scholarly journals In Vivo Nitrite Reduction in Leaf Tissue of Phaseolus vulgaris L.

1981 ◽  
Vol 68 (5) ◽  
pp. 1068-1072 ◽  
Author(s):  
David R. Peirson ◽  
J. Richard Elliott
2019 ◽  
Vol 20 (9) ◽  
pp. 2327 ◽  
Author(s):  
Marcello Iriti ◽  
Alessio Scarafoni ◽  
Simon Pierce ◽  
Giulia Castorina ◽  
Sara Vitalini

EM (effective microorganisms) is a biofertilizer consisting of a mixed culture of potentially beneficial microorganisms. In this study, we investigated the effects of EM treatment on leaf in vivo chlorophyll a fluorescence of photosystem II (PSII), yield, and macronutrient content of bean plants grown on different substrates (nutrient rich substrate vs. nutrient poor sandy soil) in controlled environmental conditions (pot experiment in greenhouse). EM-treated plants maintained optimum leaf photosynthetic efficiency two weeks longer than the control plants, and increased yield independent of substrate. The levels of seed nutritionally-relevant molecules (proteins, lipids, and starch) were only slightly modified, apart from the protein content, which increased in plants grown in sandy soil. Although EM can be considered a promising and environmentally friendly technology for sustainable agriculture, more studies are needed to elucidate the mechanism(s) of action of EM, as well as its efficacy under open field conditions.


Hoehnea ◽  
2011 ◽  
Vol 38 (2) ◽  
pp. 273-280 ◽  
Author(s):  
Carlos Pimentel ◽  
Ricardo Ferraz de Oliveira ◽  
Rafael Vasconcelos Ribeiro ◽  
Mauro Guida dos Santos ◽  
Eduardo Caruso Machado

A análise in vivo da taxa de assimilação líquida de CO2 (A) em resposta à densidade de fluxo de fótons fotossinteticamente ativos (DFFFA) e em resposta à concentração intercelular de CO2 da folha (Ci) permite uma avaliação detalhada do processo fotossintético. Com as curvas A/DFFFA, se estabelece a DFFFA máxima, em que a maior parte da energia absorvida é transferida para a formação de produtos fotoquímicos. Já com as curvas A/Ci, calculou-se a velocidade máxima de carboxilação da Rubisco (Vc,max) de 89,3 µmol m-2 s-1, a velocidade máxima de oxigenação da Rubisco (Vo,max) de 24,7 µmol m-2 s-1, a velocidade máxima de transporte de elétrons (Jmax) de 159,8 µmol m-2 s-1, do qual a regeneração de RuBP é dependente, a respiração mitocondrial (Rd) de 0,88 µmol m-2 s-1, e a condutância mesofílica (g m) de 0,47 mol m-2 s-1, para o feijoeiro comum cultivado no Brasil (Phaseolus vulgaris var. mexicanus).


2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Ivan Luzardo ◽  
Rocio Campos-Vega ◽  
Elvira Gonzalez de Mejia ◽  
Flavia Loarca

Abstract Objectives The aim of this research was to evaluate the anti-inflammatory properties of an oven-baked nixtamalized corn (Zea mays L.)/cooked common bean (Phaseolus vulgaris L.) chip in a model of chronic colitis in vivo. The hypothesis was that the chip consumption would prevent colon barrier disruption and improve enzymatic biomarkers by the modulation of infiltration and adhesion of inflammatory cells. Methods A 70% corn and 30% bean chip (7030C) was evaluated, using dextran sodium sulfate (DSS, 2% v/v) as chemical inductor of colitis. After 1-week of acclimatization, 36 CD-1 male mice (6–8 weeks age) were randomly divided into 5 groups: G1 (negative control, fed with basal diet, BD, and water); G2 (positive control, BD + DSS), G3 (100 g 7030C/kg body weight, BW/day + BD), G4 (200 g 7030C/kg BW + BD) and G5 (300 g 7030C/kg BW + BS). The G2-G5 groups were administered DSS every other week, during 5-weeks.After the mice were euthanized, BW and disease activity index (DAI) were recorded. Liver, colon, and spleen were collected, weighed and analyzed for histology. Colonic myeloperoxidase (MPO)/fecal b-glucuronidase (GLUC) activities were also quantified, as well as fecal/cecal metabolites. The colonic mRNA expression of inflammation-associated genes was conducted using a gene inflammation profiler array. Results DSS increased DAI up to 2 units, BW loss was 10–17%, and induced colon shortening 10–15%. Compared to G2, G4 exhibited significantly (p < 0.05) lower DAI (0.75 ± 0.01), spleen relative weight (0.003 ± 0.0001) and colon weight/length ratio (0.045 ± 0.008). The histological analysis showed that the chip consumption prevented colonic barrier damage. G4 displayed the lowest MPO and GLUC among all DSS-induced groups (0.004 ± 0.0004 mU/mg colon; 0.44 ± 0.01 mmol/min/g feces, respectively), and the lowest seric content of MCP-1 protein. Amid all the quantified metabolites, the chip consumption significantly reduced the fecal/cecal content of acetic acid, while butyric and propionic increased at the end of the study. Inflammation gene expression was modulated by the chip consumption. Conclusions Our results suggest that the consumption of this chip might alleviate chronic colitis symptoms because of a protective effect in the gut barrier function and the modulation of infiltration of inflammatory cells. Funding Sources The funding received by CONACyT, CONCyTEQ-Mexico and NIFA-USDA-HATCH are appreciated.


2013 ◽  
Vol 91 ◽  
pp. 84-89 ◽  
Author(s):  
Carlos Pimentel ◽  
Rafael V. Ribeiro ◽  
Eduardo C. Machado ◽  
Mauro G. dos Santos ◽  
Ricardo F. de Oliveira

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Jason Wiesinger ◽  
Raymond Glahn ◽  
Karen Cichy ◽  
Nikolai Kolba ◽  
Jon Hart ◽  
...  

Abstract Objectives The common dry bean (Phaseolus vulgaris L.) is a globally produced pulse crop and an important source of protein and micronutrients for millions of people across Latin America and Africa. In these regions, energy for cooking is expensive or scarce and long cooking times deter consumers from purchasing beans. In addition, many of the preferred black and red seed types have phytate and polyphenols that limit the absorption of trace minerals. Yellow beans are unique because their seed coats are rich in kaempferol 3-glucoside, a recently discovered promoter of iron absorption. Several market classes of yellow beans are sold throughout Latin America and Africa, where they are marketed at premium prices for their fast cooking tendencies. Exploring the yellow bean's unique heritage to develop new fast cooking varieties that deliver more absorbable iron would be useful for regions where inhabitants have limited access to fuelwood for cooking. This study compared the iron bioavailability of three fast cooking yellow beans from Africa with contrasting seed coat colors (Manteca, Amarillo, Njano) to slower cooking white and red kidney commercial varieties from North America (Table 1). Methods Cooked beans were formulated into diets with the complementary food crops of potato, rice and cabbage. Iron bioavailability was measured as ferritin formation in an in vitro digestion Caco-2 bioassay and the ability to maintain total body iron hemoglobin (Hb-Fe) during a 6 week in vivo (Gallus gallus) feeding trial. Results Animals fed yellow bean diets had faster growth rates, accumulated more dietary iron and had higher Hb-Fe than animals fed either kidney bean diet (Figure 1). In contrast to yellow beans, the kidney beans had almost no kaempferol 3-glucoside (Table 2). When compared to the other four bean based diets, the fast cooking Manteca yellow bean diet had the highest Caco-2 ferritin formation in vitro (Table 3) and delivered the largest increase in Hb-Fe in vivo (Figure 1). Conclusions Through the added benefit of fast preparation times and improved iron quality after cooking, this study provides evidence that the Manteca market class is worthy of germplasm enhancement as a new convenience food to help alleviate trace mineral deficiencies in regions where beans are widely accepted as a dietary staple. Funding Sources USDA-NIFA. Supporting Tables, Images and/or Graphs


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