scholarly journals A Second Extensin-Like Hydroxyproline-Rich Glycoprotein from Carrot Cell Walls

1987 ◽  
Vol 84 (3) ◽  
pp. 820-825 ◽  
Author(s):  
Joel P. Stafstrom ◽  
L. Andrew Staehelin
Keyword(s):  
Cell Walls ◽  
Carrot Cell

scholarly journals Cross-Linking Patterns in Salt-Extractable Extensin from Carrot Cell Walls

1986 ◽  
Vol 81 (1) ◽  
pp. 234-241 ◽  
Author(s):  
Joel P. Stafstrom ◽  
L. Andrew Staehelin
Keyword(s):  
Cell Walls ◽  
Cross Linking ◽  
Carrot Cell

Characterization and Distribution of Phenolics in Carrot Cell Walls

2008 ◽  
Vol 56 (18) ◽  
pp. 8558-8564 ◽  
Author(s):  
Yoon-Han Kang ◽  
Charlotte C. Parker ◽  
Andrew C. Smith ◽  
Keith W. Waldron
Keyword(s):  
Cell Walls ◽  
Carrot Cell

FUSION OF CARROT AND BARLEY PROTOPLASTS AND DIVISION OF HETEROKARYOCYTES

1976 ◽  
Vol 18 (2) ◽  
pp. 263-269 ◽  
Author(s):  
D. Dudits ◽  
K. N. Kao ◽  
F. Constabel ◽  
O. L. Gamborg
Keyword(s):  
Cell Walls ◽  
Culture Medium ◽  
Cultured Cells ◽  
Differential Staining ◽  
Hordeum Vulgare L ◽  
Daucus Carota L ◽  
Carrot Cell ◽  
Fusion Of Protoplasts ◽  
Fusion Products ◽  
Carrot Cell Cultures

Fusion of protoplasts from cultured cells of carrot (Daucus carota L.) and from leaves of barley (Hordeum vulgare L.) by means of polyethylene glycol resulted in the formation of 4-5 fusion products (heterokaryocytes) per 100 protoplasts. When incubated in culture medium, the heterokaryocytes regenerated cell walls and divided. The frequency of division depended on the viability of the protoplasts from carrot cell cultures, specifically, on the mitotic activity of the cells. Fusion of interphase carrot and barley nuclei was detected by differential staining. Synchronized mitosis was observed in heterokaryons containing barley and carrot nuclei.

Serine-O-galactosyl linkages in glycopeptides from carrot cell walls

1976 ◽  
Vol 15 (1) ◽  
pp. 165-169 ◽  
Author(s):  
Cho Yong-Pill ◽  
Maarten J. Chrispeels
Keyword(s):  
Cell Walls ◽  
Carrot Cell

Ester-Linked Phenolic Components of Carrot Cell Walls

1997 ◽  
Vol 45 (7) ◽  
pp. 2468-2471 ◽  
Author(s):  
Adrian J. Parr ◽  
Annie Ng ◽  
Keith W. Waldron
Keyword(s):  
Cell Walls ◽  
Phenolic Components ◽  
Carrot Cell

The self-assembled network and physiological degradation of pectins in carrot cell walls

2015 ◽  
Vol 43 ◽  
pp. 41-50 ◽  
Author(s):  
Justyna Cybulska ◽  
Artur Zdunek ◽  
Arkadiusz Kozioł
Keyword(s):  
Cell Walls ◽  
The Self ◽  
Carrot Cell ◽  
Self Assembled

Effect of .gamma. radiation of polysaccharides and calcium distribution in carrot cell walls

1970 ◽  
Vol 18 (5) ◽  
pp. 878-880 ◽  
Author(s):  
Ronald J. Echandi ◽  
Betty R. Chase ◽  
Louis M. Massey
Keyword(s):  
Gamma Radiation ◽  
Cell Walls ◽  
Calcium Distribution ◽  
Carrot Cell

scholarly journals Effect of Storage on Rheology of Water-Soluble, Chelate-Soluble and Diluted Alkali-Soluble Pectin in Carrot Cell Walls

2014 ◽  
Vol 8 (1) ◽  
pp. 171-180 ◽  
Author(s):  
Joanna Mierczyńska ◽  
Justyna Cybulska ◽  
Piotr M. Pieczywek ◽  
Artur Zdunek
Keyword(s):  
Cell Walls ◽  
Water Soluble ◽  
Carrot Cell

scholarly journals Identification and characterization of plant glycerophosphodiester phosphodiesterase

2004 ◽  
Vol 379 (3) ◽  
pp. 601-607 ◽  
Author(s):  
Benoît van der REST ◽  
Norbert ROLLAND ◽  
Anne-Marie BOISSON ◽  
Myriam FERRO ◽  
Richard BLIGNY ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Cell Walls ◽  
Expression System ◽  
Gel Filtration ◽  
Cell Suspension Cultures ◽  
Cell Wall Proteins ◽  
Ammonium Sulphate Precipitation ◽  
Carrot Cell ◽  
Glycerophosphodiester Phosphodiesterase

GPX-PDE (glycerophosphodiester phosphodiesterase; EC 3.1.4.46) is a relatively poorly characterized enzyme that catalyses the hydrolysis of various glycerophosphodiesters (glycerophosphocholine, glycerophosphoethanolamine, glycerophosphoglycerol, glycerophosphoserine and bis-glycerophosphoglycerol), releasing sn-glycerol 3-phosphate and the corresponding alcohol. In a previous study, we demonstrated the existence of a novel GPX-PDE in the cell walls and vacuoles of plant cells. Since no GPX-PDE had been identified in any plant organism, the purification of GPX-PDE from carrot cell walls was attempted. After extraction of cell wall proteins from carrot cell suspension cultures with CaCl2, GPX-PDE was purified up to 2700-fold using, successively, ammonium sulphate precipitation, gel filtration and concanavalin A–Sepharose. Internal sequence analysis of a 55 kDa protein identified in the extract following 2700-fold purification revealed strong similarity to the primary sequence of GLPQ, a bacterial GPX-PDE. To confirm the identity of plant GPX-PDE, an Arabidopsis thaliana cDNA similar to that encoding the bacterial GPX-PDE was cloned and overexpressed in a bacterial expression system, and was used to raise antibodies against the putative Arabidopsis thaliana GPX-PDE. Immunochemical assays performed on carrot cell wall proteins extracted by CaCl2 treatment showed a strong correlation between GPX-PDE activity and detection of the 55 kDa protein, validating the identity of the plant GPX-PDE. Finally, various properties of the purified enzyme were investigated. GPX-PDE is a multimeric enzyme, specific for glycerophosphodiesters, exhibiting a Km of 36 µM for glycerophosphocholine and active within a wide pH range (from 4 to 10). Since these properties are similar to those of GLPQ, the bacterial GPX-PDE, the similarities between plant and bacterial enzymes are also discussed.

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