scholarly journals Partial Purification of a Growth Factor from Orange Juice Which Affects Citrus Tissue Culture and Its Replacement by Citric Acid

1975 ◽  
Vol 56 (2) ◽  
pp. 279-282 ◽  
Author(s):  
Yair Erner ◽  
Oded Reuveni ◽  
Eliezer E. Goldschmidt
Keyword(s):  
Tissue Culture ◽  
Citric Acid ◽  
Growth Factor ◽  
Orange Juice ◽  
Partial Purification ◽  
Citrus Tissue Culture

An Ultrafilterable Growth Factor for Tissue Culture

Science ◽  
1953 ◽  
Vol 117 (3047) ◽  
pp. 566-566 ◽  
Author(s):  
S. Rosenberg ◽  
P. L. Kirk
Keyword(s):  
Tissue Culture ◽  
Growth Factor

The Effects of Retinoic Acid on the Insulin-like Growth Factor Axis in Primary Tissue Culture from Hyperparathyroidism

2006 ◽  
Vol 30 (5) ◽  
pp. 714-720 ◽  
Author(s):  
Chris K.M. Wong ◽  
Teresa Lai ◽  
Jeffrey M.P. Holly ◽  
Malcolm H. Wheeler ◽  
Claire E.H. Stewart ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Retinoic Acid ◽  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
Primary Tissue Culture ◽  
Primary Tissue

scholarly journals Adsorption of Nerve Growth Factor onto Surfaces Implications for the Assay in Tissue Culture

1973 ◽  
Vol 32 (3) ◽  
pp. 569-575 ◽  
Author(s):  
Frederick L. Pearce ◽  
Derek V. BANTHORPE ◽  
Janet M. Cook ◽  
Charles A. VERNON
Keyword(s):  
Tissue Culture ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Nerve Growth

scholarly journals BIOCHEMICAL STUDY OF CELLULAR ANTIGEN-ANTIBODY REACTION IN TISSUE CULTURE

1960 ◽  
Vol 112 (2) ◽  
pp. 249-255 ◽  
Author(s):  
Akira Tokuda ◽  
Hideo Hayashi ◽  
Kinishiro Matsuba
Keyword(s):  
Tissue Culture ◽  
Protease Activity ◽  
Biochemical Study ◽  
Culture Fluid ◽  
Partial Purification ◽  
Trichloracetic Acid ◽  
Heat Stable ◽  
Antibody Reaction ◽  
Antigen Antibody ◽  
Cellular Antigen

Decrease in the protease activity of the culture fluid observed at later stages of the antigen-antibody reaction is believed to be due to the release of an inhibitor by the cells. The inhibitor was submitted to partial purification: it is heat-stable, non-precipitated by trichloracetic acid and non-dialyzable. It inhibits certain cellular and tissue proteases and papain but is inactive against trypsin. It is suggested that the balance between protease and anti-protease released may determine the intensity, extent, and duration of certain sensitization phenomena.

The effects of fruit juices and fruits on the absorption of iron from a rice meal

1987 ◽  
Vol 57 (3) ◽  
pp. 331-343 ◽  
Author(s):  
D. Ballot ◽  
R. D. Baynes ◽  
T. H. Bothwell ◽  
M. Gillooly ◽  
J. Macfarlane ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Citric Acid ◽  
Malic Acid ◽  
Acid Content ◽  
Orange Juice ◽  
Geometric Mean ◽  
Citrus Fruit ◽  
Fruit Juices ◽  
Persea Americana ◽  
Better Than

1. The effects of the chemical composition of fruit juices and fruit on the absorption of iron from a rice (Oryza sativa) meal were measured in 234 parous Indian women, using the erythrocyte utilization of radioactive Fe method.2. The corrected geometric mean Fe absorptions with different juices varied between 0.040 and 0.129, with the variation correlating closely with the ascorbic acid contents of the juices (rs 0.838, P < 0.01).3. Ascorbic acid was not the only organic acid responsible for the promoting effects of citrus fruit juices on Fe absorption. Fe absorption from laboratory ‘orange juice’ (100 ml water, 33 mg ascorbic acid and 750 mg citric acid) was significantly better than that from 100 ml water and 33 mg ascorbic acid alone (0.097 and 0.059 respectively), while Fe absorption from 100 ml orange juice (28 mg ascorbic acid) was better than that from 100 ml water containing the same amount of ascorbic acid (0.139 and 0.098 respectively). Finally, Fe absorption from laboratory ‘lemon juice’ (100 ml orange juice and 4 g citric acid) was significantly better than that from 100 ml orange juice (0.226 and 0,166 respectively).4. The corrected geometric mean Fe absorption from the rice meal was 0.025. Several fruits had little or no effect on Fe absorption from the meal (0.013–0.024). These included grape (Vitis vinifera), peach (Prunuspersica), apple (Malus sylvestris) and avocado pear (Persea americana). Fruit with a mild to moderate enhancing effect on Fe absorption (0.03 1–0.088) included strawberry (Fragaria sp.) (uncorrected values), plum (Prunus domestica), rhubarb (Rheum rhaponticum), banana (Musa cavendishii), mango (Mangifera indica), pear (Pyrus cornmunis), cantaloup (Cucumis melo) and pineapple (Ananas comosus) (uncorrected values). Guava (Psidium guajava) and pawpaw (Carica papaya) markedly increased Fe absorption (0.126–0.293).5. There was a close correlation between Fe absorption and the ascorbic acid content of the fruits tested (rs 0.738, P < 0.0001). There was also a weaker but significant correlation with the citric acid content (rs 0.55, P < 0.03). Although this may have reflected a direct effect of citric acid on Fe absorption, it should be noted that fruits containing citric acid also contained ascorbic acid (rs 0.70, P < 0.002). Similarly, the negative correlation (rs –0.62, P < 0,008) between Fe absorption and the malic acid content of fruits may have been due to the fact that fruits with a high malic acid content tended to have low levels of ascorbic acid (rs–0.45, P < 0.06).6. These various results suggested that most fruits have only a limited effect on overall Fe nutrition. However, the presence of citrus fruit, guava or pawpaw would be expected to increase Fe absorption markedly from diets of low Fe availability.

scholarly journals A novel human cell culture model to study visceral smooth muscle phenotypic modulation in health and disease

2018 ◽  
Vol 315 (4) ◽  
pp. C598-C607 ◽  
Author(s):  
Rianne D. W. Vaes ◽  
Linda van den Berk ◽  
Bas Boonen ◽  
David P. J. van Dijk ◽  
Steven W. M. Olde Damink ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Smooth Muscle ◽  
Growth Factor ◽  
Muscle Protein ◽  
Smooth Muscle Actin ◽  
Muscle Actin ◽  
Phenotypic Modulation ◽  
Culture Model ◽  
Health And Disease ◽  
Visceral Smooth Muscle

Adaptation of the smooth muscle cell (SMC) phenotype is essential for homeostasis and is often involved in pathologies of visceral organs (e.g., uterus, bladder, gastrointestinal tract). In vitro studies of the behavior of visceral SMCs under (patho)-physiological conditions are hampered by a spontaneous, uncontrolled phenotypic modulation of visceral SMCs under regular tissue culture conditions. We aimed to develop a new visceral SMC culture model that allows controlled phenotypic modulation. Human uterine SMCs [ULTR and telomerase-immortalized human myometrial cells (hTERT-HM)] were grown to confluency and kept for up to 6 days on regular tissue culture surfaces or basement membrane (BM) matrix-coated surfaces in the presence of 0–10% serum. mRNA and protein expression and localization of SMC-specific phenotype markers and their transcriptional regulators were investigated by quantitative PCR, Western blotting, and immunofluorescence. Maintaining visceral SMCs confluent for 6 days increased α-smooth muscle actin (1.9-fold) and smooth muscle protein 22-α (3.1-fold), whereas smooth muscle myosin heavy chain was only slightly upregulated (1.3-fold). Culturing on a BM matrix-coated surface further increased these proteins and also markedly promoted mRNA expression of γ-smooth muscle actin (15.0-fold), smoothelin (3.5-fold), h-caldesmon (5.2-fold), serum response factor (7.6-fold), and myocardin (8.1-fold). Whereas additional serum deprivation only minimally affected contractile markers, platelet-derived growth factor-BB and transforming growth factor β1 consistently reduced versus increased their expression. In conclusion, we present a simple and reproducible visceral SMC culture system that allows controlled phenotypic modulation toward both the synthetic and the contractile phenotype. This may greatly facilitate the identification of factors that drive visceral SMC phenotypic changes in health and disease.

Sign in / Sign up

Export Citation Format

Share Document