scholarly journals In Vivo Assay of Nitrate Reductase in Cotton Leaf Discs

1973 ◽  
Vol 51 (2) ◽  
pp. 332-336 ◽  
Author(s):  
J. W. Radin
Keyword(s):  
Nitrate Reductase ◽  
Cotton Leaf ◽  
Leaf Discs ◽  
In Vivo Assay

In vivo assay of nitrate reductase in tea (Camellia sinensis L.) leaf and root

1987 ◽  
Vol 41 (2) ◽  
pp. 107-113 ◽  
Author(s):  
Tarun Ch. Sarmah ◽  
Mahinath Dev Choudhury ◽  
Monoranjan Goswami
Keyword(s):  
Nitrate Reductase ◽  
Camellia Sinensis ◽  
In Vivo Assay

In vivo Nitrate Reductase Activity in Leaves of Pearl Millet, Pennisetum americanum (L.) Leeke

1987 ◽  
Vol 14 (2) ◽  
pp. 125 ◽  
Author(s):  
SV Chanda ◽  
AK Joshi ◽  
PN Krishnan ◽  
YD Singh
Keyword(s):  
Nitrate Reductase ◽  
Reductase Activity ◽  
Potassium Phosphate ◽  
Limiting Factor ◽  
Growth Stages ◽  
In Vivo Assay ◽  
Rate Limiting ◽  
Nr Activity

In the in vivo assay of nitrate reductase (NR) in P. americanum leaves, addition of 1% (v/v) Triton X-100, potassium phosphate buffer (80 mM, pH 7.4) and 1.13 mM NADH to the assay medium resulted in maximum activity. With increasing concentration of NADH, saturation-type kinetics were observed. Based on this data metabolic pool concentration for NADH and apparent Km for nitrate reductase were determined. In field studies with cultivars BJ-104, J-104 and 5141-A of P. americanum, the relative limitation of NO3-, NADH and nitrate reductase in NO3- assimilation was determined. NR activity was measured by four modifications of the in vivo assay technique (with NO3-, with NADH, without NO3- and NADH and with both NO3- and NADH additions to the reaction mixture) and with one in vitro technique. For all the cultivars, NADH was the major rate-limiting factor for in vivo assay during early growth stages, while at later stages, NO3- was limiting. At no stage was NR rate-limiting. It is concluded that NR activity alone may not serve as biochemical marker for improved efficiency of utilisation of nitrogen in P. americanum.

scholarly journals Some New Aspects of the in Vivo Assay for Nitrate Reductase in Wheat (Triticum aestivum L.) Leaves

1980 ◽  
Vol 65 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Richard H. Hageman ◽  
Andrew J. Reed ◽  
Rise A. Femmer ◽  
Joseph H. Sherrard ◽  
Michael J. Dalling
Keyword(s):  
Triticum Aestivum ◽  
Nitrate Reductase ◽  
Triticum Aestivum L ◽  
In Vivo Assay
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