scholarly journals The Osmotic Potential of Polyethylene Glycol 6000

1973 ◽  
Vol 51 (5) ◽  
pp. 914-916 ◽  
Author(s):  
Burlyn E. Michel ◽  
Merrill R. Kaufmann
Keyword(s):  
Polyethylene Glycol ◽  
Osmotic Potential ◽  
Polyethylene Glycol 6000

scholarly journals Effects of Osmopriming on Germination and Vigour Traits of Bersim Clover (Trifolium alexandrinum L.)

2010 ◽  
Vol 2 (4) ◽  
pp. 59-63 ◽  
Author(s):  
Hossein Reza ROUHI ◽  
Reza Tavakkol AFSHARI ◽  
Seyed Amir MOOSAVI ◽  
Mohammad Hossain GHARINEH
Keyword(s):  
Polyethylene Glycol ◽  
Osmotic Potential ◽  
Seed Priming ◽  
Control Group ◽  
Trifolium Alexandrinum ◽  
Distilled Water ◽  
Peg 6000 ◽  
Crop Establishment ◽  
Germination Characteristics ◽  
Polyethylene Glycol 6000

Previous studies suggested that fast and uniform germination is important for good crop establishment. The present study was conducted to investigate the possibility of increasing germination characteristics by seed priming techniques. An experiment was conducted with three replicates and two treatments including: 2 different priming duration (8 and 12 hours) and 6 osmotic potential of PEG 6000 solutions (-0.8, -1,-1.2,-1.4,-1.6 Mpa) and distilled water as a control group). The priming solutions were prepared using polyethylene glycol 6000 (PEG). Our results showed that the most effective osmotic potential in improving germination characteristics of Trifolium alexandrium is -0.8 MPa for 16 hours.

Effect of different priming treatments and priming durations on melon germination behavior under suboptimal conditions

2018 ◽  
Vol 3 (1) ◽  
pp. 386-392 ◽  
Author(s):  
J. L. Castañares ◽  
C. A. Bouzo
Keyword(s):  
Polyethylene Glycol ◽  
Low Temperature ◽  
Temperature Stress ◽  
Osmotic Potential ◽  
Germination Percentage ◽  
Control Treatment ◽  
Saline Stress ◽  
Mean Germination Time ◽  
Osmotic Agents ◽  
Polyethylene Glycol 6000

AbstractThe objective of this work was to compare the effect of different priming osmotic agents and durations on melon germination in: 1) low temperature stress; 2) saline stress; and 3) low temperature and saline stress. The osmotic agents were polyethylene glycol 6000, KNO3+K3PO4, CaCl2and NaCl, with -1.5 MPa osmotic potential. Priming durations were 3 and 6 days (d). Germination percentage (GP) and mean germination time (MGT) were measured. At 12°C the best GP was 14% with CaCl2/3-d, without germination in control. At 25°C the best GP was 100% with CaCl2/3-d. MGT was reduced one day. At -0.7 MPa GP was 100% with CaCl2/3-d and NaCl 3d. At -1.0 MPa the best GP were 46 and 50% for 3d with NaCl and CaCl2respectively without germination in control treatment. At 12°C and -1.0 MPa the best GP were CaCl2/3-d and CaCl2/3-d (14 and 10% respectively). It is concluded that at 12°C, the increase in GP would not justify the use of priming. At 25°C priming increased GP and reduced MGT. At -0.7 MPa priming increased germination, while at -1.0 MPa the increase is not agronomical considerable. At 12°C and -1.0 MPa the increase of germination is not agronomical important.

Hepatitis a Virus Concentration from Experimentally Contaminated Distilled, Tap, Waste and Seawater

1989 ◽  
Vol 21 (3) ◽  
pp. 255-258 ◽  
Author(s):  
Evangélos Biziagos ◽  
Jacques Passagot ◽  
Jean-Marc Crance ◽  
Robert Deloince
Keyword(s):  
Cell Culture ◽  
Polyethylene Glycol ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Tap Water ◽  
Virus Concentration ◽  
Beef Extract ◽  
Polyethylene Glycol 6000

The concentration of cell-culture-adapted hepatitis A virus (HAV) from experimentally contaminated distilled, drinking, waste and seawater was performed by using a filter adsorption-elu-tion method in the following conditions: HAV seeded in water was adsorbed at pH 4.0 to two nitrocellulose membranes (1.2 and 0.45 µm porosity for distilled and tap water or 8.0 and 3.0 µm porosity for waste and seawater), then eluted by 3% beef-extract at pH 8.5 and further concentrated by polyethylene glycol 6000 precipitation. Thus, HAV in 5 to 50 liters of seeded waters was concentrated approximately 1,700 to 17,000 fold with greater than 70% recovery of the initial virus added to the samples.

Effect of Alachlor on PEG6000Uptake, Root Osmotic Potential, and Root Leakage

Weed Science ◽  
1983 ◽  
Vol 31 (5) ◽  
pp. 600-603 ◽  
Author(s):  
Charles S. Vavrina ◽  
Richard A. Ashley
Keyword(s):  
Zea Mays ◽  
Glycine Max ◽  
Polyethylene Glycol ◽  
Osmotic Potential ◽  
Electrolyte Leakage ◽  
Sweet Corn ◽  
Root Electrolyte Leakage ◽  
Soybean Roots ◽  
Nutrient Solutions

Sweet corn (Zea maysL.rugosa'Seneca Scout’) and soybeans (Glycine maxL. Merr. ‘Kanrich’) were grown in nutrient solutions containing alachlor [2-chloro-2′,6-diethyl-N-(methoxymethyl)acetanilide] at 2.36 × 10-5M, polyethylene glycol MW 6000 (PEG6000) at −2.5 bars osmotic potential, alachlor at 2.36 × 10-5M plus PEG6000at −2.5 bars osmotic potential or with no additions. Sweet corn and soybean roots exposed to alachlor plus PEG6000accumulated significantly more PEG6000than roots exposed to PEG6000alone; increased shoot uptake occurred only in soybean. Alachlor was also shown to significantly increase root osmotic potential and decrease root electrolyte leakage.

A new automated turbidimetric immunoassay for quantifying alpha 1-antitrypsin in serum.

1986 ◽  
Vol 32 (6) ◽  
pp. 1020-1022 ◽  
Author(s):  
J A Viedma ◽  
A de la Iglesia ◽  
M Parera ◽  
M T López
Keyword(s):  
Polyethylene Glycol ◽  
Parametric Estimation ◽  
Tween 20 ◽  
Upper Limit ◽  
Analytical Recovery ◽  
The Mean ◽  
Alpha 1 Antitrypsin ◽  
Polyethylene Glycol 6000 ◽  
Immunoprecipitation Reaction ◽  
Turbidimetric Immunoassay

Abstract This rapid, sensitive equilibrium turbidimetric immunoassay for quantification of alpha 1-antitrypsin involves a monospecific antibody, polyethylene glycol 6000 to accelerate and enhance the immunoprecipitation reaction, and Tween 20 surfactant to decrease and stabilize the sample-blank values. Turbidity at 334 nm is measured by an automated discrete analyzer. Grossly lipemic, icteric, or hemolyzed samples can be assayed. Correlation with results by radial immunodiffusion (RID) was excellent (r = 0.97, n = 84). Analytical recovery averaged 97.7 (SD 2.9)%. Within-run CVs ranged from 1.6 to 1.9%, between-day CVs from 2.0 to 3.5%. Reference values for healthy adults (n = 147) were determined by parametric estimation (for an assumed normal distribution of untransformed data). The lower limit (g/L) with its 0.90 confidence interval is 1.23 (range 1.18-1.28), the upper limit is 2.15 (2.10-2.20), and the mean is 1.69 g/L.

N-Ethylmaleimide-modified actin filaments do not bundle in the presence of α-actinin

1995 ◽  
Vol 73 (1-2) ◽  
pp. 116-122 ◽  
Author(s):  
Aldo Milzani ◽  
Isabella DalleDonne ◽  
Roberto Colombo
Keyword(s):  
Polyethylene Glycol ◽  
Chemical Modification ◽  
Molecular Interactions ◽  
Binding Sites ◽  
Actin Filaments ◽  
Actin Assembly ◽  
Chemically Modified ◽  
C Terminus ◽  
Spatial Reorganization ◽  
Polyethylene Glycol 6000

We show that the modification of actin subdomain 1 by N-ethylmaleimide (NEM), which binds Cys-374 close to the C-terminus of the molecule, inhibits the α-actinin-induced bundling of actin filaments. This effect is not merely related to the block of Cys-374, since N-(1-pyrenyl)iodoacetamide (pyrene-IA) is unable to prevent bundling. Considering that NEM (but not pyrene-IA) influences actin assembly, we suggest that the inhibition of the actin – α-actinin interaction is due to the chemical modification of actin Cys-374 which, by inducing a marked spatial reorganization of actin monomers, is able to modify both the intra- and inter-molecular interactions of this protein. Finally, NEM-modified actin filaments form bundles in the presence of polyethylene glycol 6000 since, in this case, the side by side association of actin filaments does not depend on the accessibility of binding sites nor on the formation of chemical bonds.Key words: chemically modified actin, N-ethylmaleimide, pyrene-IA, Cys-374, actin bundles, α-actinin.

scholarly journals Rapid Assay of Trace Immunoglobulin M by a New Immunonanogold Resonance Scattering Spectral Probe

2008 ◽  
Vol 13 (4) ◽  
pp. 302-308 ◽  
Author(s):  
Zhiliang Jiang ◽  
Lili Wei ◽  
Mingjing Zou ◽  
Aihui Liang ◽  
Mianwu Meng
Keyword(s):  
Polyethylene Glycol ◽  
Detection Limit ◽  
Resonance Scattering ◽  
Immunoglobulin M ◽  
Serum Samples ◽  
Rapid Assay ◽  
Lower Detection Limit ◽  
Lower Detection ◽  
Polyethylene Glycol 6000

Nanogold, 8 nm in size, was used to label goat antihuman immunoglobulin M (GIgM) to obtain a new immunonanogold resonance scattering (RS) probe (Au-GIgG) for quantitation of trace immunoglobulin M (IgM). The Au-GIgG combined with IgM to form nanogold-labeled immunocomplex causes the RS intensity at 580 nm to be enhanced, in pH 4.49 KH2PO 4-Na2HPO4 buffer and in the presence of polyethylene glycol 6000. The enhanced RS intensity at 580 nm (ΔI580 nm) is proportional to the IgM concentration in the range of 1.5 to 2000 ng/mL, with a lower detection limit of 0.98 ng/mL. The immunonanogold RS assay was used to assay IgM in serum samples, with sensitivity, selectivity, and simplicity. ( Journal of Biomolecular Screening 2008:302-308)

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