scholarly journals Isolation and Characterization of Concanavalin A-labeled Plasma Membranes of Carrot Protoplasts

1979 ◽  
Vol 64 (6) ◽  
pp. 1005-1011 ◽  
Author(s):  
Wendy F. Boss ◽  
Albert W. Ruesink
Keyword(s):  
Concanavalin A ◽  
Plasma Membranes ◽  
Isolation And Characterization ◽  
Carrot Protoplasts

Isolation and characterization of concanavalin A polypeptide chains

Biochemistry ◽  
1971 ◽  
Vol 10 (19) ◽  
pp. 3554-3559 ◽  
Author(s):  
Allen B. Edmundson ◽  
Kathryn R. Ely ◽  
Dayle A. Sly ◽  
Florence A. Westholm ◽  
Dennis A. Powers ◽  
...  
Keyword(s):  
Concanavalin A ◽  
Isolation And Characterization ◽  
Polypeptide Chains

scholarly journals Isolation and characterization of rabbit kidney brush borders

1972 ◽  
Vol 128 (5) ◽  
pp. 1319-1328 ◽  
Author(s):  
S. J. Quirk ◽  
G. B. Robinson
Keyword(s):  
Plasma Membranes ◽  
Sucrose Density Gradient ◽  
Molar Ratio ◽  
Rabbit Kidney ◽  
Kidney Cortex ◽  
Marker Enzymes ◽  
Isolation And Characterization ◽  
Adenosine Triphosphatases ◽  
Brush Borders

1. Brush borders were isolated from rabbit kidney-cortex homogenates by rate-zonal centrifugation through a sucrose density gradient in a B-XIV zonal rotor, followed by differential centrifugation. 2. The method of preparation gave brush borders of high purity with a reasonable yield. The morphological appearance supported the evidence from enzymic and chemical investigations, that the brush borders were only slightly contaminated with endoplasmic reticulum, mitochondria, lysosomes and nuclei. 3. The molar ratio of cholesterol to phospholipid lay within the range found in other plasma membranes, but the carbohydrate content was double that found in liver plasma membranes. 4. Alkaline phosphatase, maltase, trehalase and aminopeptidase were major enzymic constituents of the brush borders, and had an approximately equal yield and enrichment, but none of these enzymes fulfilled the criteria for marker enzymes. 5. Mg2+-dependent and Na+,K+-dependent adenosine triphosphatases, although found in brush borders, had low yields and low enrichments.

Inferferon induced in human leukocytes by concanavalin A: isolation and characterization of γ- and β-type components

1981 ◽  
Vol 11 (11) ◽  
pp. 937-942 ◽  
Author(s):  
Jozef van Damme ◽  
Marc De Ley ◽  
Hendrik Claeys ◽  
Alfons Billiau ◽  
Carl Vermylen ◽  
...  
Keyword(s):  
Concanavalin A ◽  
Human Leukocytes ◽  
Isolation And Characterization
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