scholarly journals N-Methyl Mesoporphyrin IX Inhibits Phycocyanin, but Not Chlorophyll Synthesis in Cyanidium caldarium

1983 ◽  
Vol 71 (2) ◽  
pp. 263-268 ◽  
Author(s):  
Samuel I. Beale ◽  
Nancy C. Chen
Keyword(s):  
Chlorophyll Synthesis ◽  
Cyanidium Caldarium

scholarly journals The effect of gabaculine on tetrapyrrole biosynthesis and heterotrophic growth in Cyanidium caldarium

1988 ◽  
Vol 254 (3) ◽  
pp. 907-910 ◽  
Author(s):  
J D Houghton ◽  
L Turner ◽  
S B Brown
Keyword(s):  
Chlorophyll Synthesis ◽  
Similar Rate ◽  
Red Alga ◽  
Heterotrophic Growth ◽  
Tetrapyrrole Biosynthesis ◽  
Cyanidium Caldarium ◽  
Pigment Synthesis ◽  
Subsequent Exposure ◽  
Light Inhibition ◽  
Lag Period

Pigment synthesis in four strains of the unicellular red alga Cyanidium caldarium with different pigment-synthesizing patterns was inhibited in the presence of gabaculine (3-amino-2,3-dihydrobenzoic acid). Parallel inhibition of light-induced chlorophyll and phycocyanin synthesis was observed in strain III-D-2, which only synthesizes pigments in the light. Similar parallel inhibition was observed in the dark in mutant CPD, which is able to synthesize chlorophyll and phycocyanin in the absence of light. Inhibition of pigment synthesis in all strains was overcome by addition of 5-aminolaevulinic acid. Inhibition of phycocyanin synthesis in mutant GGB (unable to synthesize chlorophyll) and inhibition of chlorophyll synthesis in mutant III-C (unable to synthesize phycocyanin) were also observed. Gabaculine also inhibited the heterotrophic growth of C. caldarium in the dark. However, inhibition was overcome after an extended lag period, following which cell growth proceeded at a similar rate to that of control cells not exposed to gabaculine. Heterotrophic growth in cells pre-exposed to gabaculine was not inhibited by subsequent exposure. Possible mechanisms for this adaptation are discussed.

Ultrastructural Localization of Phycocyanin in the Acidophilic, Thermophilic Alga, Cyanidium Caldarium

1973 ◽  
Vol 31 ◽  
pp. 462-463
Author(s):  
M. R. Edwards ◽  
J. D. Mainwaring
Keyword(s):  
Yellowstone National Park ◽  
National Park ◽  
Ultrastructural Localization ◽  
Review Of Literature ◽  
Cyanidium Caldarium ◽  
Taxonomic Rank ◽  
Thin Sections ◽  
Standard Methods ◽  
Laboratory Cultures

Although the general ultrastructure of Cyanidium caldarium, an acidophilic, thermophilic alga of questionable taxonomic rank, has been extensively studied (see review of literature in reference 1), some peculiar ultrastructural features of the chloroplast of this alga have not been noted by other investigators.Cells were collected and prepared for thin sections at the Yellowstone National Park and were also grown in laboratory cultures (45-52°C; pH 2-5). Fixation (glutaraldehyde-osmium), dehydration (ethanol), and embedding (Epon 812) were accomplished by standard methods. Replicas of frozenfracture d- etched cells were obtained in a Balzers apparatus. In addition, cells were examined after disruption in a French Press.

Effects of photoperiods and temperatures on physiological characteristics and chlorophyll synthesis precursors of adzuki bean seedlings

2019 ◽  
Vol 45 (3) ◽  
pp. 460 ◽  
Author(s):  
Ning HE ◽  
Xue-Yang WANG ◽  
Liang-Zi CAO ◽  
Da-Wei CAO ◽  
Yu LUO ◽  
...  
Keyword(s):  
Chlorophyll Synthesis ◽  
Physiological Characteristics ◽  
Adzuki Bean

Inhibition of Chlorophyll Synthesis by Pseudomonas glycinea 1

Crop Science ◽  
1979 ◽  
Vol 19 (2) ◽  
pp. 261-264 ◽  
Author(s):  
T. Gulya ◽  
J. M. Dunleavy
Keyword(s):  
Chlorophyll Synthesis

scholarly journals Studies on C-phycocyanin from Cyanidium caldarium, a eukaryote at the extremes of habitat

2000 ◽  
Vol 1456 (2-3) ◽  
pp. 99-107 ◽  
Author(s):  
Leslie E. Eisele ◽  
Sasha H. Bakhru ◽  
Xuemei Liu ◽  
Robert MacColl ◽  
Mercedes R. Edwards
Keyword(s):  
Cyanidium Caldarium

scholarly journals The Absence of Protochlorophyll(ide) Accumulation in Algae with Inhibited Chlorophyll Synthesis

1980 ◽  
Vol 65 (3) ◽  
pp. 469-471 ◽  
Author(s):  
Richard J. Ellis ◽  
Charles Timson
Keyword(s):  
Chlorophyll Synthesis

Measurement of ferrochelatase activity using a novel assay suggests that plastids are the major site of haem biosynthesis in both photosynthetic and non-photosynthetic cells of pea (Pisum sativum L.)

2002 ◽  
Vol 362 (2) ◽  
pp. 423-432 ◽  
Author(s):  
Johanna E. CORNAH ◽  
Jennifer M. ROPER ◽  
Davinder Pal SINGH ◽  
Alison G. SMITH
Keyword(s):  
Ferrous Iron ◽  
Specific Activity ◽  
Higher Plants ◽  
Protoporphyrin Ix ◽  
Chlorophyll Synthesis ◽  
Marker Enzyme ◽  
Hexane Extraction ◽  
Higher Plant ◽  
Major Site ◽  
Haem Biosynthesis

Ferrochelatase is the terminal enzyme of haem biosynthesis, catalysing the insertion of ferrous iron into the macrocycle of protoporphyrin IX, the last common intermediate of haem and chlorophyll synthesis. Its activity has been reported in both plastids and mitochondria of higher plants, but the relative amounts of the enzyme in the two organelles are unknown. Ferrochelatase is difficult to assay since ferrous iron requires strict anaerobic conditions to prevent oxidation, and in photosynthetic tissues chlorophyll interferes with the quantification of the product. Accordingly, we developed a sensitive fluorimetric assay for ferrochelatase that employs Co2+ and deuteroporphyrin in place of the natural substrates, and measures the decrease in deuteroporphyrin fluorescence. A hexane-extraction step to remove chlorophyll is included for green tissue. The assay is linear over a range of chloroplast protein concentrations, with an average specific activity of 0.68nmol·min−1·mg of protein−1, the highest yet reported. The corresponding value for mitochondria is 0.19nmol·min−1·mg of protein−1. The enzyme is inhibited by N-methylprotoporphyrin, with an estimated IC50 value of ≈ 1nM. Using this assay we have quantified ferrochelatase activity in plastids and mitochondria from green pea leaves, etiolated pea leaves and pea roots to determine the relative amounts in the two organelles. We found that, in all three tissues, greater than 90% of the activity was associated with plastids, but ferrochelatase was reproducibly detected in mitochondria, at levels greater than the contaminating plastid marker enzyme, and was latent. Our results indicate that plastids are the major site of haem biosynthesis in higher plant cells, but that mitochondria also have the capacity for haem production.

Control of chlorophyll synthesis by phytochrome

Planta ◽  
1976 ◽  
Vol 132 (3) ◽  
pp. 291-295 ◽  
Author(s):  
H. Kasemir ◽  
G. Prelim
Keyword(s):  
Chlorophyll Synthesis
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