scholarly journals Characterization and Solubilization of the Alternative Oxidase of Sauromatum guttatum Mitochondria

1986 ◽  
Vol 82 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Thomas E. Elthon ◽  
Lee McIntosh
Keyword(s):  
Alternative Oxidase ◽  
Sauromatum Guttatum

scholarly journals Immunological identification of the alternative oxidase of Neurospora crassa mitochondria.

1989 ◽  
Vol 9 (3) ◽  
pp. 1362-1364 ◽  
Author(s):  
A M Lambowitz ◽  
J R Sabourin ◽  
H Bertrand ◽  
R Nickels ◽  
L McIntosh
Keyword(s):  
Neurospora Crassa ◽  
Oxidase Activity ◽  
Hydroxamic Acid ◽  
Alternative Oxidase ◽  
Fungal Species ◽  
The Other ◽  
Wild Type ◽  
Higher Plant ◽  
Sauromatum Guttatum ◽  
Immunological Identification

Neurospora crassa mitochondria use a branched electron transport system in which one branch is a conventional cytochrome system and the other is an alternative cyanide-resistant, hydroxamic acid-sensitive oxidase that is induced when the cytochrome system is impaired. We used a monoclonal antibody to the alternative oxidase of the higher plant Sauromatum guttatum to identify a similar set of related polypeptides (Mr, 36,500 and 37,000) that was associated with the alternative oxidase activity of N. crassa mitochondria. These polypeptides were not present constitutively in the mitochondria of a wild-type N. crassa strain, but were produced in high amounts under conditions that induced alternative oxidase activity. Under the same conditions, mutants in the aod-1 gene, with one exception, produced apparently inactive alternative oxidase polypeptides, whereas mutants in the aod-2 gene failed to produce these polypeptides. The latter findings support the hypothesis that aod-1 is a structural gene for the alternative oxidase and that the aod-2 gene encodes a component that is required for induction of alternative oxidase activity. Finally, our results indicate that the alternative oxidase is highly conserved, even between plant and fungal species.

scholarly journals Mutagenesis of the Sauromatum guttatum alternative oxidase reveals features important for oxygen binding and catalysis

2010 ◽  
Vol 1797 (6-7) ◽  
pp. 732-737 ◽  
Author(s):  
Paul G. Crichton ◽  
Mary S. Albury ◽  
Charles Affourtit ◽  
Anthony L. Moore
Keyword(s):  
Alternative Oxidase ◽  
Oxygen Binding ◽  
Sauromatum Guttatum

scholarly journals Pyruvate transport by thermogenic-tissue mitochondria

1987 ◽  
Vol 247 (2) ◽  
pp. 441-447 ◽  
Author(s):  
M O Proudlove ◽  
R B Beechey ◽  
A L Moore
Keyword(s):  
Alternative Oxidase ◽  
Competitive Inhibition ◽  
Feedback Inhibition ◽  
Total Activity ◽  
Mitochondrial Inner Membrane ◽  
Pyruvate Oxidation ◽  
Cytochrome Pathway ◽  
Arum Maculatum ◽  
Long Chain ◽  
Sauromatum Guttatum

1. Mitochondria isolated from the thermogenic spadices of Arum maculatum and Sauromatum guttatum plants oxidized external NADH, succinate, citrate, malate, 2-oxoglutarate and pyruvate without the need to add exogenous cofactors. 2. Oxidation of substrates was virtually all via the alternative oxidase, the cytochrome pathway constituting only 10-20% of the total activity, depending on the stage of spadix development. 3. During later stages of spadix development, pyruvate oxidation was enhanced by the addition of aspartate. This was caused by acetyl-CoA condensing with oxaloacetate, produced from pyruvate/aspartate transamination, and so decreasing feedback inhibition of pyruvate dehydrogenase. 4. Pyruvate oxidation was inhibited by the long-chain acid maleimides AM5-11, but not by those with shorter polymethylene side groups, AM1-4. 5. The alpha-cyanocinnamate derivatives UK5099 [alpha-cyano-beta-(1-phenylindol-3-yl)acrylate] and CHCA [alpha-cyano-4-hydroxycinnamate] inhibited pyruvate-dependent O2 consumption and the carrier-mediated uptake of pyruvate across the mitochondrial inner membrane. Characteristics of non-competitive inhibition were observed for CHCA, whereas for UK5099 the results were more complex, suggesting a very low rate of dissociation of the inhibitor-carrier complex. 6. A comparison of the values of Vmax. and Km for oxidation and transport suggested that it was the latter which controls the overall rate of pyruvate oxidation by mitochondria from both tissues.

Immunological identification of the alternative oxidase of Neurospora crassa mitochondria

1989 ◽  
Vol 9 (3) ◽  
pp. 1362-1364
Author(s):  
A M Lambowitz ◽  
J R Sabourin ◽  
H Bertrand ◽  
R Nickels ◽  
L McIntosh
Keyword(s):  
Neurospora Crassa ◽  
Oxidase Activity ◽  
Hydroxamic Acid ◽  
Alternative Oxidase ◽  
Fungal Species ◽  
The Other ◽  
Wild Type ◽  
Higher Plant ◽  
Sauromatum Guttatum ◽  
Immunological Identification

Neurospora crassa mitochondria use a branched electron transport system in which one branch is a conventional cytochrome system and the other is an alternative cyanide-resistant, hydroxamic acid-sensitive oxidase that is induced when the cytochrome system is impaired. We used a monoclonal antibody to the alternative oxidase of the higher plant Sauromatum guttatum to identify a similar set of related polypeptides (Mr, 36,500 and 37,000) that was associated with the alternative oxidase activity of N. crassa mitochondria. These polypeptides were not present constitutively in the mitochondria of a wild-type N. crassa strain, but were produced in high amounts under conditions that induced alternative oxidase activity. Under the same conditions, mutants in the aod-1 gene, with one exception, produced apparently inactive alternative oxidase polypeptides, whereas mutants in the aod-2 gene failed to produce these polypeptides. The latter findings support the hypothesis that aod-1 is a structural gene for the alternative oxidase and that the aod-2 gene encodes a component that is required for induction of alternative oxidase activity. Finally, our results indicate that the alternative oxidase is highly conserved, even between plant and fungal species.

Purification and characterisation of recombinant DNA encoding the alternative oxidase from Sauromatum guttatum

Mitochondrion ◽  
2014 ◽  
Vol 19 ◽  
pp. 261-268 ◽  
Author(s):  
Catherine Elliott ◽  
Luke Young ◽  
Ben May ◽  
Julia Shearman ◽  
Mary S. Albury ◽  
...  
Keyword(s):  
Alternative Oxidase ◽  
Recombinant Dna ◽  
Dna Encoding ◽  
Sauromatum Guttatum

Alternative oxidase from Arum and soybean: Its stabilization during purification

1996 ◽  
Vol 96 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Qisen Zhang ◽  
Marcel H. N. Hoefnagel ◽  
Joseph T. Wiskich
Keyword(s):  
Alternative Oxidase
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