scholarly journals Partial Purification and Properties of an Alkaline α-Galactosidase from Mature Leaves of Cucurbita pepo

1983 ◽  
Vol 71 (3) ◽  
pp. 662-668 ◽  
Author(s):  
Pierre-Richard Gaudreault ◽  
John A. Webb
Keyword(s):  
Cucurbita Pepo ◽  
Partial Purification ◽  
Mature Leaves ◽  
Purification And Properties

Partial purification of galactinol synthase from leaves of Cucurbita pepo

1982 ◽  
Vol 60 (7) ◽  
pp. 1054-1059 ◽  
Author(s):  
John A. Webb
Keyword(s):  
Cucurbita Pepo ◽  
Partial Purification ◽  
Galactinol Synthase ◽  
Inositol 1 ◽  
Mature Leaves ◽  
Galactosyl Transferase ◽  
Naturally Occurring ◽  
High Concentrations ◽  
Uridine Nucleotides

An enzyme synthesizing galactinol, UDP-D-galactose:myo-inositol-1-α-D-galactosyl transferase (galactinol synthase), has been isolated and partially purified from mature leaves of Cucurbita pepo. The enzyme showed optimal activity between pH 7.5 and 8.0 and required Mn2+ and the presence throughout isolation, storage, and assay of a sulfhydryl protectant (β-mercaptoethanol). EDTA was completely inhibitory. From a range of metal ions only Mg2+ partially replaced Mn2+, while Co2+, Zn2+, Cu2+, and Ni2+ were inhibitory. The uridine nucleotides and UDP-glucose were from 40 to 80% inhibitory and probably constitute part of the in vivo control system. High concentrations of galactose, melibiose, and xylose were partially inhibitory. The enzyme appeared highly specific for myo-inositol and showed no ability for galactosyl transfer to any other naturally occurring sugar or sugar alcohol. Some reactivity was obtained with the isomeric scyllo-inositol but the product was not identified. A range of other sugar nucleotides were unreactive.

Partial purification and properties of an endo-xylanase from cucumber seeds

1991 ◽  
Vol 81 (3) ◽  
pp. 327-334 ◽  
Author(s):  
Cesar V. Mujer ◽  
Dale W. Kretchman ◽  
A. Raymond Miller
Keyword(s):  
Partial Purification ◽  
Purification And Properties

Partial purification and properties of a 36-kDa 1-aminocyclopropane-l-carboxylate N-malonyltransferase from mung bean

1995 ◽  
Vol 94 (4) ◽  
pp. 629-634 ◽  
Author(s):  
Mohamed Benichou ◽  
Gracia Martinez-Reina ◽  
Felix Romojaro ◽  
Jean-Claude Pech ◽  
Alain Latche
Keyword(s):  
Mung Bean ◽  
Partial Purification ◽  
Purification And Properties

scholarly journals Partial purification and properties of a pre-mRNA splicing activity.

1985 ◽  
Vol 260 (2) ◽  
pp. 1096-1102
Author(s):  
P R DiMaria ◽  
G Kaltwasser ◽  
C J Goldenberg
Keyword(s):  
Mrna Splicing ◽  
Partial Purification ◽  
Purification And Properties

Partial Purification and Properties of Human Brain Aldehyde Dehydrogenases

1985 ◽  
Vol 45 (6) ◽  
pp. 1903-1910 ◽  
Author(s):  
Jacques-Andre Maring ◽  
Richard A. Deitrich ◽  
Roger Little
Keyword(s):  
Human Brain ◽  
Partial Purification ◽  
Aldehyde Dehydrogenases ◽  
Purification And Properties
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