scholarly journals Cell Wall Proteins from Sugar Beet Cells in Suspension Culture

1989 ◽  
Vol 89 (2) ◽  
pp. 712-716 ◽  
Author(s):  
Hiroshi Masuda ◽  
Seiichi Komiyama ◽  
Shiro Sugawara
Keyword(s):  
Cell Wall ◽  
Sugar Beet ◽  
Suspension Culture ◽  
Cell Wall Proteins

scholarly journals Draft Genome Sequence of the Bacterium Gordonia jacobaea , a New Member of the Gordonia Genus

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
G. Jiménez-Galisteo ◽  
T. G. Villa ◽  
T. Vinuesa ◽  
M. Viñas ◽  
A. Domínguez ◽  
...  
Keyword(s):  
Cell Wall ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Cell Wall Proteins ◽  
Santiago De Compostela

Gordonia jacobaea was isolated and characterized in the Department of Microbiology, University of Santiago de Compostela, in 2000. Here we present the draft genome sequence of this species, which will improve our understanding of the diversity and the relation of the cell wall proteins of G. jacobaea with other mycolata.

scholarly journals Purification and Properties of Glucoamylase from Sugar Beet Cells in Suspension Culture

1988 ◽  
Vol 88 (1) ◽  
pp. 172-177 ◽  
Author(s):  
Hiroshi Masuda ◽  
Manabu Murata ◽  
Toshimasa Takahashi ◽  
Shiro Sugawara
Keyword(s):  
Sugar Beet ◽  
Suspension Culture ◽  
Purification And Properties

scholarly journals Proteomic Analysis ofPhytophthora infestansReveals the Importance of Cell Wall Proteins in Pathogenicity

2017 ◽  
Vol 16 (11) ◽  
pp. 1958-1971 ◽  
Author(s):  
Svante Resjö ◽  
Maja Brus ◽  
Ashfaq Ali ◽  
Harold J. G. Meijer ◽  
Marianne Sandin ◽  
...  
Keyword(s):  
Cell Wall ◽  
Proteomic Analysis ◽  
Cell Wall Proteins

scholarly journals A proposed nomenclature for cell wall proteins of Clostridium difficile

2011 ◽  
Vol 60 (8) ◽  
pp. 1225-1228 ◽  
Author(s):  
Robert P. Fagan ◽  
Claire Janoir ◽  
Anne Collignon ◽  
Paola Mastrantonio ◽  
Ian R. Poxton ◽  
...  
Keyword(s):  
Cell Wall ◽  
Clostridium Difficile ◽  
Cell Wall Proteins

scholarly journals Homologous Recombination in Clostridioides difficile Mediates Diversification of Cell Surface Features and Transport Systems

mSphere ◽  
2020 ◽  
Vol 5 (6) ◽  
Author(s):  
Hannah D. Steinberg ◽  
Evan S. Snitkin
Keyword(s):  
Cell Wall ◽  
Homologous Recombination ◽  
Sugar Transport ◽  
The United States ◽  
Strain Typing ◽  
Cell Wall Proteins ◽  
Genetic Loci ◽  
Content Type ◽  
Clostridioides Difficile ◽  
Host Pathogen

ABSTRACT Illness caused by the pathogen Clostridioides difficile is widespread and can range in severity from mild diarrhea to sepsis and death. Strains of C. difficile isolated from human infections exhibit great genetic diversity, leading to the hypothesis that the genetic background of the infecting strain at least partially determines a patient’s clinical course. However, although certain strains of C. difficile have been suggested to be associated with increased severity, strain typing alone has proved insufficient to explain infection severity. The limited explanatory power of strain typing has been hypothesized to be due to genetic variation within strain types, as well as genetic elements shared between strain types. Homologous recombination is an evolutionary mechanism that can result in large genetic differences between two otherwise clonal isolates, and also lead to convergent genotypes in distantly related strains. More than 400 C. difficile genomes were analyzed here to assess the effect of homologous recombination within and between C. difficile clades. Almost three-quarters of single nucleotide variants in the C. difficile phylogeny are predicted to be due to homologous recombination events. Furthermore, recombination events were enriched in genes previously reported to be important to virulence and host-pathogen interactions, such as flagella, cell wall proteins, and sugar transport and metabolism. Thus, by exploring the landscape of homologous recombination in C. difficile, we identified genetic loci whose elevated rates of recombination mediated diversification, making them strong candidates for being mediators of host-pathogen interaction in diverse strains of C. difficile. IMPORTANCE Infections with C. difficile result in up to half a million illnesses and tens of thousands of deaths annually in the United States. The severity of C. difficile illness is dependent on both host and bacterial factors. Studying the evolutionary history of C. difficile pathogens is important for understanding the variation in pathogenicity of these bacteria. This study examines the extent and targets of homologous recombination, a mechanism by which distant strains of bacteria can share genetic material, in hundreds of C. difficile strains and identifies hot spots of realized recombination events. The results of this analysis reveal the importance of homologous recombination in the diversification of genetic loci in C. difficile that are significant in its pathogenicity and host interactions, such as flagellar construction, cell wall proteins, and sugar transport and metabolism.

scholarly journals Slipped strand mispairing in the gene encoding sialidase NanH3 in Gardnerella spp

2020 ◽  
Author(s):  
Shakya P. Kurukulasuriya ◽  
Mo H. Patterson ◽  
Janet E. Hill
Keyword(s):  
Cell Wall ◽  
Biofilm Formation ◽  
Gene Sequence ◽  
Phase Variation ◽  
Cell Wall Proteins ◽  
Vaginal Microbiome ◽  
Gene Encoding ◽  
Reading Frame ◽  
Sialidase Activity ◽  
Mucosal Surfaces

AbstractCell wall proteins with sialidase activity are involved in carbohydrate assimilation, adhesion to mucosal surfaces, and biofilm formation. Gardnerella spp. inhabit the human vaginal microbiome and encode up to three sialidase enzymes, two of which are suspected to be cell wall associated. Here we demonstrate that the gene encoding extracellular sialidase NanH3 is found almost exclusively in G. piotii and closely related Gardnerella genome sp. 3, and its presence correlates with sialidase positive phenotype in a collection of 112 Gardnerella isolates. The nanH3 gene sequence includes a homopolymeric repeat of cytosines that varies in length within cell populations, indicating that this gene is subject to slipped-strand mispairing, a mechanisms of phase variation in bacteria. Variation in the length of the homopolymer sequence results in encoding of either the full length sialidase protein or truncated peptides lacking the sialidase domain due to introduction of reading-frame shifts and premature stop codons. Phase variation in NanH3 may be involved in immune evasion or modulation of adhesion to host epithelial cells, and formation of biofilms characteristic of the vaginal dysbiosis known as bacterial vaginosis.

scholarly journals Defining Staphylococcus epidermidis cell wall proteins.

1990 ◽  
Vol 28 (12) ◽  
pp. 2757-2760 ◽  
Author(s):  
C C Patrick ◽  
M R Plaunt ◽  
S M Sweet ◽  
G S Patrick
Keyword(s):  
Cell Wall ◽  
Staphylococcus Epidermidis ◽  
Cell Wall Proteins
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