scholarly journals Regulation of Cytoplasmic and Vacuolar pH in Maize Root Tips under Different Experimental Conditions

1982 ◽  
Vol 69 (6) ◽  
pp. 1344-1347 ◽  
Author(s):  
Justin K. M. Roberts ◽  
David Wemmer ◽  
Peter M. Ray ◽  
Oleg Jardetzky
Keyword(s):  
Maize Root ◽  
Root Tips ◽  
Experimental Conditions ◽  
Vacuolar Ph

scholarly journals The Endoplasmic Reticulum and the Golgi Structures in Maize Root Cells

1959 ◽  
Vol 5 (3) ◽  
pp. 501-506 ◽  
Author(s):  
W. Gordon Whaley ◽  
Hilton H. Mollenhauer ◽  
Joyce E. Kephart
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Wall ◽  
Electron Microscope ◽  
Nuclear Envelope ◽  
Epoxy Resin ◽  
Maize Root ◽  
Root Tips ◽  
Animal Cells ◽  
Root Cells ◽  
Vesicular Structure

Maize root tips were fixed in potassium permanganate, embedded in epoxy resin, sectioned to show silver interference color, and studied with the electron microscope. All the cells were seen to contain an endoplasmic reticulum and apparently independent Golgi structures. The endoplasmic reticulum is demonstrated as a membrane-bounded, vesicular structure comparable in many aspects to that of several types of animal cells. With the treatment used here the membranes appear smooth surfaced. The endoplasmic reticulum is continuous with the nuclear envelope and, by contact at least, with structures passing through the cell wall. The nuclear envelope is characterized by discontinuities, as previously reported for animal cells. The reticula of adjacent cells seem to be in contact at or through the plasmodesmata. Because of these contacts the endoplasmic reticulum of a given cell appears to be part of an intercellular system. The Golgi structures appear as stacks of platelet-vesicles which apparently may, under certain conditions, produce small vesicles around their edges. Their form changes markedly with development of the cell.

Metabolism of proline in maize root tips

1970 ◽  
Vol 48 (6) ◽  
pp. 1155-1158 ◽  
Author(s):  
Ruth A. Barnard ◽  
Ann Oaks
Keyword(s):  
Incubation Period ◽  
Maize Root ◽  
Root Tips ◽  
Intact Roots

During an initial 3-h incubation period, maize root tips loose 62% of their soluble proline. When intact roots are presented with proline-UL-14C for 30 min and subsequently excised, 80% of that proline is used in 3 h. Sixty-six percent of the convertible proline is oxidized to glutamic, malic, succinic, and citric acids, 34% is incorporated into protein as proline during the initial 30-min pulse.

Substrate cycles in the central metabolism of maize root tips under hypoxia

2007 ◽  
Vol 68 (16-18) ◽  
pp. 2222-2231 ◽  
Author(s):  
Ana Paula Alonso ◽  
Philippe Raymond ◽  
Dominique Rolin ◽  
Martine Dieuaide-Noubhani
Keyword(s):  
Maize Root ◽  
Central Metabolism ◽  
Root Tips ◽  
Substrate Cycles

The influence of nitrate on the induction of nitrate reductase in maize roots

1973 ◽  
Vol 51 (6) ◽  
pp. 1255-1258 ◽  
Author(s):  
D. L. Stevens ◽  
Ann Oaks
Keyword(s):  
Nitrate Reductase ◽  
Maize Root ◽  
Lag Phase ◽  
Root Tips ◽  
Final Level ◽  
Maize Roots ◽  
Mature Root

A concentration of 10 mM NO3 saturates the induction of nitrate reductase in maize root tips whereas concentrations up to 100 mM do not saturate the induction in mature root sections. Increasing concentrations of nitrate from 1 to 25 mM have no effect on either the lag phase, or the phase of rapid increase of the enzyme. They do influence the final level of enzyme obtained at 8 h.

Cell synchronization and isolation of metaphase chromosomes from maize (Zea mays L.) root tips for flow cytometric analysis and sorting

Genome ◽  
1996 ◽  
Vol 39 (4) ◽  
pp. 697-703 ◽  
Author(s):  
Jai-Heon Lee ◽  
K. Arumuganathan ◽  
S. M. Kaeppler ◽  
C. M. Papa ◽  
H. F. Kaeppler
Keyword(s):  
Cell Cycle ◽  
Flow Cytometric Analysis ◽  
Maize Root ◽  
Root Tip ◽  
Root Tips ◽  
Chromosome Sorting ◽  
Synthesis Inhibitor ◽  
Metaphase Chromosomes ◽  
Flow Karyotype ◽  
Flow Cytometric

Accumulation of cells containing metaphase chromosomes is an important step in cytological analyses and chromosome sorting procedures. The goal of this research was to optimize treatment parameters to synchronize the cell cycle of maize root tip meristem cells. Levels of hydroxyurea, a DNA synthesis inhibitor, were assessed for their utility in accumulating cells at the G1 phase of the cell cycle. Trifluralin, amiprophos-methyl, and colchicine were used to accumulate cells containing metaphase chromosomes upon release from hydroxyurea inhibition. Optimal mitotic indices were achieved by treating seedlings with 5 mM hydroxyurea for 18 h, incubating for 1 h without chemical treatment to release the hydroxyurea block, and then treating emerging roots with 1 μM trifluralin for 4 h. The mitotic index of synchronized maize root tips was over 70%. Uniformity of synchronization depended upon selection of seeds with emerging radicles that were similar in length at the time of treatment. Suspensions of intact chromosomes were prepared by a simple slicing procedure. The chromosome preparations were found to be suitable for flow cytometric characterization and sorting. Chromosome peaks of the observed flow karyotype resembled the predicted flow karyotype calculated on the basis of maize chromosome size. Key words : flow karyotype, hydroxyurea, plant chromosome sorting, trifluralin.

scholarly journals Regulation of Protein Degradation and Protease Expression by Mannose in Maize Root Tips. Pi Sequestration by Mannose May Hinder the Study of Its Signaling Properties

2001 ◽  
Vol 125 (3) ◽  
pp. 1485-1498 ◽  
Author(s):  
Renaud Brouquisse ◽  
Adeline Evrard ◽  
Dominique Rolin ◽  
Philippe Raymond ◽  
Claude Roby
Keyword(s):  
Protein Degradation ◽  
Maize Root ◽  
Root Tips ◽  
Protease Expression
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