scholarly journals Specific Determination of α-Amylase Activity in Crude Plant Extracts Containing β-Amylase

1983 ◽  
Vol 71 (2) ◽  
pp. 229-234 ◽  
Author(s):  
Douglas C. Doehlert ◽  
Stanley H. Duke
Keyword(s):  
Plant Extracts ◽  
Amylase Activity ◽  
Specific Determination

scholarly journals Total and Pancreatic Amylase Measured with 2-Chloro-4-nitrophenyl-4-O-β-d-galactopyranosylmaltoside

2000 ◽  
Vol 46 (7) ◽  
pp. 928-933 ◽  
Author(s):  
Yoshitaka Morishita ◽  
Yoshitsugu Iinuma ◽  
Nobuo Nakashima ◽  
Keiichi Majima ◽  
Katsuhiko Mizuguchi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Amylase Activity ◽  
Biological Fluids ◽  
Transfer Reactions ◽  
Pancreatic Amylase ◽  
Salivary Amylase ◽  
Specific Determination ◽  
Routine Procedures

Abstract Background: Many different methods have been used to assay amylase activity, using nitrophenylated oligosaccharides as substrate; however, the hydrolysis steps in these methods are complex. Methods: We developed a new continuously monitoring assay for amylase activity in biological fluids, using 2-chloro-4-nitrophenyl-4-O-β-d-galactopyranosylmaltoside (GalG2CNP) as the substrate; this assay was used with anti-human salivary amylase monoclonal antibodies for specific determination of the pancreatic isoenzyme. Amylase converted GalG2CNP into β-d-galactopyranosylmaltose and 2-chloro-4-nitrophenol, which was measured at 405 nm. Results: GalG2CNP was cleaved between 2-chloro-4-nitrophenol and β-d-galactopyranosylmaltose and did not undergo transfer reactions. The within-assay CVs (n = 20) for total amylase (T-AMY) and pancreatic amylase (P-AMY) were 0.6–1.6% and 0.5–2.5%, respectively; and day-to-day CVs (n = 10) for T-AMY and P-AMY were 0.8–3.7% and 0.6–4.1%, respectively. T-AMY and P-AMY activities in serum or urine obtained by the proposed method correlated well with those determined by the 2-chloro-4-nitrophenyl 4-O-β-d-galactopyranosyl-β-maltotetraoside method or the modified IFCC method. Conclusions: This novel assay for T-AMY and P-AMY measures both activities stoichiometrically, directly, and easily, and may be suitable for routine procedures.

Capillary zone electrophoretic determination of phenolic compounds in chess (Bromus inermisL.) plant extracts

2006 ◽  
Vol 29 (2) ◽  
pp. 308-313 ◽  
Author(s):  
Dagmar Štěrbová ◽  
Jiří Vlček ◽  
Vlastimil Kubáň
Keyword(s):  
Phenolic Compounds ◽  
Plant Extracts ◽  
Capillary Zone

Rapid, sensitive and specific determination of oxytetracycline residues in bovine milk and meat by CAD MIKES analysis at the 1 ppb level

1985 ◽  
Vol 12 (9) ◽  
pp. 493-496 ◽  
Author(s):  
P. Traldi ◽  
S. Daolio ◽  
B. Pelli ◽  
R. Maffei Facino ◽  
M. Carini
Keyword(s):  
Bovine Milk ◽  
Specific Determination
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