scholarly journals Plasma Membranes from Oats Prepared by Partition in an Aqueous Polymer Two-Phase System

1982 ◽  
Vol 70 (5) ◽  
pp. 1429-1435 ◽  
Author(s):  
Susanne Widell ◽  
Tomas Lundborg ◽  
Christer Larsson
Keyword(s):  
Plasma Membranes ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Polymer

scholarly journals Fractionation of rat liver plasma-membrane regions by two-phase partitioning

1986 ◽  
Vol 235 (3) ◽  
pp. 685-691 ◽  
Author(s):  
P Gierow ◽  
M Sommarin ◽  
C Larsson ◽  
B Jergil
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Plasma Membranes ◽  
Gradient Centrifugation ◽  
Fold Increase ◽  
Phase System ◽  
Two Phase ◽  
Phase Partitioning ◽  
Aqueous Polymer ◽  
Counter Current

Rat liver plasma membranes, enriched in blood-sinusoidal or bile-canalicular regions by differential and sucrose-gradient centrifugation, were further purified by partitioning in an aqueous polymer two-phase system. This method separates membranes according to differences in surface properties rather than size and density. A several-fold increase in the ratio of leucine aminopeptidase (a bile-canalicular marker) and 5′-nucleotidase to asialo-orosomucoid binding (a blood-sinusoidal marker) was obtained in one fraction, whereas another fraction gave a 2-3-fold increase in ratio of blood-sinusoidal to bile-canalicular markers. Furthermore, the markers for both regions of the plasma membrane, as well as markers for Golgi membranes and lysosomes, showed a heterogeneous behaviour on counter-current distribution.

scholarly journals Extraction and Purification of Capsaicin from Capsicum Oleoresin Using a Combination of Tunable Aqueous Polymer-Phase Impregnated Resin (TAPPIR) Extraction and Chromatography Technology

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3956 ◽  
Author(s):  
Lu ◽  
Cui
Keyword(s):  
Sodium Citrate ◽  
Extraction Process ◽  
Fractional Factorial ◽  
Phase System ◽  
Polymer Phase ◽  
Macroporous Resin ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Extraction And Purification ◽  
Extraction Step

Capsaicin, which mainly comes from pepper, exhibits anticancer, antioxidant, and anti-obesity properties. This work aims to construct a comprehensive technology for the extraction and purification of capsaicin from capsicum oleoresin. The tunable aqueous polymer phase impregnated HZ816 resins were selected in extraction step. In the extraction process, 3 g of impregnated HZ816 macroporous resin was employed per system. The results showed that a higher molecular weight of Polyethylene glycol (PEG) and 1-ethyl-3-methyl imidazolium acetate ([Emim] [OAc]) are more beneficial to the improvement of the yield of capsaicin. Screening experiment using fractional factorial designs indicated that the amount of sample loading, pH, and concentration of [Emim] [OAc] and PEG 6000 significantly affect the yield of capsaicin. Mathematical models of capsaicin yield in tunable aqueous polymer-phase impregnated resins were established and optimum condition was obtained using response surface methodology. The optimum impregnated phase was the polymer phase of an aqueous two-phase system which contained 18.5% (w/w) PEG6000, 15% (w/w) sodium citrate, and 10% (w/w) [Emim] [OAc] at pH 6.5. Under the optimal conditions, the yield of capsaicin reached 95.82% when the extraction system contains 0.25 g capsicum oleoresin. Ultimately, capsaicinoids extract was purified by reverse-phase resin (SKP-10-4300) chromatographic column. The capsaicin recovery and purity achieved 85% and 92%, respectively.

PREPARATION AND CHARACTERIZATION OF INTACT AND FREE SPORES OF BACILLUS POPILLIAE DUTKY

1967 ◽  
Vol 13 (3) ◽  
pp. 279-285 ◽  
Author(s):  
Grant St. Julian ◽  
Thomas G. Pridham ◽  
Harlow H. Hall
Keyword(s):  
Malachite Green ◽  
Potassium Phosphate ◽  
Staining Technique ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Sonic Treatment ◽  
Bacillus Popilliae ◽  
Almost All

Spores of Bacillus popilliae are enclosed within a large sporangium. In an effort to characterize the spores precisely, a method was developed to free them from their sporangia by sonic vibration. Separation of the free spores from sporangial debris was made by lysozyme treatment, centrifugation, and the Sacks and Alderton aqueous polymer two-phase system. The intact spores are fully refractile; they accept and retain malachite green applied by a modified Wirtz spore-staining technique. The free spores are elongated with rounded ends and measure 1.30–2.25 μ × 0.90–1.40 μ. Outgrowth of intact germinated spores is lowest when heat-activated at 80 °C and highest when heat-activated at 50 °C. Free spores are more heat sensitive than are intact spores, possibly because of damage by the sonic treatment. L-Alanine, adenosine, and glucose separately and in combination do not influence germination of either intact or free spores. Although potassium phosphate somewhat increased germination of intact spores, other salts were ineffective, but almost all salts increased germination of free spores.

A Device for Countercurrent Distribution of Particles by an Aqueous Polymer Two-Phase System

1978 ◽  
Vol 13 (9) ◽  
pp. 745-752 ◽  
Author(s):  
Hiroyuki Nakazawa ◽  
Takenori Tanimura ◽  
Zenzo Tamura
Keyword(s):  
Countercurrent Distribution ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Polymer

Purification of glucosyltransferase from cell-lysate of Streptococcus mutans by counter-current chromatography using aqueous polymer two-phase system

2004 ◽  
Vol 805 (1) ◽  
pp. 155-160 ◽  
Author(s):  
Akio Yanagida ◽  
Mitsuhiro Isozaki ◽  
Yoichi Shibusawa ◽  
Heisaburo Shindo ◽  
Yoichiro Ito
Keyword(s):  
Streptococcus Mutans ◽  
Phase System ◽  
Two Phase ◽  
Cell Lysate ◽  
Aqueous Polymer ◽  
Counter Current

Concentration of Poliovirus by an Aqueous Polymer Two-Phase System

Nature ◽  
1960 ◽  
Vol 188 (4755) ◽  
pp. 1047-1048 ◽  
Author(s):  
E. C. J. NORRBY ◽  
P. Å. ALBERTSSON
Keyword(s):  
Phase System ◽  
Two Phase ◽  
Aqueous Polymer

scholarly journals Comparative studies on the soluble and plasma membrane associated nitrate reductase from Cucumis sativus L.

2014 ◽  
Vol 63 (3-4) ◽  
pp. 309-314 ◽  
Author(s):  
Grażyna Kłobus ◽  
Jolanta Marciniak ◽  
Józef Buczek
Keyword(s):  
Plasma Membrane ◽  
Nitrate Reductase ◽  
Plasma Membranes ◽  
Polyclonal Antiserum ◽  
Soluble Form ◽  
Phase System ◽  
Two Phase ◽  
Cucumis Sativus L ◽  
Cucumber Roots ◽  
Biochemical Comparison

The biochemical comparison between two forms of nitrate reductase from cucumber roots: the soluble enzyme and the plasma membrane-associated one was made. Soluble nitrate reductase was purified on the blue-Sepharose 4B. The nitrate reductase bound with plasma membranes was isolated from cucumber roots by partition of microsomes in the 6.5% dextran-PEG two phase system. The molecular weight of native enzyme estimated with HPLC was 240 kDa and 114 kDa for the soluble and membrane bounded enzyme, respectively. Temperature induced phase separation in Triton X-114 indicated a huge difference in hydrophobicity of the plasma membrane associated nitrate reductase and soluble form of enzyme. Small differences were observed in partial activities of plasma membrane nitrate reductase and soluble nitrate reductase. Also experiments with polyclonal antiserum raised against the native nitrate reductase showed some differences in the immunological properties of both forms of the nitrate reductase. The above results indicated that in cucumber roots two different forms of the nitrate reductase are present.

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