognition ◽  
Western Blots ◽  
One Dimensional ◽  
Antigen Present ◽  
Membrane Bound ◽  
Species Specific ◽  
Identification And Characterization

Previous investigations [Jones, Brown, von Glos & Gaunt (1985) Exp. Cell Res. 156, 31-44] have demonstrated the appearance of a new antigenic determinant (recognized by monoclonal antibody 2D6) on the plasma membrane of rat spermatozoa during post-testicular maturation in the epididymis. Identification of the 2D6 antigen on Western blots from one-dimensional SDS/polyacrylamide gels revealed that it co-migrated with a membrane protein (designated Mr 23,000 antigen) present on testicular and immature germ cells, suggesting that one antigen might be a modified version of the other. In the present work, however, we demonstrate that, although they have similar Mr and are present in soluble and membrane-bound forms, the 2D6 and Mr 23,000 antigens are biochemically and immunologically distinct molecules. The properties of the antigens are described and compared. The Mr 23,000 antigen is present on both testicular and cauda epididymidal spermatozoa, has a pI of 6.1, contains no detectable carbohydrate, is not tissue-specific and is degraded by V8 protease. By contrast, the 2D6 antigen is glycosylated, has a broad pI from 4.5 to 6.1, is tissue- and species-specific and is resistant to digestion with V8 protease. Its role in sperm-egg recognition is discussed.

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