scholarly journals Effects of Gibberellic Acid and Gold Light on Germination, Enzyme Activities, and Amino Acid Pool Size in a Dwarf Strain of Watermelon

1978 ◽  
Vol 62 (1) ◽  
pp. 6-9 ◽  
Author(s):  
Kathleen B. Evensen ◽  
J. Brent Loy
Keyword(s):  
Amino Acid ◽  
Gibberellic Acid ◽  
Enzyme Activities ◽  
Pool Size ◽  
Amino Acid Pool ◽  
Acid Pool

scholarly journals The effect of inhibitors in vivo on protein synthesis and the amino acid pool in the sheep blowfly, Lucilia cuprina

1975 ◽  
Vol 150 (2) ◽  
pp. 227-234 ◽  
Author(s):  
A J Campbell ◽  
L M Birt
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Actinomycin D ◽  
Pool Size ◽  
Dynamic State ◽  
Amino Acid Pool ◽  
Pronounced Increase ◽  
Acid Pool ◽  
Quantitative Estimates

1. The rates of detoxification of cycloheximide (33 μg/g fresh wt.), puromycin (167 μg/g fresh wt.) and actinomycin D (1 μg/g fresh wt.) were assessed in vivo on the basis of acid-insoluble [14C]leucine incorporation in the sheep blowfly, Lucilla cuprina; these were compared with quantitative estimates which took account not only of incorporation data but also of leucine pool size and turnover. Quantitatively, cycloheximide and puromycin were still at least 50% effective in inhibiting protein synthesis after 6.5 and 24.5h of exposure respectively, whereas values based only on incorporation data suggested that cycloheximide was 83% effective and puromycin completely ineffective after the respective periods. Quantitative estimates also showed that actinomycin D effectiveness increased with increasing exposure over 24.5h, in contrast with values based only on incorporation data, which suggested that it was completely ineffective after 24h.2. All inhibitors affected the dynamic state of the amino acid pool; there was a marked decrease in the rate of leucine-pool turnover as well as an increase in the half-life of leucine in the pool. 3. Inhibition of protein synthesis resulted in changes in leucine-pool size; the most pronounced increase occurred with cycloheximide and puromycin and the most pronounced decreases with actinomycin D. 4. Evidence is presented which suggests that proteolysis is functionally linked to protein synthesis, which determines its rate indirectly.

Cellular Components Specifically Labeled during Sorbose Stimulation of Sugar Transport in Neurospora

1971 ◽  
Vol 26 (5) ◽  
pp. 409-413 ◽  
Author(s):  
Walter Klingmüller
Keyword(s):  
Amino Acid ◽  
Transport System ◽  
Sugar Transport ◽  
Pool Size ◽  
Amino Acid Pool ◽  
Acid Pool ◽  
Soluble Component ◽  
Cellular Components ◽  
Stimulation Of

A number of independent systems for sugar transport in Neurospora have been postulated. An approach to identification of specific components of the sorbose transport system in ungerminated conidia pretreated with sorbose is described here. The fact that sorbose stimulates sorbose-uptake without causing germination was exploited in differential labeling experiments. Sorbose treated and untreated conidia were incubated with (3H) -leucine and (14C) -leucine respectively, and then mixed extracts fractionated on CM-cellulose at pH 4.75. Changes in the 3H/14C-ratio indicated sorbose-induced or -repressed substances. In selecting labeling conditions, care was taken to avoid errors that might arise from any differences in the internal amino acid pool size.The investigation revealed a buffer-soluble component repressed by sorbose and a Triton-soluble component induced by sorbose. The buffer-soluble, sorbose repressible protein is discussed in connection with the phenomenon of sorbose toxicity in Neurospora. The Triton-soluble, sorbose stimulated component, probably of carotenoid nature, is considered as a possible constituent of the sorbose transport system investigated here.

The occurrence of L-lanthionine in the amino-acid pool of insects

1966 ◽  
Vol 22 (2) ◽  
pp. 163-168 ◽  
Author(s):  
D. Rajagopal Rao ◽  
A.H. Ennor ◽  
B. Thorpe
Keyword(s):  
Amino Acid ◽  
Amino Acid Pool ◽  
Acid Pool

Postnatal changes in free amino acid pool of rabbit brain

1967 ◽  
Vol 3 (4) ◽  
pp. 374-380 ◽  
Author(s):  
Harish C. Agrawal ◽  
Jimmie M. Davis ◽  
Williamina A. Himwich
Keyword(s):  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Amino Acid Pool ◽  
Rabbit Brain ◽  
Free Amino Acid Pool ◽  
Acid Pool
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