scholarly journals Kinetics of Determination in the Differentiation of Isolated Mesophyll Cells of Zinnia elegans to Tracheary Elements

1988 ◽  
Vol 88 (1) ◽  
pp. 92-96 ◽  
Author(s):  
Diane L. Church ◽  
Arthur W. Galston
Keyword(s):  
Zinnia Elegans ◽  
Tracheary Elements ◽  
Mesophyll Cells ◽  
Kinetics Of ◽  
Isolated Mesophyll Cells

Comparison between tracheary element lignin formation and extracellular lignin-like substance formation during the culture of isolated Zinnia elegans mesophyll cells

Biologia ◽  
2011 ◽  
Vol 66 (1) ◽  
Author(s):  
Yasushi Sato ◽  
Youko Yajima ◽  
Naohito Tokunaga ◽  
Ross Whetten
Keyword(s):  
Peroxidase Activity ◽  
Cell Walls ◽  
Vascular Plants ◽  
Culture Media ◽  
Tracheary Element ◽  
Zinnia Elegans ◽  
Tracheary Elements ◽  
Mesophyll Cells ◽  
Isolated Mesophyll Cells ◽  
Lignin Deposition

AbstractLignin is synthesized not only during morphogenesis of vascular plants but also in response to various stresses. Isolated Zinnia elegans mesophyll cells can differentiate into tracheary elements (TEs), and deposit lignin into cell walls in TE-inductive medium (D medium). Meanwhile isolated mesophyll cells cultured in hormone-free medium (Co medium) accumulate stress lignin-like substance during culture. Therefore this culture system is suitable for study of lignin and lignin-like substance formation.In D medium lignin was deposited in TEs, but in Co medium, extracellular lignin-like substance accumulated. Analysis of the culture media indicated the presence of dilignols in D culture, but not in Co culture. To investigate the fate of lignin precursors, we added coniferyl alcohol (CA) in each culture. In Co medium, CA was polymerized into dilignols rapidly but they were present only temporarily, and in D medium CA was polymerized into dilignols relatively slowly but their content increased continually.Meanwhile, in Co culture, peroxidase activity in the medium was much higher than the peroxidase activity bound ionically to the cell walls. In D culture, ionically bound peroxidase activity was higher than that in the medium. These results may suggest that lignin deposition in TEs is related to ionically bound peroxidases in D culture, and lignin-like substance deposition in the medium is related to peroxidases in the medium in Co culture.

Activity of cell-wall degradation associated with differentiation of isolated mesophyll cells of Zinnia elegans into tracheary elements

Planta ◽  
2002 ◽  
Vol 215 (2) ◽  
pp. 177-184 ◽  
Author(s):  
Youichi Ohdaira ◽  
Koichi Kakegawa ◽  
Shin-ichi Amino ◽  
Munetaka Sugiyama ◽  
Hiroo Fukuda
Keyword(s):  
Cell Wall ◽  
Zinnia Elegans ◽  
Cell Wall Degradation ◽  
Tracheary Elements ◽  
Mesophyll Cells ◽  
Isolated Mesophyll Cells
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