scholarly journals Structure and Possible Ureide Degrading Function of the Ubiquitous Urease of Soybean

1985 ◽  
Vol 79 (3) ◽  
pp. 794-800 ◽  
Author(s):  
Joseph C. Polacco ◽  
Roger W. Krueger ◽  
Rodney G. Winkler
Keyword(s):  
Ubiquitous Urease

scholarly journals Ubiquitous urease affects soybean susceptibility to fungi

2012 ◽  
Vol 79 (1-2) ◽  
pp. 75-87 ◽  
Author(s):  
Beatriz Wiebke-Strohm ◽  
Giancarlo Pasquali ◽  
Márcia Margis-Pinheiro ◽  
Marta Bencke ◽  
Lauro Bücker-Neto ◽  
...  
Keyword(s):  
Ubiquitous Urease

scholarly journals Soybean ubiquitous urease with purification facilitator: An addition to the moonlighting studies toolbox

2017 ◽  
Vol 53 ◽  
pp. 245-258 ◽  
Author(s):  
Anne H.S. Martinelli ◽  
Fernanda C. Lopes ◽  
Valquiria Broll ◽  
Marina S. Defferrari ◽  
Rodrigo Ligabue-Braun ◽  
...  
Keyword(s):  
Ubiquitous Urease

scholarly journals Soyuretox, an Intrinsically Disordered Polypeptide Derived from Soybean (Glycine Max) Ubiquitous Urease with Potential Use as a Biopesticide

2019 ◽  
Vol 20 (21) ◽  
pp. 5401 ◽  
Author(s):  
Karine Kappaun ◽  
Anne H. S. Martinelli ◽  
Valquiria Broll ◽  
Barbara Zambelli ◽  
Fernanda C. Lopes ◽  
...  
Keyword(s):  
Glycine Max ◽  
Plant Protection ◽  
Sodium Dodecyl ◽  
Membrane Integrity ◽  
Amino Acid Sequences ◽  
Intrinsically Disordered ◽  
Sds Micelles ◽  
Ubiquitous Urease ◽  
Insect Immune Response ◽  
Induced Aggregation

Ureases from different biological sources display non-ureolytic properties that contribute to plant defense, in addition to their classical enzymatic urea hydrolysis. Antifungal and entomotoxic effects were demonstrated for Jaburetox, an intrinsically disordered polypeptide derived from jack bean (Canavalia ensiformis) urease. Here we describe the properties of Soyuretox, a polypeptide derived from soybean (Glycine max) ubiquitous urease. Soyuretox was fungitoxic to Candida albicans, leading to the production of reactive oxygen species. Soyuretox further induced aggregation of Rhodnius prolixus hemocytes, indicating an interference on the insect immune response. No relevant toxicity of Soyuretox to zebrafish larvae was observed. These data suggest the presence of antifungal and entomotoxic portions of the amino acid sequences encompassing both Soyuretox and Jaburetox, despite their small sequence identity. Nuclear Magnetic Resonance (NMR) and circular dichroism (CD) spectroscopic data revealed that Soyuretox, in analogy with Jaburetox, possesses an intrinsic and largely disordered nature. Some folding is observed upon interaction of Soyuretox with sodium dodecyl sulfate (SDS) micelles, taken here as models for membranes. This observation suggests the possibility for this protein to modify its secondary structure upon interaction with the cells of the affected organisms, leading to alterations of membrane integrity. Altogether, Soyuretox can be considered a promising biopesticide for use in plant protection.

scholarly journals Interallelic Complementation at the Ubiquitous Urease Coding Locus of Soybean

2003 ◽  
Vol 132 (4) ◽  
pp. 1801-1810 ◽  
Author(s):  
Ariel Goldraij ◽  
Lesa J. Beamer ◽  
Joe C. Polacco
Keyword(s):  
Ubiquitous Urease ◽  
Interallelic Complementation

scholarly journals Structure-Function Insights of Jaburetox and Soyuretox: Novel Intrinsically Disordered Polypeptides Derived from Plant Ureases

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5338
Author(s):  
Matheus V. Coste Grahl ◽  
Fernanda Cortez Lopes ◽  
Anne H. Souza Martinelli ◽  
Celia R. Carlini ◽  
Leonardo L. Fruttero
Keyword(s):  
Secondary Structure ◽  
Lipid Membranes ◽  
Intrinsically Disordered Proteins ◽  
Biological Activities ◽  
3D Structure ◽  
Disordered Proteins ◽  
Recombinant Peptide ◽  
Intrinsically Disordered ◽  
Intrinsically Disordered Regions ◽  
Ubiquitous Urease

Intrinsically disordered proteins (IDPs) and intrinsically disordered regions (IDRs) do not have a stable 3D structure but still have important biological activities. Jaburetox is a recombinant peptide derived from the jack bean (Canavalia ensiformis) urease and presents entomotoxic and antimicrobial actions. The structure of Jaburetox was elucidated using nuclear magnetic resonance which reveals it is an IDP with small amounts of secondary structure. Different approaches have demonstrated that Jaburetox acquires certain folding upon interaction with lipid membranes, a characteristic commonly found in other IDPs and usually important for their biological functions. Soyuretox, a recombinant peptide derived from the soybean (Glycine max) ubiquitous urease and homologous to Jaburetox, was also characterized for its biological activities and structural properties. Soyuretox is also an IDP, presenting more secondary structure in comparison with Jaburetox and similar entomotoxic and fungitoxic effects. Moreover, Soyuretox was found to be nontoxic to zebra fish, while Jaburetox was innocuous to mice and rats. This profile of toxicity affecting detrimental species without damaging mammals or the environment qualified them to be used in biotechnological applications. Both peptides were employed to develop transgenic crops and these plants were active against insects and nematodes, unveiling their immense potentiality for field applications.

A single gene (Eu4) encodes the tissue-ubiquitous urease of soybean

1994 ◽  
Vol 242 (4) ◽  
pp. 404-414 ◽  
Author(s):  
Rebecca S. Torisky ◽  
Jeffrey D. Griffin ◽  
Richard L. Yenofsky ◽  
Joseph C. Polacco
Keyword(s):  
Single Gene ◽  
Ubiquitous Urease
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