scholarly journals Use of Zinc Ions To Study Thylakoid Protein Phosphorylation and the State 1-State 2 Transition In Vitro

1984 ◽  
Vol 74 (2) ◽  
pp. 348-354 ◽  
Author(s):  
John P. Markwell ◽  
Neil R. Baker ◽  
Michael Bradbury ◽  
J. Philip Thornber
Keyword(s):  
Protein Phosphorylation ◽  
The State ◽  
Zinc Ions ◽  
Thylakoid Protein ◽  
State 1 ◽  
Thylakoid Protein Phosphorylation

scholarly journals Evidence for different kinases in thylakoid protein phosphorylation

1987 ◽  
Vol 248 (1) ◽  
pp. 103-108 ◽  
Author(s):  
C H Foyer
Keyword(s):  
Electron Transport ◽  
Protein Kinase ◽  
Photosystem Ii ◽  
Protein Phosphorylation ◽  
System A ◽  
Intact Chloroplasts ◽  
Thylakoid Protein ◽  
Dimethyl Urea ◽  
Thylakoid Protein Phosphorylation ◽  
Differential Action

Thylakoid protein phosphorylation was facilitated in darkness by using the ferredoxin-NADPH system. CoCl2 and DBMIB (2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone) were potent inhibitors of LHCP (light-harvesting chlorophyll-binding protein) phosphorylation, but 3-(3,4-dichlorophenyl)-1,1-dimethyl-urea and atrazine had no significant effect. Differential effects on phosphorylation of the 9 kDa polypeptide and LHCP were observed in darkness with DBMIB and certain other inhibitors specific for Photosystem-II electron transport. Similarly, during illumination of intact chloroplasts or of the reconstituted chloroplast system, a differential action of bicarbonate was observed on the relative phosphorylation of the two proteins. The degree of phosphorylation of the 9 kDa polypeptide was increased in the presence of bicarbonate compared with its absence, whereas that of LHCP was relatively unchanged. Changes in the degree of phosphorylation of the 32 kDa polypeptide in these experiments did not correlate consistently with changes in phosphorylation of either LHCP or the 9 kDa polypeptide, although changes in the 32 kDa polypeptide more often paralleled phosphorylation of the 9 kDa polypeptide rather than the phosphorylation of LHCP. These observations suggest that the protein kinase that phosphorylates LHCP is distinct from that which phosphorylates the 9 kDa polypeptide.

Rapid and highly specific monitoring of reversible thylakoid protein phosphorylation by polyclonal antibody to phosphothreonine-containing proteins

2002 ◽  
Vol 159 (4) ◽  
pp. 371-377 ◽  
Author(s):  
Elena Bergo ◽  
Saijliisa Pursiheimo ◽  
Virpi Paakkarinen ◽  
Giorgio M. Giacometti ◽  
Arianna Donella-Deana ◽  
...  
Keyword(s):  
Protein Phosphorylation ◽  
Polyclonal Antibody ◽  
Thylakoid Protein ◽  
Thylakoid Protein Phosphorylation
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