scholarly journals Further Characterization of Ribosome Binding to Thylakoid Membranes

1987 ◽  
Vol 84 (1) ◽  
pp. 31-34 ◽  
Author(s):  
Josh Hurewitz ◽  
André T. Jagendorf
Keyword(s):  
Thylakoid Membranes ◽  
Ribosome Binding

Electron microscope and physical chemical characterization of C-phycocyanin from fresh extracts of two blue-green algae

1973 ◽  
Vol 19 (7) ◽  
pp. 831-836 ◽  
Author(s):  
Martin Kessel ◽  
Robert MacColl ◽  
Donald S. Berns ◽  
Mercedes R. Edwards
Keyword(s):  
Red Light ◽  
Chemical Characterization ◽  
Thylakoid Membranes ◽  
Sedimentation Velocity ◽  
Particulate Material ◽  
Thin Sections ◽  
Blue Green Algae ◽  
Physical Chemical ◽  
Sedimentation Velocity Centrifugation

The particulate material (intact and dissociated phycobilisomes), adjacent to the thylakoids of Plectonema boryanum and Calothrix parietina, was examined in thin sections and in freshly prepared C-phycocyanin extracts. For the latter a method was developed using spheroplasts of log-phase cells. When examined by sedimentation velocity centrifugation, the sedimentation coefficients of the rapidly extracted C-phycocyanin were found to be 18 S and 5 S. Also, a 10-S boundary was observed with P. boryanum. When C. parietina was grown under red light the 18-S aggregates increased to 20 S. The 18 S particles, in electron micrographs of negatively stained preparations, displayed eight subunits surrounding a central one. The possibility that some subunits fall out of the plane of view is contemplated in proposing a dodecamer arrangement for such particles. The 20-S particles were also examined by electron microscopy. However, the precise number of monomer units associated with either the 18-S or 20-S particles has not yet been ascertained. Thin sections of intact algal cells are presented to demonstrate the presence of phycobilisomes along the outer faces of the thylakoid membranes.

Mass spectrometric characterization of photooxidative protein modifications in Arabidopsis thaliana thylakoid membranes

2010 ◽  
Vol 25 (1) ◽  
pp. 184-190 ◽  
Author(s):  
Dmitry Galetskiy ◽  
Jens N. Lohscheider ◽  
Alexey S. Kononikhin ◽  
Igor A. Popov ◽  
Eugene N. Nikolaev ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Thylakoid Membranes ◽  
Mass Spectrometric ◽  
Protein Modifications
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