scholarly journals Higher Plant Cell Membrane Resistance by a Single Intracellular Electrode Method

1974 ◽  
Vol 53 (1) ◽  
pp. 122-124 ◽  
Author(s):  
W. P. Anderson ◽  
D. L. Hendrix ◽  
N. Higinbotham
1971 ◽  
Vol 49 (5) ◽  
pp. 436-447 ◽  
Author(s):  
D. R. Firth ◽  
L. J. DeFelice

The electrical resistance and the flow of solution in glass microelectrodes has been studied as function of the concentration of internal and external solution (KCl, 3–10−5 M), and as a function of pressure between the inside and outside solutions. The study demonstrates the usefulness of using microelectrodes for measuring cell membrane resistance and also the dangers in using them as microejectors because of anomalous flow effects. Electrokinetic effects and tip potentials are briefly described; they offer a means of investigating the surface condition of the glass wall inside the tip of the electrode and its variation with the outside solution.


Zygote ◽  
2019 ◽  
Vol 27 (4) ◽  
pp. 250-254
Author(s):  
Mei Goto ◽  
Hirokatsu Saito ◽  
Yuki Hiradate ◽  
Kenshiro Hara ◽  
Kentaro Tanemura

SummaryOocytes of B6D2F1 (BDF1) mice are often used as recipients for intracytoplasmic sperm injection because of their cell membrane resistance against capillary penetration. It is assumed that oocytes of BDF1 mice have superior traits because of their hybrid vigour. However, the mechanisms of hybrid vigour are unclear. In this study, we focused on the membrane resistance of MII stage oocytes against changes in extracellular osmotic pressure. As a result, MII stage oocytes of inbred C57BL/6 and DBA/2 mice showed high tolerance in either a hypertonic or a hypotonic environment. Conversely, MII stage oocytes of hybrid BDF1 and D2B6F1 mice showed high tolerance in both hypertonic and hypotonic environments. Therefore, it is considered that MII stage oocytes of hybrid mice have superior traits than those of inbred mice. Our findings demonstrated that the hybrid vigour exists in the form of resistance to extracellular osmotic environment in hybrid MII stage oocytes.


2014 ◽  
Vol 171 (9) ◽  
pp. 748-769 ◽  
Author(s):  
Anne-Aliénor Véry ◽  
Manuel Nieves-Cordones ◽  
Meriem Daly ◽  
Imran Khan ◽  
Cécile Fizames ◽  
...  

1979 ◽  
pp. 45-76 ◽  
Author(s):  
C. M. Rivera ◽  
Donald Penner

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