scholarly journals Role of Indole-3-acetic Acid and Gibberellin in the Control of Internodal Elongation in Avena Stem Segments

1978 ◽  
Vol 62 (5) ◽  
pp. 807-811 ◽  
Author(s):  
Edward N. Rapoport ◽  
Keith E. Heller ◽  
P. Dayanandan ◽  
Frederick V. Hebard ◽  
Peter B. Kaufman
Keyword(s):  
Acetic Acid ◽  
Internodal Elongation ◽  
Indole 3 Acetic Acid ◽  
Stem Segments

Role of Indole-3-Acetic Acid in NAFLD Amelioration After Sleeve Gastrectomy

2021 ◽  
Author(s):  
Yu Wang ◽  
Geng Wang ◽  
Jie Bai ◽  
Ning Zhao ◽  
Qingbo Wang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Sleeve Gastrectomy ◽  
Indole 3 Acetic Acid

scholarly journals Role of Growth Regulators on In Vitro Callus Induction and Direct Regeneration in Physalis minima Linn

2015 ◽  
Vol 44 ◽  
pp. 38-44 ◽  
Author(s):  
H. Sandhya ◽  
Rao Srinath
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Basal Medium ◽  
Direct Regeneration ◽  
Naphthalene Acetic Acid ◽  
Stem Explants ◽  
Dichlorophenoxy Acetic Acid ◽  
Indole 3 Acetic Acid

Suitable protocol for induction of callus and regeneration was developed from different explants viz., node, stem and leaves in Physalis minima. MS basal medium supplemented with various concentrations (1.0-4.0mg/l) of auxins like 2,4-Dichlorophenoxy acetic acid (2,4-D), α-naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinins (0.5-1.5mg/l) like BAP or Kn were used. All the three explants responded for induction of callus, however stem explants were found superior, followed by node and leaf. Callus induction was observed in all the auxins and combination of growth regulators used with varied mass (2010±1.10) and highest percentage of callus induction was observed from stem at 2.0mg/l 2,4-D (90%) followed by NAA (70%) and IAA (50%). Organogenesis was induced when nodal explants were transferred on MS medium supplemented with 2,4-D and Kn at various concentrations, maximum being on 2.0mg/l 2,4-D + 1.0mg/l Kn (90%). Regenerated shoots were elongated on 0.5mg/l GA3. The shoots were subsequently rooted on MS + 1.0mg/l IBA (95%) medium. Rooted shoots were hardened and acclimatized, later they were transferred to polycups containing soil, cocopeat and sand in the ratio 1:2:1.Keywords:Physalis minima, Node, Stem, Leaf, callus and growth regulators.

scholarly journals Effects of Variations in Hormonal Treatments Upon In vitro Regeneration Potential in Embryo Explants of Arenga pinnata

2021 ◽  
Vol 17 (5) ◽  
pp. 495-503
Author(s):  
Shamsiah Abdullah ◽  
Siti Nurain Roslan
Keyword(s):  
Acetic Acid ◽  
In Vitro Regeneration ◽  
Hormonal Treatment ◽  
Height Increment ◽  
Regeneration Potential ◽  
In Vitro Method ◽  
Indole 3 Acetic Acid ◽  
Hormonal Treatments

One of the challenges related to propagation of Arenga pinnata is its lengthy period of seed dormancy. In this study, in vitro regeneration was carried out to determine the effect of hormonal treatment on the embryo explant of Arenga pinnata. Embryos were surface sterilized and cultured into different media supplemented with various hormones concentrations and combinations. Each treatment contained of Kinetin (KN) hormone (1.0, 2.0, and 3.0 mg/l) and in combination with indole-3-acetic acid (IAA) of 0.1, 0.2, 0.3 mg/l. The height of plumule and length of radical was observed and recorded. Treatment 8 (3 mg/ml KN + 0.1 mg/ml IAA) showed 59.09% in plumule height increment while treatment 4 (1 mg/ml KN + 0.3 mg/ml IAA) showed the highest radical increments with 93.62%. The knowledge gained in this study consequently helps us to better understand the role of KN and IAA in the in vitro regeneration protocol. Since in vitro method able to produce higher number of in vitro seedlings at one time, it is important to establish the in vitro regeneration protocol for this plant.

scholarly journals The Role of Indole-3-Acetic Acid and Acid Invertase in the Development of Melon (Cucumis melo L. cv. Prince) Fruit

1997 ◽  
Vol 65 (4) ◽  
pp. 723-729 ◽  
Author(s):  
Tae-Ho Lee ◽  
Tohru Kato ◽  
Yoshinori Kanayama ◽  
Hajime Ohno ◽  
Kiyotoshi Takeno ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Cucumis Melo ◽  
Acid Invertase ◽  
Cucumis Melo L ◽  
Indole 3 Acetic Acid

scholarly journals Effects of sugars on the growth and chlorophyll content in excised tulip stem in the presence of Indole-3-acetic acid

2012 ◽  
Vol 20 (1) ◽  
pp. 97-114 ◽  
Author(s):  
Elżbieta Węgrzynowicz-Lesiak ◽  
Marian Saniewski ◽  
Justyna Góraj ◽  
Marcin Horbowicz ◽  
Kensuke Miyamoto ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Chlorophyll Content ◽  
Inhibitory Effect ◽  
Flower Bud ◽  
Chlorophyll Contents ◽  
Delayed Senescence ◽  
Sugar Response ◽  
Tulip Bulbs ◽  
Indole 3 Acetic Acid ◽  
Stem Segments

ABSTRACT The purpose of this study was to clarify the effect of sucrose on auxin-induced growth of stem excised from growing tulips and excised directly from cooled and not cooled bulbs, and on the growth of excised IV internode from growing plants in the presence of auxin. In all cases flower bud was replaced by IAA (indole-3- acetic acid, 0.1%, w/w in lanolin) and basal part of excised segments of stem was kept in distilled water or in solution of various sugars at different concentrations. IAA-induced growth of excised stems isolated from growing tulips was inhibited by sucrose at concentrations of 5.0% and 10.0%, but sucrose at 1.25% and 2.5% did not. Sucrose at all concentrations used evidently delayed senescence and increased chlorophyll contents in excised stems in the presence of IAA. Sucrose induced stiffing in isolated stems in the presence of IAA, and much less infective by pathogen in comparison to stem treated with IAA only. Mannitol and sorbitol at concentrations of 5.0% and 10.0% substantially inhibited IAA-induced growth of stem segments. Stem segments excised from cooled and not cooled tulip bulbs were more sensitive than those isolated from growing shoots due to application of sucrose and glucose; more inhibitory effect was observed. Sucrose at concentrations of 5.0% and 10.0% only slightly inhibited growth of IV internode treated with IAA and all concentrations of sucrose (1.25%, 2.5%, 5.0% and 10.0%) substantially increased chlorophyll content. The possible mode of actions of sucrose interacting with auxin to regulate stem growth is also discussed although sugar response is complicated by the fact that plants have multiple sugar-response pathways.

The Role of Horseradish Peroxidase in Indole-3-acetic Acid Oxidation*

Biochemistry ◽  
1965 ◽  
Vol 4 (12) ◽  
pp. 2754-2763 ◽  
Author(s):  
L. Raymond Fox ◽  
William K. Purves ◽  
Henry I. Nakada
Keyword(s):  
Acetic Acid ◽  
Horseradish Peroxidase ◽  
Acid Oxidation ◽  
Indole 3 Acetic Acid

scholarly journals The acropetal effects of indole-3-acetic acid in isolated shoot segments of Acer pseudoplatanus L. II. Possible regulation by a vectorial fieid of auxin waves

2014 ◽  
Vol 60 (1-2) ◽  
pp. 51-66
Author(s):  
Jacek A. Adamczyk
Keyword(s):  
Acetic Acid ◽  
Phase Shift ◽  
Direct Action ◽  
Lateral Branch ◽  
Stem Segment ◽  
Acer Pseudoplatanus ◽  
Basipetal Transport ◽  
Indole 3 Acetic Acid ◽  
Stem Segments ◽  
Growing Shoot

The acropetal effects of auxin on elongation of axillary buds and on modulation of the wave-like pattern of basipetal efflux of natural auxin to agar from <i>Acer pseudoplatanus</i> L. shoots were studied. When synthetic IAA was applied to cut surfaces of one of two branches the elongation growth of buds situated on the opposite branch was retarded, suggesting regulation independent of the direct action of the molecules of the applied IAA. Oscillations in basipetal transport of natural auxin along the stem segments were observed corroborating the results of other authors using different tree species. Apical application of synthetic IAA for 1 hour to the lateral branch caused a phase shift of the wave-like pattern of basipetal efflux of natural auxin, when the stem segment above the treated branch was sectioned. The same effect was observed evoked by the laterally growing branch which is interpreted as an effect of natural auxin produced by the actively growing shoot. These modulations could be propagated acropetally at a rate excluding direct action of auxin molecules at the sites of measurement. The results seem to corroborate the hypothesis suggesting that auxin is involved in acropetal regulation of shoot apex growth through its effect upon modulation of the vectorial field which arises when the auxin-waves translocate in cambium.

scholarly journals iac Gene Expression in the Indole-3-Acetic Acid-Degrading Soil Bacterium Enterobacter soli LF7

2018 ◽  
Vol 84 (19) ◽  
Author(s):  
Isaac V. Greenhut ◽  
Beryl L. Slezak ◽  
Johan H. J. Leveau
Keyword(s):  
Gene Expression ◽  
Acetic Acid ◽  
Promoter Region ◽  
Deletion Mutant ◽  
Plant Hormone ◽  
Major Facilitator Superfamily ◽  
Soil Bacterium ◽  
Content Type ◽  
Indole 3 Acetic Acid

ABSTRACT We show for soil bacterium Enterobacter soli LF7 that the possession of an indole-3-acetic acid catabolic (iac) gene cluster is causatively linked to the ability to utilize the plant hormone indole-3-acetic acid (IAA) as a carbon and energy source. Genome-wide transcriptional profiling by mRNA sequencing revealed that these iac genes, chromosomally arranged as iacHABICDEFG and coding for the transformation of IAA to catechol, were the most highly induced (>29-fold) among the relatively few (<1%) differentially expressed genes in response to IAA. Also highly induced and immediately downstream of the iac cluster were genes for a major facilitator superfamily protein (mfs) and enzymes of the β-ketoadipate pathway (pcaIJD-catBCA), which channels catechol into central metabolism. This entire iacHABICDEFG-mfs-pcaIJD-catBCA gene set was constitutively expressed in an iacR deletion mutant, confirming the role of iacR, annotated as coding for a MarR-type regulator and located upstream of iacH, as a repressor of iac gene expression. In E. soli LF7 carrying the DNA region upstream of iacH fused to a promoterless gfp gene, green fluorescence accumulated in response to IAA at concentrations as low as 1.6 μM. The iacH promoter region also responded to chlorinated IAA, but not other aromatics tested, indicating a narrow substrate specificity. In an iacR deletion mutant, gfp expression from the iacH promoter region was constitutive, consistent with the predicted role of iacR as a repressor. A deletion analysis revealed putative −35/−10 promoter sequences upstream of iacH, as well as a possible binding site for the IacR repressor. IMPORTANCE Bacterial iac genes code for the enzymatic conversion of the plant hormone indole-3-acetic acid (IAA) to catechol. Here, we demonstrate that the iac genes of soil bacterium Enterobacter soli LF7 enable growth on IAA by coarrangement and coexpression with a set of pca and cat genes that code for complete conversion of catechol to central metabolites. This work contributes in a number of novel and significant ways to our understanding of iac gene biology in bacteria from (non-)plant environments. More specifically, we show that LF7's response to IAA involves derepression of the MarR-type transcriptional regulator IacR, which is quite fast (less than 25 min upon IAA exposure), highly specific (only in response to IAA and chlorinated IAA, and with few genes other than iac, cat, and pca induced), relatively sensitive (low micromolar range), and seemingly tailored to exploit IAA as a source of carbon and energy.

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