scholarly journals Photosynthetic Activities of Spinach Leaf Protoplasts

1975 ◽  
Vol 55 (4) ◽  
pp. 712-716 ◽  
Author(s):  
Mikio Nishimura ◽  
Takashi Akazawa
Keyword(s):  
Spinach Leaf ◽  
Photosynthetic Activities ◽  
Leaf Protoplasts

scholarly journals Translocation of Photosynthates into Vacuoles in Spinach Leaf Protoplasts

1985 ◽  
Vol 77 (4) ◽  
pp. 963-968 ◽  
Author(s):  
Sumio Asami ◽  
Ikuko Hara-Nishimura ◽  
Mikio Nishimura ◽  
Takashi Akazawa
Keyword(s):  
Spinach Leaf ◽  
Leaf Protoplasts

Endogenous biosynthetic precursors of (+)-abscisic acid. VI. Carotenoids and ABA are formed by the ‘non-mevalonate’ triose-pyruvate pathway in chloroplasts

1998 ◽  
Vol 25 (5) ◽  
pp. 507 ◽  
Author(s):  
B.V. Milborrow ◽  
H.-S. Lee
Keyword(s):  
Abscisic Acid ◽  
Leaf Tissue ◽  
Isopentenyl Diphosphate ◽  
Free System ◽  
Cell Free System ◽  
Avocado Fruit ◽  
Intact Chloroplasts ◽  
A Cell ◽  
Spinach Leaf ◽  
Leaf Protoplasts

A cell-free system from avocado fruit which routinely incorporated [14C]mevalonate into ABA (1000 dpm per 5 mL of preparation), and into carotenoids, has now been shown to incorporate [14C]pyruvate even more successfully (1620 dpm). Intact chloroplasts from spinach leaf protoplasts incorporated 2990 dpm of [14C]pyruvate (from 2 x 106 dpm) into ABA compared with 990 dpm from [3-R-5-14C]mevalonate (also from 2 x 106 dpm). The intact chloroplasts also produced [14C]ABA (1575 dpm) when supplied with [14C]isopentenyl diphosphate. This result establishes that the whole pathway of biosynthesis of ABA can occur within chloroplasts. Little [14C]acetate or [14C]alanine was incorporated into ABA by avocado fruit mesocarp. Most of the ABA in leaf tissue now appears to be formed by the triose-pyruvate pathway in chloroplasts and incorporation of [14C]mevalonate occurs after activation in the cytoplasm and importation of a later intermediate into the plastids.

scholarly journals Effect of Oxygen on Photosynthesis by Spinach Leaf Protoplasts

1975 ◽  
Vol 56 (5) ◽  
pp. 718-722 ◽  
Author(s):  
Mikio Nishimura ◽  
Douglas Graham ◽  
Takashi Akazawa
Keyword(s):  
Spinach Leaf ◽  
Effect Of Oxygen ◽  
Leaf Protoplasts

Measurement of the ascorbate content of spinach leaf protoplasts and chloroplasts during illumination

Planta ◽  
1983 ◽  
Vol 157 (3) ◽  
pp. 239-244 ◽  
Author(s):  
Christine Foyer ◽  
Jackie Rowell ◽  
David Walker
Keyword(s):  
Spinach Leaf ◽  
Leaf Protoplasts

scholarly journals A Simple Method for Estimating Intactness of Spinach Leaf Protoplasts by Glycolate Oxidase Assay

1985 ◽  
Vol 78 (2) ◽  
pp. 343-346 ◽  
Author(s):  
Mikio Nishimura ◽  
Roland Douce ◽  
Takashi Akazawa
Keyword(s):  
Glycolate Oxidase ◽  
Simple Method ◽  
Spinach Leaf ◽  
Leaf Protoplasts

scholarly journals Biosynthesis of Ribulose-1,5-Bisphosphate Carboxylase in Spinach Leaf Protoplasts

1978 ◽  
Vol 62 (1) ◽  
pp. 97-100 ◽  
Author(s):  
Mikio Nishimura ◽  
Takashi Akazawa
Keyword(s):  
Bisphosphate Carboxylase ◽  
Spinach Leaf ◽  
Leaf Protoplasts

scholarly journals Isolation and Characterization of Metabolically Competent Mitochondria from Spinach Leaf Protoplasts

1982 ◽  
Vol 69 (4) ◽  
pp. 916-920 ◽  
Author(s):  
Mikio Nishimura ◽  
Roland Douce ◽  
Takashi Akazawa
Keyword(s):  
Isolation And Characterization ◽  
Spinach Leaf ◽  
Leaf Protoplasts

Variation in Chloroplast Lipid Content and its Correlation to Photosynthetic Activities

1978 ◽  
Vol 33 (9-10) ◽  
pp. 685-687 ◽  
Author(s):  
K.-P. Heise
Keyword(s):  
Membrane Lipids ◽  
Neutral Lipids ◽  
Lipid Fraction ◽  
Lipid Synthesis ◽  
Neutral Lipid Fraction ◽  
Intact Chloroplasts ◽  
Spinach Leaf ◽  
Fixation Capacity ◽  
Photosynthetic Activities ◽  
Proportional Decrease

Abstract During illumination infiltrated spinach leaf sections show after a short log a linearly increasing 14C-incorporation into membrane lipids from H14CO3-.Isolated intact chloroplasts show this increase only for the neutral lipid fraction (NL). Glyco-and phospholipid accumulation declines in the light.In chloroplast suspensions the increase in content and also in its 14C-incorporation rate of the NL fraction is accompanied1. by a proportional decrease of CO2-fixation capacity,2. by a decrease of the P/2 e-quotient.From 14C-incorporation kinetics, this increase of neutral lipids in plastid suspensions even after short illumination periods appears to be a consequence of an inhibited glycolipid - and phospho­ lipid synthesis from diglycerides.

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