scholarly journals Evidence for Independent Genetic Control of the Multiple Forms of Maize Endosperm Branching Enzymes and Starch Synthases

1981 ◽  
Vol 67 (6) ◽  
pp. 1141-1145 ◽  
Author(s):  
Charles D. Boyer ◽  
Jack Preiss
Keyword(s):  
Genetic Control ◽  
Multiple Forms ◽  
Maize Endosperm ◽  
Starch Synthases ◽  
Branching Enzymes

scholarly journals Independent Genetic Control of Maize Starch-Branching Enzymes IIa and IIb (Isolation and Characterization of a Sbe2a cDNA)

1997 ◽  
Vol 114 (1) ◽  
pp. 69-78 ◽  
Author(s):  
M. Gao ◽  
D. K. Fisher ◽  
K. N. Kim ◽  
J. C. Shannon ◽  
M. J. Guiltinan
Keyword(s):  
Genetic Control ◽  
Maize Starch ◽  
Isolation And Characterization ◽  
Starch Branching Enzymes ◽  
Branching Enzymes

Starch synthases and starch branching enzymes from Pisum sativum

1981 ◽  
Vol 20 (8) ◽  
pp. 1805-1809 ◽  
Author(s):  
Gail L. Matters ◽  
Charles D. Boyer
Keyword(s):  
Pisum Sativum ◽  
Starch Synthases ◽  
Starch Branching Enzymes ◽  
Branching Enzymes

scholarly journals Maize endosperm-specific transcription factors O2 and PBF network the regulation of protein and starch synthesis

2016 ◽  
Vol 113 (39) ◽  
pp. 10842-10847 ◽  
Author(s):  
Zhiyong Zhang ◽  
Xixi Zheng ◽  
Jun Yang ◽  
Joachim Messing ◽  
Yongrui Wu
Keyword(s):  
Transcription Factors ◽  
Double Mutant ◽  
Starch Content ◽  
Starch Synthesis ◽  
Starch Synthase ◽  
Maize Endosperm ◽  
Protein Levels ◽  
Pyruvate Orthophosphate Dikinase ◽  
Branching Enzymes ◽  
Critical Components

The maize endosperm-specific transcription factors opaque2 (O2) and prolamine-box binding factor (PBF) regulate storage protein zein genes. We show that they also control starch synthesis. The starch content in the PbfRNAi and o2 mutants was reduced by ∼5% and 11%, respectively, compared with normal genotypes. In the double-mutant PbfRNAi;o2, starch was decreased by 25%. Transcriptome analysis reveals that >1,000 genes were affected in each of the two mutants and in the double mutant; these genes were mainly enriched in sugar and protein metabolism. Pyruvate orthophosphate dikinase 1 and 2 (PPDKs) and starch synthase III (SSIII) are critical components in the starch biosynthetic enzyme complex. The expression of PPDK1, PPDK2, and SSIII and their protein levels are further reduced in the double mutants as compared with the single mutants. When the promoters of these genes were analyzed, we found a prolamine box and an O2 box that can be additively transactivated by PBF and O2. Starch synthase IIa (SSIIa, encoding another starch synthase for amylopectin) and starch branching enzyme 1 (SBEI, encoding one of the two main starch branching enzymes) are not directly regulated by PBF and O2, but their protein levels are significantly decreased in the o2 mutant and are further decreased in the double mutant, indicating that o2 and PbfRNAi may affect the levels of some other transcription factor(s) or mRNA regulatory factor(s) that in turn would affect the transcript and protein levels of SSIIa and SBEI. These findings show that three important traits—nutritional quality, calories, and yield—are linked through the same transcription factors.

Sign in / Sign up

Export Citation Format

Share Document