scholarly journals The Effect of Extracellular Components from Colletotrichum lindemuthianum on Membrane Transport in Vesicles Isolated from Bean Hypocotyl

1987 ◽  
Vol 84 (2) ◽  
pp. 428-432 ◽  
Author(s):  
Kim R. Rogers ◽  
Anne J. Anderson
Keyword(s):  
Membrane Transport ◽  
Colletotrichum Lindemuthianum ◽  
Bean Hypocotyl

Response of Phaseolus vulgaris protoplasts to chemical components of fungal origin

1987 ◽  
Vol 65 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Helen M. Griffiths ◽  
Anne J. Anderson
Keyword(s):  
Phaseolus Vulgaris ◽  
French Bean ◽  
Colletotrichum Lindemuthianum ◽  
Chemical Components ◽  
Low Concentrations ◽  
Extracellular Products ◽  
Bean Hypocotyl ◽  
Whole Plants ◽  
Hypocotyl Tissue ◽  
Plant Pathogen Interactions

Colletotrichum lindemuthianum is the causal agent of anthracnose in Phaseolus vulgaris L. (French bean). The α and β races of the fungus were used in this study with French bean cultivar ‘Great Northern’. Whole plants inoculated with the α race developed brown lesions on the hypocotyls (susceptible response). The β race caused small limited lesions, indicating a more resistant interaction. Extracellular products and cell wall materials were isolated from β race cultures and extracellular products from α race cultures. The extracts were size fractionated. By using a cotyledon bioassay, elicitor activity was demonstrated on ‘Dark Red Kidney’ within fractions from the α race. Fractions from the β race had little activity on ‘Dark Red Kidney’ or ‘Great Northern’ cotyledons. Protoplasts were isolated from ‘Great Northern’ bean hypocotyl tissue and incubated with the fungal fractions. Even at low concentrations (0.01 μg glucose equivalent∙mL−1), the β race culture filtrate rapidly killed a greater percentage of protoplasts (30%) than the α race (15%). The β race wall extract had little effect upon protoplast viability. The proportion of nonviable protoplasts depended on the incubation period and the concentration of the fungal material. Heat treatment of the culture filtrates and wall extract did not decrease their lethal effects. The results suggest that protoplasts may be valuable in examining the nature of certain plant–pathogen interactions.

Biological Activity of Trans-Membrane Anion Carriers

2018 ◽  
Vol 25 (30) ◽  
pp. 3560-3576 ◽  
Author(s):  
Massimo Tosolini ◽  
Paolo Pengo ◽  
Paolo Tecilla
Keyword(s):  
Biological Activity ◽  
Membrane Transport ◽  
Anticancer Agents ◽  
Biological Effects ◽  
Structure Activity Relationship ◽  
New Drugs ◽  
Activity Relationship ◽  
Anion Channels ◽  
Cellular Homeostasis ◽  
Structure Activity

Natural and synthetic anionophores promote the trans-membrane transport of anions such as chloride and bicarbonate. This process may alter cellular homeostasis with possible effects on internal ions concentration and pH levels triggering several and diverse biological effects. In this article, an overview of the recent results on the study of aniontransporters, mainly acting with a carrier-type mechanism, is given with emphasis on the structure/activity relationship and on their biological activity as antibiotic and anticancer agents and in the development of new drugs for treating conditions derived from dysregulation of natural anion channels.

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